Construct: ORF TRCN0000487886
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019565.1_s317c1
- DNA Barcode:
- CATTTCCCTCTCTTACTCCAATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTEN (5728)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487886
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5728 | PTEN | phosphatase and tensin homolog | NM_000314.8 | 100% | 100% | |
| 2 | human | 5728 | PTEN | phosphatase and tensin homolog | NM_001304717.5 | 69.9% | 69.9% | 1_519del |
| 3 | human | 5728 | PTEN | phosphatase and tensin homolog | NM_001304718.2 | 51.1% | 51.1% | 0_1ins591 |
| 4 | human | 11191 | PTENP1 | phosphatase and tensin homo... | NR_023917.1 | 30.2% | (many diffs) | |
| 5 | mouse | 19211 | Pten | phosphatase and tensin homolog | NM_008960.2 | 96.1% | 99.7% | (many diffs) |
| 6 | mouse | 19211 | Pten | phosphatase and tensin homolog | XM_006526769.2 | 63.8% | 66.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 1299
- ORF length:
- 1209
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgga tcctcagaca tgacagccat catcaaagag atcgttagca 121 gaaacaaaag gagatatcaa gaggatggat tcgacttaga cttgacctat atttatccaa 181 acattattgc tatgggattt cctgcagaaa gacttgaagg cgtatacagg aacaatattg 241 atgatgtagt aaggtttttg gattcaaagc ataaaaacca ttacaagata tacaatcttt 301 gtgctgaaag acattatgac accgccaaat ttaattgcag agttgcacaa tatccttttg 361 aagaccataa cccaccacag ctagaactta tcaaaccctt ttgtgaagat cttgaccaat 421 ggctaagtga agatgacaat catgttgcag caattcactg taaagctgga aagggacgaa 481 ctggtgtaat gatatgtgca tatttattac atcggggcaa atttttaaag gcacaagagg 541 ccctagattt ctatggggaa gtaaggacca gagacaaaaa gggagtaact attcccagtc 601 agaggcgcta tgtgtattat tatagctacc tgttaaagaa tcatctggat tatagaccag 661 tggcactgtt gtttcacaag atgatgtttg aaactattcc aatgttcagt ggcggaactt 721 gcaatcctca gtttgtggtc tgccagctaa aggtgaagat atattcctcc aattcaggac 781 ccacacgacg ggaagacaag ttcatgtact ttgagttccc tcagccgtta cctgtgtgtg 841 gtgatatcaa agtagagttc ttccacaaac agaacaagat gctaaaaaag gacaaaatgt 901 ttcacttttg ggtaaataca ttcttcatac caggaccaga ggaaacctca gaaaaagtag 961 aaaatggaag tctatgtgat caagaaatcg atagcatttG CAGTATAGAG CGTGCAGATA 1021 ATGACAAGGA ATATCTAGTA CTTACTTTAA CAAAAAATGA TCTTGACAAA GCAAATAAAG 1081 ACAAAGCCAA CCGATACTTT TCTCCAAATT TTAAGGTGAA GCTGTACTTC ACAAAAACAG 1141 TAGAGGAGCC GTCAAATCCA GAGGCTAGCA GTTCAACTTC TGTAACACCA GATGTTAGTG 1201 ACAATGAACC TGATCATTAT AGATATTCTG ACACCACTGA CTCTGATCCA GAGAATGAAC 1261 CTTTTGATGA AGATCAGCAT ACACAAATTA CAAAAGTCAA GGGTGGGCGC GCCGACCCAG 1321 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1381 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1441 TATCTTGTGG AAAGGACGAC ATTTCCCTCT CTTACTCCAA TTCACGCGTT AAGTCgacaa 1501 tcaacctctg gattacaaaa tttgtgaaag att