Construct: ORF TRCN0000487842
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019541.1_s317c1
- DNA Barcode:
- AAAATATTATGTTCCTGCAGCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- F2R (2149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487842
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2149 | F2R | coagulation factor II throm... | NM_001992.5 | 99.9% | 99.7% | 1275_1276insG |
| 2 | human | 2149 | F2R | coagulation factor II throm... | NM_001311313.2 | 71.4% | 71.3% | 0_1ins363;912_913insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1353
- ORF length:
- 1278
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgggg ccgcggcggc tgctgctggt ggccgcctgc ttcagtctgt 121 gcggcccgct gttgtctgcc cgcacccggg cccgcaggcc agaatcaaaa gcaacaaatg 181 ccaccttaga tccccggtca tttcttctca ggaaccccaa tgataaatat gaaccatttt 241 gggaggatga ggagaaaaat gaaagtgggt taactgaata cagattagtc tccatcaata 301 aaagcagtcc tcttcaaaaa caacttcctg cattcatctc agaagatgcc tccggatatt 361 tgaccagctc ctggctgaca ctctttgtcc catctgtgta caccggagtg tttgtagtca 421 gcctcccact aaacatcatg gccatcgttg tgttcatcct gaaaatgaag gtcaagaagc 481 cggcggtggt gtacatgctg cacctggcca cggcagatgt gctgtttgtg tctgtgctcc 541 cctttaagat cagctattac ttttccggca gtgattggca gtttgggtct gaattgtgtc 601 gcttcgtcac tgcagcattt tactgtaaca tgtacgcctc tatcttgctc atgacagtca 661 taagcattga ccggtttctg gctgtggtgt atcccatgca gtccctctcc tggcgtactc 721 tgggaagggc ttccttcact tgtctggcca tctgggcttt ggccatcgca ggggtagtgc 781 ctctgctcct caaggagcaa accatccagg tgcccgggct caacatcact acctgtcatg 841 atgtgctcaa tgaaaccctg ctcgaaggct actatgccta ctacttctca gccttctctg 901 ctgtcttctt ttttgtgccg ctgatcattt ccacggtctg ttatgtgtct atcattcgat 961 gtcttagctc ttccgcagtt gccaaccgca gcaagaagtc ccgggctttg ttcctgtcag 1021 ctgctgtttt ctgcatcttc atcatttgct tcggacccac aaacgtccTC CTGATTGCGC 1081 ATTACTCATT CCTTTCTCAC ACTTCCACCA CAGAGGCTGC CTACTTTGCC TACCTCCTCT 1141 GTGTCTGTGT CAGCAGCATA AGCTGCTGCA TCGACCCCCT AATTTACTAT TACGCTTCCT 1201 CTGAGTGCCA GAGGTACGTC TACAGTATCT TATGCTGCAA AGAAAGTTCC GATCCCAGCA 1261 GTTATAACAG CAGTGGGCAG TTGATGGCAA GTAAAATGGA TACCTGCTCT AGTAACCTGA 1321 ATAACAGCAT ATACAAAAAG CTGTTAACTG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1381 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1441 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAAAAT 1501 ATTATGTTCC TGCAGCCCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1561 tgaaagatt