Construct: ORF TRCN0000487864

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020298.1_s317c1
DNA Barcode:
TCCAGGACTTCTACTATTCCGACA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
CLK4 (57396)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487864
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57396 CLK4 CDC like kinase 4 NM_020666.3 99.7% 99.7% 1443_1444insTTG
2 mouse 12750 Clk4 CDC like kinase 4 NM_007714.6 90.4% 96.8% (many diffs)
3 mouse 12750 Clk4 CDC like kinase 4 XM_006532112.3 83.8% 86% (many diffs)
4 mouse 12750 Clk4 CDC like kinase 4 XM_006532113.3 83.8% 86% (many diffs)
5 mouse 12750 Clk4 CDC like kinase 4 XM_011248709.2 83.8% 86% (many diffs)
6 mouse 12750 Clk4 CDC like kinase 4 XM_006532111.3 82.8% 82.1% (many diffs)
7 mouse 12750 Clk4 CDC like kinase 4 XM_011248708.2 82.2% 83.3% (many diffs)
8 mouse 12750 Clk4 CDC like kinase 4 XM_017314243.1 78.7% 80.7% (many diffs)
9 mouse 12750 Clk4 CDC like kinase 4 NM_001304744.1 56.5% 61.4% (many diffs)
10 mouse 12750 Clk4 CDC like kinase 4 XM_006532114.3 56.5% 61.4% (many diffs)
11 mouse 12750 Clk4 CDC like kinase 4 XM_006532115.3 56.5% 61.4% (many diffs)
12 mouse 12750 Clk4 CDC like kinase 4 XM_006532116.2 56.5% 61.4% (many diffs)
13 mouse 12750 Clk4 CDC like kinase 4 XM_006532118.2 56.5% 61.4% (many diffs)
14 mouse 12750 Clk4 CDC like kinase 4 XM_017314244.1 56.5% 61.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1518
ORF length:
1446
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgcggcat tccaaaagaa ctcactgtcc tgattgggat agcagagaaa
121gctggggaca tgaaagctat cgtggaagtc acaagcggaa gaggagatct catagtagca
181cacaagagaa caggcattgt aaaccacatc accagtttaa agaatctgat tgtcattatt
241tagaagcaag gtccttgaat gagcgagatt atcgggaccg gagatacgtt gacgaataca
301ggaatgacta ctgtgaagga tatgttccta gacattatca cagagacatt gaaagcgggt
361atcgaatcca ctgcagtaaa tcttcagtcc gcagcaggag aagcagtcct aaaaggaagc
421gcaatagaca ctgttcaagt catcagtcac gttcgaagag ccaccgaagg aaaagatcca
481ggagtataga ggatgatgag gagggtcacc tgatctgtca aagtggagac gttctaagag
541caagatatga aatcgtggac actttgggtg aaggagcctt tggcaaagtt gtagagtgca
601ttgatcatgg catggatggc atgcatgtag cagtgaaaat cgtaaaaaat gtaggccgtt
661accgtgaagc agctcgttca gaaatccaag tattagagca cttaaatagt actgatccca
721atagtgtctt ccgatgtgtc cagatgctag aatggtttga tcatcatggt catgtttgta
781ttgtgtttga actactggga cttagtactt acgatttcat taaagaaaac agctttctgc
841catttcaaat tgaccacatc aggcagatgg cgtatcagat ctgccagtca ataaattttt
901tacatcataa taaattaacc catacagatc tgaagcctga aaatattttg tttgtgaagt
961ctgactatgt agtcaaatat aattctaaaa tgaaacgtga tgaacgcaca ctgaaaaaca
1021cagatatcaa agttgttgac tttggaagtg caacgtatga tgatgaacat cacagtactt
1081tggtgtctac ccggcactac agagctcccg aggtcatttt ggctttaggt tggtctcagc
1141cttgtgatgt ttggagcata ggttgcattc ttattgaata ttaccttggt ttcacagtct
1201ttcAGACTCA TGATAGTAAA GAGCACCTGG CAATGATGGA ACGAATATTA GGACCCATAC
1261CACAACACAT GATTCAGAAA ACAAGAAAAC GCAAGTATTT TCACCATAAC CAGCTAGATT
1321GGGATGAACA CAGTTCTGCT GGTAGATATG TTAGGAGACG CTGCAAACCG TTGAAGGAAT
1381TTATGCTTTG TCATGATGAA GAACATGAGA AACTGTTTGA CCTGGTTCGA AGAATGTTAG
1441AATATGATCC AACTCAAAGA ATTACCTTGG ATGAAGCATT GCAGCATCCT TTCTTTGACT
1501TATTAAAAAA GAAATTGTGA GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1561CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1621AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCCA GGACTTCTAC
1681TATTCCGACA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)