Construct: ORF TRCN0000487865
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019540.1_s317c1
- DNA Barcode:
- GATGTGGCTGGACCTGTTTAAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADCYAP1R1 (117)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487865
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 117 | ADCYAP1R1 | ADCYAP receptor type I | NM_001199635.1 | 99.9% | 99.7% | 1488_1489insG |
| 2 | human | 117 | ADCYAP1R1 | ADCYAP receptor type I | NM_001199636.2 | 99.7% | 99.5% | 1046_1047insCAG;1485_1486insG |
| 3 | human | 117 | ADCYAP1R1 | ADCYAP receptor type I | XM_017011736.2 | 98.5% | 98.3% | 264_265ins21;1467_1468insG |
| 4 | human | 117 | ADCYAP1R1 | ADCYAP receptor type I | XM_006715645.3 | 95.7% | 93.8% | (many diffs) |
| 5 | human | 117 | ADCYAP1R1 | ADCYAP receptor type I | XM_017011737.2 | 95.7% | 95.5% | 263_264ins63;1425_1426insG |
| 6 | human | 117 | ADCYAP1R1 | ADCYAP receptor type I | NM_001118.5 | 94.2% | 94.1% | 1045_1046ins84;1404_1405insG |
| 7 | human | 117 | ADCYAP1R1 | ADCYAP receptor type I | NM_001199637.2 | 90% | 89.9% | 263_264ins63;982_983ins84;1341_1342insG |
| 8 | human | 117 | ADCYAP1R1 | ADCYAP receptor type I | XM_017011738.2 | 88.4% | 88.3% | 154_155ins171;1317_1318insG |
| 9 | human | 117 | ADCYAP1R1 | ADCYAP receptor type I | XM_005249618.5 | 82.8% | 82.6% | 154_155ins171;874_875ins84;1233_1234insG |
| 10 | mouse | 11517 | Adcyap1r1 | adenylate cyclase activatin... | NM_007407.4 | 88.8% | 92.9% | (many diffs) |
| 11 | mouse | 11517 | Adcyap1r1 | adenylate cyclase activatin... | XM_011241152.2 | 88.6% | 92.7% | (many diffs) |
| 12 | mouse | 11517 | Adcyap1r1 | adenylate cyclase activatin... | XM_006505388.2 | 84.2% | 87.9% | (many diffs) |
| 13 | mouse | 11517 | Adcyap1r1 | adenylate cyclase activatin... | XM_011241150.1 | 84.2% | 87.9% | (many diffs) |
| 14 | mouse | 11517 | Adcyap1r1 | adenylate cyclase activatin... | NM_001025372.2 | 83.2% | 87.3% | (many diffs) |
| 15 | mouse | 11517 | Adcyap1r1 | adenylate cyclase activatin... | XM_011241151.1 | 81.2% | 85.3% | (many diffs) |
| 16 | mouse | 11517 | Adcyap1r1 | adenylate cyclase activatin... | XM_011241153.2 | 80% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1566
- ORF length:
- 1491
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggct ggtgtcgtgc acgtttccct ggctgctctc ctcctgctgc 121 ctatggcccc tgccatgcat tctgactgca tcttcaagaa ggagcaagcc atgtgcctgg 181 agaagatcca gagggccaat gagctgatgg gcttcaatga ttcctctcca ggctgtcctg 241 ggatgtggga caacatcacg tgttggaagc ccgcccatgt gggtgagatg gtcctggtca 301 gctgccctga gctcttccga atcttcaacc cagaccaagt ctgggagacc gaaaccattg 361 gagagtctga ttttggtgac agtaactcct tagatctctc agacatggga gtggtgagcc 421 ggaactgcac ggaggatggc tggtcggaac ccttccctca ttactttgat gcctgtgggt 481 ttgatgaata tgaatctgag actggggacc aggattatta ctacctgtca gtgaaggccc 541 tctacacggt tggctacagc acatccctcg tcaccctcac cactgccatg gtcatccttt 601 gtcgcttccg gaagctgcac tgcacacgca acttcatcca catgaacctg tttgtgtcgt 661 tcatgctgag ggcgatctcc gtcttcatca aagactggat tctgtatgcg gagcaggaca 721 gcaaccactg cttcatctcc actgtggaat gtaaggccgt catggttttc ttccactact 781 gtgttgtgtc caactacttc tggctgttca tcgagggcct gtacctcttc actctgctgg 841 tggagacctt cttccctgaa aggagatact tctactggta caccatcatt ggctggggga 901 ccccaactgt gtgtgtgaca gtgtgggcta cgctgagact ctactttgat gacacaggct 961 gctgggatat gaatgacagc acagctctgt ggtgggtgat caaaggccct gtggttggct 1021 ctatcatggt taactttgtg ctttttattg gcattatcgt catccttgtg cagaaacttc 1081 agtctccaga catgggaggc aatgagtcca gcatctactt cagctgcgtg cagaaatgct 1141 actgcaagcc acagcgggct cagcagcact cttgcaagat gtcagaactg tccaccatta 1201 ctctgcgact ggcccggtcc accctgctgc tcatcccact attcggaatc cactacacag 1261 tatttgCCTT CTCCCCAGAG AATGTCAGCA AAAGGGAAAG ACTCGTGTTT GAGCTGGGGC 1321 TGGGCTCCTT CCAGGGCTTT GTGGTGGCTG TTCTCTACTG TTTTCTGAAT GGTGAGGTAC 1381 AAGCGGAGAT CAAGCGAAAA TGGCGAAGCT GGAAGGTGAA CCGTTACTTC GCTGTGGACT 1441 TCAAGCACCG ACACCCGTCT CTGGCCAGCA GTGGGGTGAA TGGGGGCACC CAGCTCTCCA 1501 TCCTGAGCAA GAGCAGCTCC CAAATCCGCA TGTCTGGCCT CCCTGCTGAC AATCTGGCCA 1561 CCGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1621 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1681 GGCTTTATAT ATCTTGTGGA AAGGACGAGA TGTGGCTGGA CCTGTTTAAG ATACGCGTTA 1741 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt