Construct: ORF TRCN0000487828

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020461.1_s317c1
DNA Barcode:
GCGGTGCAAGTAACACGGGGCACT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
MAP3K20 (51776)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487828
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51776 MAP3K20 mitogen-activated protein k... NM_133646.3 99.9% 100% 439T>C
2 human 51776 MAP3K20 mitogen-activated protein k... XM_017004323.1 99.9% 100% 439T>C
3 human 51776 MAP3K20 mitogen-activated protein k... XM_017004324.1 99.9% 100% 439T>C
4 human 51776 MAP3K20 mitogen-activated protein k... NM_016653.3 49.7% 44.6% (many diffs)
5 human 51776 MAP3K20 mitogen-activated protein k... XM_005246640.2 49.7% 44.6% (many diffs)
6 mouse 65964 Map3k20 mitogen-activated protein k... NM_001164791.1 93.1% 96.4% (many diffs)
7 mouse 65964 Map3k20 mitogen-activated protein k... NM_178084.4 59.4% 61.9% (many diffs)
8 mouse 65964 Map3k20 mitogen-activated protein k... NM_023057.5 47.5% 43.6% (many diffs)
9 mouse 65964 Map3k20 mitogen-activated protein k... XM_006499997.2 47.5% 43.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1437
ORF length:
1365
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgtcgtct ctcggtgcct cctttgtgca aattaaattt gatgacttgc
121agttttttga aaactgcggt ggaggaagtt ttgggagtgt ttatcgagcc aaatggatat
181cacaggacaa ggaggtggct gtaaagaagc tcctcaaaat agagaaagag gcagaaatac
241tcagtgtcct cagtcacaga aacatcatcc agttttatgg agtaattctt gaacctccca
301actatggcat tgtcacagaa tatgcttctc tgggatcact ctatgattac attaacagta
361acagaagtga ggagatggat atggatcaca ttatgacctg ggccactgat gtagccaaag
421gaatgcatta tttacatatg gaggctcctg tcaaggtgat tcacagagac ctcaagtcaa
481gaaacgttgt tatagctgct gatggagtac tgaagatctg tgactttggt gcctctcggt
541tccataacca tacaacacac atgtccttgg ttggaacttt cccatggatg gctccagaag
601ttatccagag tctccctgtg tcagaaactt gtgacacata ttcctatggt gtggttctct
661gggagatgct aacaagggag gtccccttta aaggtttgga aggattacaa gtagcttggc
721ttgtagtgga aaaaaacgag agattaacca ttccaagcag ttgccccaga agttttgctg
781aactgttaca tcagtgttgg gaagctgatg ccaagaaacg gccatcattc aagcaaatca
841tttcaatcct ggagtccatg tcaaatgaca cgagccttcc tgacaagtgt aactcattcc
901tacacaacaa ggcggagtgg aggtgcgaaa ttgaggcaac tcttgagagg ctaaagaaac
961tagagcgtga tctcagcttt aaggagcagg agcttaaaga acgagaaaga cgtttaaaga
1021tgtgggagca aaagctgaca gagcagtcca acaccccgct tctcttgcct cttgctgcaa
1081gaatgtctga ggagtcttac tttgaatcta aaacagagga gtcaaacagt gcagagatgt
1141catgtcagat cacagcaaca agtaacggGG AGGGCCATGG CATGAACCCA AGTCTGCAGG
1201CCATGATGCT GATGGGCTTT GGGGATATCT TCTCAATGAA CAAAGCAGGA GCTGTGATGC
1261ATTCTGGGAT GCAGATAAAC ATGCAAGCCA AGCAGAATTC TTCCAAAACC ACATCTAAGA
1321GAAGGGGGAA GAAAGTCAAC ATGGCTCTGG GGTTCAGTGA TTTTGACTTG TCAGAAGGTG
1381ACGATGATGA TGATGATGAC GGTGAGGAGG AGGATAATGA CATGGATAAT AGTGAATAGG
1441ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1501GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1561TTTATATATC TTGTGGAAAG GACGAGCGGT GCAAGTAACA CGGGGCACTA CGCGTTAAGT
1621Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)