Construct: ORF TRCN0000487828
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020461.1_s317c1
- DNA Barcode:
- GCGGTGCAAGTAACACGGGGCACT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K20 (51776)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487828
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51776 | MAP3K20 | mitogen-activated protein k... | NM_133646.3 | 99.9% | 100% | 439T>C |
| 2 | human | 51776 | MAP3K20 | mitogen-activated protein k... | XM_017004323.1 | 99.9% | 100% | 439T>C |
| 3 | human | 51776 | MAP3K20 | mitogen-activated protein k... | XM_017004324.1 | 99.9% | 100% | 439T>C |
| 4 | human | 51776 | MAP3K20 | mitogen-activated protein k... | NM_016653.3 | 49.7% | 44.6% | (many diffs) |
| 5 | human | 51776 | MAP3K20 | mitogen-activated protein k... | XM_005246640.2 | 49.7% | 44.6% | (many diffs) |
| 6 | mouse | 65964 | Map3k20 | mitogen-activated protein k... | NM_001164791.1 | 93.1% | 96.4% | (many diffs) |
| 7 | mouse | 65964 | Map3k20 | mitogen-activated protein k... | NM_178084.4 | 59.4% | 61.9% | (many diffs) |
| 8 | mouse | 65964 | Map3k20 | mitogen-activated protein k... | NM_023057.5 | 47.5% | 43.6% | (many diffs) |
| 9 | mouse | 65964 | Map3k20 | mitogen-activated protein k... | XM_006499997.2 | 47.5% | 43.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1437
- ORF length:
- 1365
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcgtct ctcggtgcct cctttgtgca aattaaattt gatgacttgc 121 agttttttga aaactgcggt ggaggaagtt ttgggagtgt ttatcgagcc aaatggatat 181 cacaggacaa ggaggtggct gtaaagaagc tcctcaaaat agagaaagag gcagaaatac 241 tcagtgtcct cagtcacaga aacatcatcc agttttatgg agtaattctt gaacctccca 301 actatggcat tgtcacagaa tatgcttctc tgggatcact ctatgattac attaacagta 361 acagaagtga ggagatggat atggatcaca ttatgacctg ggccactgat gtagccaaag 421 gaatgcatta tttacatatg gaggctcctg tcaaggtgat tcacagagac ctcaagtcaa 481 gaaacgttgt tatagctgct gatggagtac tgaagatctg tgactttggt gcctctcggt 541 tccataacca tacaacacac atgtccttgg ttggaacttt cccatggatg gctccagaag 601 ttatccagag tctccctgtg tcagaaactt gtgacacata ttcctatggt gtggttctct 661 gggagatgct aacaagggag gtccccttta aaggtttgga aggattacaa gtagcttggc 721 ttgtagtgga aaaaaacgag agattaacca ttccaagcag ttgccccaga agttttgctg 781 aactgttaca tcagtgttgg gaagctgatg ccaagaaacg gccatcattc aagcaaatca 841 tttcaatcct ggagtccatg tcaaatgaca cgagccttcc tgacaagtgt aactcattcc 901 tacacaacaa ggcggagtgg aggtgcgaaa ttgaggcaac tcttgagagg ctaaagaaac 961 tagagcgtga tctcagcttt aaggagcagg agcttaaaga acgagaaaga cgtttaaaga 1021 tgtgggagca aaagctgaca gagcagtcca acaccccgct tctcttgcct cttgctgcaa 1081 gaatgtctga ggagtcttac tttgaatcta aaacagagga gtcaaacagt gcagagatgt 1141 catgtcagat cacagcaaca agtaacggGG AGGGCCATGG CATGAACCCA AGTCTGCAGG 1201 CCATGATGCT GATGGGCTTT GGGGATATCT TCTCAATGAA CAAAGCAGGA GCTGTGATGC 1261 ATTCTGGGAT GCAGATAAAC ATGCAAGCCA AGCAGAATTC TTCCAAAACC ACATCTAAGA 1321 GAAGGGGGAA GAAAGTCAAC ATGGCTCTGG GGTTCAGTGA TTTTGACTTG TCAGAAGGTG 1381 ACGATGATGA TGATGATGAC GGTGAGGAGG AGGATAATGA CATGGATAAT AGTGAATAGG 1441 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1501 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1561 TTTATATATC TTGTGGAAAG GACGAGCGGT GCAAGTAACA CGGGGCACTA CGCGTTAAGT 1621 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt