Construct: ORF TRCN0000487846
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021164.1_s317c1
- DNA Barcode:
- GCACAGAACTCCCCGGGAGCTATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TSHR (7253)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487846
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7253 | TSHR | thyroid stimulating hormone... | NM_000369.3 | 100% | 100% | |
| 2 | human | 7253 | TSHR | thyroid stimulating hormone... | XM_005268037.4 | 100% | 100% | |
| 3 | human | 7253 | TSHR | thyroid stimulating hormone... | XM_011537119.2 | 87.4% | 85.1% | (many diffs) |
| 4 | human | 7253 | TSHR | thyroid stimulating hormone... | NM_001142626.3 | 34.4% | 32.3% | (many diffs) |
| 5 | human | 7253 | TSHR | thyroid stimulating hormone... | XM_006720245.1 | 34.4% | 32.3% | (many diffs) |
| 6 | human | 7253 | TSHR | thyroid stimulating hormone... | NM_001018036.3 | 32% | 30.1% | (many diffs) |
| 7 | human | 7253 | TSHR | thyroid stimulating hormone... | XM_005268039.1 | 32% | 30.1% | (many diffs) |
| 8 | mouse | 22095 | Tshr | thyroid stimulating hormone... | NM_011648.5 | 86.2% | 86.7% | (many diffs) |
| 9 | mouse | 22095 | Tshr | thyroid stimulating hormone... | NM_001113404.1 | 27.5% | 25.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2364
- ORF length:
- 2292
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaggccg gcggacttgc tgcagctggt gctgctgctc gacctgccca 121 gggacctggg cggaatgggg tgttcgtctc caccctgcga gtgccatcag gaggaggact 181 tcagagtcac ctgcaaggat attcaacgca tccccagctt accgcccagt acgcagactc 241 tgaagcttat tgagactcac ctgagaacta ttccaagtca tgcattttct aatctgccca 301 atatttccag aatctacgta tctatagatg tgactctgca gcagctggaa tcacactcct 361 tctacaattt gagtaaagtg actcacatag aaattcggaa taccaggaac ttaacttaca 421 tagaccctga tgccctcaaa gagctccccc tcctaaagtt ccttggcatt ttcaacactg 481 gacttaaaat gttccctgac ctgaccaaag tttattccac tgatatattc tttatacttg 541 aaattacaga caacccttac atgacgtcaa tccctgtgaa tgcttttcag ggactatgca 601 atgaaacctt gacactgaag ctgtacaaca atggctttac ttcagtccaa ggatatgctt 661 tcaatgggac aaagctggat gctgtttacc taaacaagaa taaatacctg acagttattg 721 acaaagatgc atttggagga gtatacagtg gaccaagctt gctggacgtg tctcaaacca 781 gtgtcactgc ccttccatcc aaaggcctgg agcacctgaa ggaactgata gcaagaaaca 841 cctggactct taagaaactt ccactttcct tgagtttcct tcacctcaca cgggctgacc 901 tttcttaccc aagccactgc tgtgctttta agaatcagaa gaaaatcaga ggaatccttg 961 agtccttgat gtgtaatgag agcagtatgc agagcttgcg ccagagaaaa tctgtgaatg 1021 ccttgaatag ccccctccac caggaatatg aagagaatct gggtgacagc attgttgggt 1081 acaaggaaaa gtccaagttc caggatactc ataacaacgc tcattattac gtcttctttg 1141 aagaacaaga ggatgagatc attggttttg gccaggagct caaaaacccc caggaagaga 1201 ctctacaagc ttttgacagc cattatgact acaccatatg tggggacagt gaagacatgg 1261 tgtgtacccc caagtccgat gagttcaacc cgtgtgaaga cataatgggc tacaagttcc 1321 tgagaattgt ggtgtggttc gttagtctgc tggctctcct gggcaatgtc tttgtcctgc 1381 ttattctcct caccagccac tacaaactga acgtcccccg ctttctcatg tgcaacctgg 1441 cctttgcgga tttctgcatg gggatgtacc tgctcctcat cgcctctgta gacctctaca 1501 ctcactctga gtactacaac catgccatcg actggcagac aggccctggg tgcaacacgg 1561 ctggtttctt cactgtcttt gcaagcgagt tatcggtgta tacgctgacg gtcatcaccc 1621 tggagcgctg gtatgccatc accttcgcca tgcgcctgga ccggaagatc cgcctcaggc 1681 acgcatgtgc catcatggtt gggggctggg tttgctgctt ccttctcgcc ctgcttcctt 1741 tggtgggaat aagtagctat gccaaagtca gtatctgcct gcccatggac accgagaccc 1801 ctcttgctct ggcatatatt gtttttgttc tgacgctcaa catagttgcc ttcgtcatcg 1861 tctgctgctg ttatgtgaag atctacatca cagtccgaaa tccgcagtac aacccagggg 1921 acaaagatac caaaattgcc aagaggatgg ctgtgttgat cttcaccgac ttcatatgca 1981 tggccccaat ctcattctat gctctgtcag caattctgaa caagcctctc atcactgtta 2041 gcaactccaa aatcttgctg gtactcttct atccacttaa ctcctgtgcc aatccattcc 2101 tctatgctat tttcaccaag gCCTTCCAGA GGGATGTGTT CATCCTACTC AGCAAGTTTG 2161 GCATCTGTAA ACGCCAGGCT CAGGCATACC GGGGGCAGAG GGTTCCTCCA AAGAACAGCA 2221 CTGATATTCA GGTTCAAAAG GTTACCCACG AGATGAGGCA GGGTCTCCAC AACATGGAAG 2281 ATGTCTATGA ACTGATTGAA AACTCCCATC TAACCCCAAA GAAGCAAGGC CAAATCTCAG 2341 AAGAGTATAT GCAAACGGTT TTGTAAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 2401 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2461 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCACAGAA 2521 CTCCCCGGGA GCTATCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2581 aagatt