Construct: ORF TRCN0000487851
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021485.1_s317c1
- DNA Barcode:
- GTAACGAGTTACCTGGTTAACTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NR3C2 (4306)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487851
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4306 | NR3C2 | nuclear receptor subfamily ... | NM_000901.5 | 100% | 100% | |
| 2 | human | 4306 | NR3C2 | nuclear receptor subfamily ... | XM_011531975.1 | 99.5% | 99.5% | 1898_1909del |
| 3 | human | 4306 | NR3C2 | nuclear receptor subfamily ... | NM_001166104.2 | 88.1% | 88.1% | 2012_2013ins351 |
| 4 | human | 4306 | NR3C2 | nuclear receptor subfamily ... | NM_001354819.1 | 88.1% | 88.1% | 2012_2013ins351 |
| 5 | human | 4306 | NR3C2 | nuclear receptor subfamily ... | XM_011531978.2 | 84.6% | 84.5% | (many diffs) |
| 6 | human | 4306 | NR3C2 | nuclear receptor subfamily ... | NR_148974.2 | 42.4% | 1_257del;2271_2272ins496;2714_5296del | |
| 7 | mouse | 110784 | Nr3c2 | nuclear receptor subfamily ... | NM_001083906.1 | 86.4% | 90.8% | (many diffs) |
| 8 | mouse | 110784 | Nr3c2 | nuclear receptor subfamily ... | XM_006530574.3 | 86.4% | 90.8% | (many diffs) |
| 9 | mouse | 110784 | Nr3c2 | nuclear receptor subfamily ... | XM_006530575.3 | 86.4% | 90.8% | (many diffs) |
| 10 | mouse | 110784 | Nr3c2 | nuclear receptor subfamily ... | XM_006530576.3 | 86.4% | 90.8% | (many diffs) |
| 11 | mouse | 110784 | Nr3c2 | nuclear receptor subfamily ... | XM_006530577.3 | 86.4% | 90.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 3027
- ORF length:
- 2952
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag accaaaggct accacagtct ccctgaaggt ctagatatgg 121 aaagacggtg gggtcaagtt tctcaggctg tggagcgttc ttccctggga cctacagaga 181 ggaccgatga gaataactac atggagattg tcaacgtaag ctgtgtttcc ggtgctattc 241 caaacaacag tactcaagga agcagcaaag aaaaacaaga actactccct tgccttcagc 301 aagacaataa tcggcctggg attttaacat ctgatattaa aactgagctg gaatctaagg 361 aactttcagc aactgtagct gagtccatgg gtttatatat ggattctgta agagatgctg 421 actattccta tgagcagcag aaccaacaag gaagcatgag tccagctaag atttatcaga 481 atgttgaaca gctggtgaaa ttttacaaag gaaatggcca tcgtccttcc actctaagtt 541 gtgtgaacac gcccttgaga tcatttatgt ctgactctgg gagctccgtg aatggtggcg 601 tcatgcgcgc cgttgttaaa agccctatca tgtgtcatga gaaaagcccg tctgtttgca 661 gccctctgaa catgacatct tcggtttgca gccctgctgg aatcaactct gtgtcctcca 721 ccacagccag ctttggcagt tttccagtgc acagcccaat cacccaggga actcctctga 781 catgctcccc taatgttgaa aatcgaggct ccaggtcgca cagccctgca catgctagca 841 atgtgggctc tcctctctca agtccgttaa gtagcatgaa atcctcaatt tccagccctc 901 caagtcactg cagtgtaaaa tctccagtct ccagtcccaa taatgtcact ctgagatcct 961 ctgtgtctag ccctgcaaat attaacaact caaggtgctc tgtttccagc ccttcgaaca 1021 ctaataacag atccacgctt tccagtccgg cagccagtac tgtgggatct atctgtagcc 1081 ctgtaaacaa tgccttcagc tacactgctt ctggcacctc tgctggatcc agtacattgc 1141 gggatgtggt tcccagtcca gacacgcagg agaaaggtgc tcaagaggtc ccttttccta 1201 agactgagga agtagagagt gccatctcaa atggtgtgac tggccagctt aatattgtcc 1261 agtacataaa accagaacca gatggagctt ttagcagctc atgtctagga ggaaatagca 1321 aaataaattc ggattcttca ttctcagtac caataaagca agaatcaacc aagcattcat 1381 gttcaggcac ctcttttaaa gggaatccaa cagtaaaccc gtttccattt atggatggct 1441 cgtatttttc ctttatggat gataaagact attattccct atcaggaatt ttaggaccac 1501 ctgtgcccgg ctttgatggt aactgtgaag gcagcggatt cccagtgggt attaaacaag 1561 aaccagatga tgggagctat tacccagagg ccagcatccc ttcctctgct attgttgggg 1621 tgaattcagg tggacagtcc ttccactaca ggattggtgc tcaaggtaca atatctttat 1681 cacgatcggc tagagaccaa tctttccaac acctgagttc ctttcctcct gtcaatactt 1741 tagtggagtc atggaaatca cacggcgacc tgtcgtctag aagaagtgat gggtatccgg 1801 tcttagaata cattccagaa aatgtatcaa gctctacttt acgaagtgtt tctactggat 1861 cttcaagacc ttcaaaaata tgtttggtgt gtggggatga ggcttcagga tgccattatg 1921 gggtagtcac ctgtggcagc tgcaaagttt tcttcaaaag agcagtggaa gggcaacaca 1981 actatttatg tgctggaaga aatgattgca tcattgataa gattcgacga aagaattgtc 2041 ctgcttgcag acttcagaaa tgtcttcaag ctggaatgaa tttaggagca cgaaagtcaa 2101 agaagttggg aaagttaaaa gggattcacg aggagcagcc acagcagcag cagcccccac 2161 ccccaccccc acccccgcaa agcccagagg aagggacaac gtacatcgct cctgcaaaag 2221 aaccctcggt caacacagca ctggttcctc agctctccac aatctcacga gcgctcacac 2281 cttcccccgt tatggtcctt gaaaacattg aacctgaaat tgtatatgca ggctatgaca 2341 gctcaaaacc agatacagcc gaaaatctgc tctccacgct caaccgctta gcaggcaaac 2401 agatgatcca agtcgtgaag tgggcaaagg tacttccagg atttaaaaac ttgcctcttg 2461 aggaccaaat taccctaatc cagtattctt ggatgtgtct atcatcattt gccttgagct 2521 ggagatcgta caaacatacg aacagccaat ttctctattt tgcaccagac ctagtcttta 2581 atgaagagaa gatgcatcag tctgccatgt atgaactatg ccaggggatg caccaaatca 2641 gccttcagtt cgttcgactg cagctcacct ttgaagaata caccatcatg aaagttttgc 2701 tgctactaag cacaattcca aaggatggcc tcaaaagcca ggctgcattt GAAGAAATGA 2761 GGACAAATTA CATCAAAGAA CTGAGGAAGA TGGTAACTAA GTGTCCCAAC AATTCTGGGC 2821 AGAGCTGGCA GAGGTTCTAC CAACTGACCA AGCTGCTGGA CTCCATGCAT GACCTGGTGA 2881 GCGACCTGCT GGAATTCTGC TTCTACACCT TCCGAGAGTC CCATGCGCTG AAGGTAGAGT 2941 TCCCCGCAAT GCTGGTGGAG ATCATCAGCG ACCAGCTGCC CAAGGTGGAG TCGGGGAACG 3001 CCAAGCCGCT CTACTTCCAC CGGAAGTGAT AGGACCCAGC TTTCTTGTAC AAAGTGGTTG 3061 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 3121 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT 3181 AACGAGTTAC CTGGTTAACT CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 3241 ttgtgaaaga tt