Construct: ORF TRCN0000487898
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021375.1_s317c1
- DNA Barcode:
- ATGACGTATACCCGCATGTTATTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PGC (5225)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487898
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5225 | PGC | progastricsin | NM_001166424.1 | 32.4% | 30.8% | (many diffs) |
| 2 | human | 5225 | PGC | progastricsin | NM_002630.4 | 26.3% | 25% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 384
- ORF length:
- 312
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaagtgg atggtggtgg tcttggtctg cctccagctc ttGGAGGCAG 121 CAGTGGTCAA AGTGCCCCTG AAGAAATTTA AGTCTATCCG TGAGACCATG AAGGAGAAGG 181 GCTTGCTGGG GGAGTTCCTG AGGACCCACA AGTATGATCC TGCTTGGAAG TACCGCTTTG 241 GTGACCTCAG CGTGACCTAC GAGCCCATGG CCTACATGGA TGCTGCCTAC TTTGGTGAGA 301 TCAGCATCGG GACTCCACCC CAGAACTTCC TGGTCCTTTT TGACACCGGC TCCTCCCCTC 361 TTCCTCTTGA CCCTGCACCC TCCTAGAACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 421 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 481 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATGACGTA 541 TACCCGCATG TTATTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 601 aagatt