Construct: ORF TRCN0000487861
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021035.1_s317c1
- DNA Barcode:
- TCCATACTCTCCCACAGATCGTAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HCRTR2 (3062)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487861
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3062 | HCRTR2 | hypocretin receptor 2 | NM_001526.4 | 99.9% | 99.7% | 922A>G |
| 2 | human | 3062 | HCRTR2 | hypocretin receptor 2 | XM_017010798.1 | 96.2% | 96% | 922A>G;1333_1383del |
| 3 | mouse | 387285 | Hcrtr2 | hypocretin (orexin) receptor 2 | NM_198962.3 | 84.6% | 91.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1404
- ORF length:
- 1332
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtccggc accaaattgg aggactcccc cccttgtcgc aactggtcat 121 ctgcttcgga gctgaatgaa actcaagagc cctttttaaa ccccaccgac tatgacgacg 181 aggaattcct gcggtacctg tggagggaat acctgcaccc gaaagaatat gagtgggtcc 241 tgatcgccgg gtacatcatc gtgttcgtcg tggctctcat tgggaacgtc ctggtttgtg 301 tggcagtgtg gaagaaccac cacatgagga cggtaaccaa ctacttcata gtcaatcttt 361 ctctggctga tgtgctcgtg accatcacct gccttccagc cacactggtc gtggatatca 421 ctgagacctg gttttttgga cagtcccttt gcaaagtgat tccttatcta cagaccgtgt 481 cggtgtctgt gtctgtcctc acactgagct gtatcgcctt ggatcggtgg tatgcaatct 541 gtcacccttt gatgtttaag agcacagcaa agcgggcccg taacagcatt gtcatcatct 601 ggattgtctc ctgcattata atgattcctc aggccatcgt catggagtgc agcaccgtgt 661 tcccaggctt agccaataaa accaccctct ttacggtgtg tgatgagcgc tggggtggtg 721 aaatttatcc caagatgtac cacatctgtt tctttctggt gacatacatg gcaccactgt 781 gtctcatggt gttggcttat ctgcaaatat ttcgcaaact ctggtgtcga cagatccctg 841 gaacatcatc tgtagttcag agaaaatgga agcccctgca gcctgtttca cagcctcgag 901 ggccaggaca gccaacgaag tcccggatga gcgctgtggc ggctgaaata aagcagatcc 961 gagccagaag gaaaacagcc cggatgttga tggttgtgct tttggtattt gcaatttgct 1021 atctaccaat tagcatcctc aatgtgctaa agagagtatt tgggatgttt gcccatactg 1081 aagacagaga gactgtgtat gcctggttta ccttttcaca ctgGCTTGTA TATGCCAATA 1141 GTGCTGCGAA TCCAATTATT TATAATTTTC TCAGTGGAAA ATTTCGAGAG GAATTTAAAG 1201 CTGCGTTTTC TTGCTGTTGC CTTGGAGTTC ACCATCGCCA GGAGGATCGG CTCACCAGGG 1261 GACGAACTAG CACAGAGAGC CGGAAGTCCT TGACCACTCA AATCAGCAAC TTTGATAACA 1321 TATCAAAACT TTCTGAGCAA GTTGTGCTCA CTAGCATAAG CACACTCCCA GCAGCCAATG 1381 GAGCAGGACC ACTTCAAAAC TGGTAGAACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1441 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1501 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCCATACT 1561 CTCCCACAGA TCGTAGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1621 aagatt