Construct: ORF TRCN0000487834
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021642.1_s317c1
- DNA Barcode:
- CGTCGGGTCTGGAAGATCCCGAAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- KLF2 (10365)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487834
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10365 | KLF2 | Kruppel like factor 2 | NM_016270.4 | 99.9% | 99.7% | 311T>C |
| 2 | mouse | 16598 | Klf2 | Kruppel-like factor 2 (lung) | NM_008452.2 | 82.2% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1134
- ORF length:
- 1065
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcgctgagt gaacccatcc tgccgtcctt ctccactttc gccagcccgt 121 gccgcgagcg cggcctgcag gagcgctggc cgcgcgccga acccgagtcc ggcggcaccg 181 acgacgacct caacagcgtg ctggacttca tcctgtccat ggggctggat ggcctgggcg 241 ccgaggccgc cccggagccg ccgccgccgc ccccgccgcc tgcgttctat taccccgaac 301 ccggcgcgcc cccgccctac agcgcccccg cgggtggcct ggtgtctgag ctgctgcgac 361 ccgagctgga tgcgccgccg gggcccgcac tgcacggccg ctttctgctg gcgccgcccg 421 gccgcctggt caaggccgag ccccctgaag cggacggcgg cggcggctac ggctgcgccc 481 ccgggctgac ccgtggaccg cgcggcctca agcgcgaggg cgccccgggc ccggcggctt 541 cgtgcatgcg aggtcccggg ggccgccccc cgccgccgcc cgacacaccg ccgctcagcc 601 ccgacggccc cgcgcgcctg cccgcgcccg gtccgcgcgc ctccttcccg ccgcctttcg 661 gtggccctgg tttcggcgcg cccgggcccg gcctgcatta cgcgccgcct gcgcccccag 721 ccttcggtct cttcgacgac gcggccgccg ccgcggcagc cctgggcctg gcgccccccg 781 ccgcccgcgg tctcctcacg ccgcctgcgt ccccgctgga gctgctggag gccaagccaa 841 agcgcggccg ccgctcttgg ccccgcaaac gcaccgccac tcacacctgc agctacgcgg 901 gcTGCGGCAA GACCTACACC AAGAGTTCGC ATCTGAAGGC GCATCTGCGC ACGCACACAG 961 GTGAGAAGCC CTACCACTGC AACTGGGACG GCTGCGGCTG GAAGTTTGCG CGCTCAGACG 1021 AGCTCACGCG CCACTACCGA AAGCACACGG GCCACCGGCC ATTCCAGTGC CATCTGTGCG 1081 ATCGTGCCTT CTCGCGCTCC GATCACCTGG CGCTGCACAT GAAACGGCAC ATGTAGGACC 1141 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GACGTCGGGT CTGGAAGATC CCGAAAACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt