Construct: ORF TRCN0000487872
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020533.1_s317c1
- DNA Barcode:
- GGGACCAACACCTTACTTGGGCGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GKAP1 (80318)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487872
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80318 | GKAP1 | G kinase anchoring protein 1 | NM_025211.4 | 100% | 100% | |
| 2 | human | 80318 | GKAP1 | G kinase anchoring protein 1 | XM_005252241.2 | 100% | 100% | |
| 3 | human | 80318 | GKAP1 | G kinase anchoring protein 1 | XM_011519058.2 | 100% | 100% | |
| 4 | human | 80318 | GKAP1 | G kinase anchoring protein 1 | NM_001135953.1 | 86% | 86% | 583_584ins153 |
| 5 | human | 80318 | GKAP1 | G kinase anchoring protein 1 | XM_006717293.1 | 66.1% | 66.1% | 0_1ins372 |
| 6 | human | 80318 | GKAP1 | G kinase anchoring protein 1 | XM_017015171.1 | 66.1% | 66.1% | 0_1ins372 |
| 7 | mouse | 56278 | Gkap1 | G kinase anchoring protein 1 | NM_019832.4 | 85.3% | 87.9% | (many diffs) |
| 8 | mouse | 56278 | Gkap1 | G kinase anchoring protein 1 | XM_006517301.3 | 85.3% | 87.9% | (many diffs) |
| 9 | mouse | 56278 | Gkap1 | G kinase anchoring protein 1 | XM_011244542.2 | 85.3% | 87.9% | (many diffs) |
| 10 | mouse | 56278 | Gkap1 | G kinase anchoring protein 1 | XM_011244543.2 | 85.3% | 87.9% | (many diffs) |
| 11 | mouse | 56278 | Gkap1 | G kinase anchoring protein 1 | XM_006517304.3 | 77.1% | 79.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1170
- ORF length:
- 1098
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcctca gcagtactta gttctgttcc caccaccgct tctcgttttg 121 ccctgttaca agtggatagt ggcagtggct ctgattctga acctggaaaa ggtaaaggtc 181 gaaatactgg aaagtctcaa actttaggaa gcaagtcaac tacaaatgag aaaaaaagag 241 agaaaagaag aaaaaagaag gaacagcaac agagtgaagc aaatgagctc aggaatcttg 301 cttttaagaa aattccccag aaatcctccc atgctgtttg taacgctcaa catgatcttc 361 cattgtcaaa cccagtacag aaggattcac gagaagaaaa ttggcaagag tggagacaaa 421 gagatgagca gctgacatct gaaatgtttg aagcagatct tgagaaggca ttgttactaa 481 gtaaactaga atatgaagag cacaaaaagg agtatgaaga tgctgaaaat acttcaactc 541 agtccaaagt tatgaataaa aaagataaaa gaaagaatca tcagggaaaa gacagacctc 601 tcacagtatc actaaaagat tttcattcgg aagatcacat tagtaaaaag actgaggaat 661 tgagttcttc tcagacttta tcacatgatg gaggattctt caatagactg gaagatgatg 721 ttcataaaat tcttattaga gaaaaacgaa gagaacagct tacagaatat aatggaacag 781 ataattgtac agctcatgaa cacaaccagg aagtggttct gaaagatgga agaattgaaa 841 gactaaagtt agagcttGAA AGGAAAGATG CTGAAATCCA GAAGCTGAAA AATGTAATCA 901 CTCAATGGGA GGCAAAGTAT AAGGAAGTAA AGGCAAGAAA TGCACAATTA TTGAAAATGC 961 TTCAGGAAGG TGAAATGAAA GATAAGGCAG AAATACTTCT GCAAGTTGAT GAATCACAAA 1021 GTATCAAGAA TGAGCTCACT ATTCAGGTGA CTTCACTTCA TGCTGCATTA GAACAAGAAA 1081 GATCTAAAGT GAAAGTATTA CAAGCAGAGT TAGCCAAATA CCAGGGTGGC AGAAAAGGGA 1141 AAAGAAACTC TGAATCCGAC CAGTGTAGGT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG 1201 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1261 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGG 1321 GACCAACACC TTACTTGGGC GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1381 ttgtgaaaga tt