Construct: ORF TRCN0000487900
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020672.1_s317c1
- DNA Barcode:
- CGAACGAGACCGACATCTCTTTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRKACG (5568)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487900
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5568 | PRKACG | protein kinase cAMP-activat... | NM_002732.3 | 100% | 100% | |
| 2 | human | 5566 | PRKACA | protein kinase cAMP-activat... | NM_002730.4 | 85.4% | 82.9% | (many diffs) |
| 3 | human | 5566 | PRKACA | protein kinase cAMP-activat... | NM_207518.3 | 83% | 80.6% | (many diffs) |
| 4 | human | 5566 | PRKACA | protein kinase cAMP-activat... | XM_017026948.1 | 72.7% | 70% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1125
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggcaac gcccccgcca agaaggacac cgagcaggag gagagcgtga 121 acgagttcct agccaaagcc agaggagatt tcctctacag atggggaaac cccgctcaaa 181 acaccgccag ctcggatcag ttcgaacggc tcaggacgct gggcatgggc tccttcgggc 241 gggtgatgct ggtgaggcac caggagaccg gcggccacta cgccatgaag atcctcaaca 301 agcagaaggt ggtgaagatg aagcaggtcg agcacatact gaacgagaag cgcatcctgc 361 aggcgatcga ctttccgttc ctcgtcaagc tccagttctc ctttaaggac aactcctacc 421 tgtacctggt gatggagtac gtgccgggtg gggagatgtt ctcccgccta cagcgcgtcg 481 gaaggtttag cgagccccat gcctgtttct atgccgccca ggtcgtcctg gccgtccagt 541 acctacactc gctcgacctc atccaccgcg acctgaagcc cgagaatctc ctcatcgacc 601 agcagggcta cctgcaggtg acggacttcg gtttcgccaa gcgcgtgaag ggccgcactt 661 ggaccttgtg cgggacccca gagtacctgg cccccgagat catcctgagc aaaggctaca 721 acaaggccgt ggactggtgg gccctagggg tgctcatcta tgagatggcc gtgggcttcc 781 cacccttcta cgccgaccag cccatccaga tctacgagaa gatcgtctct gggagggtgc 841 ggtttccctc caaactcagc tctgacctca agcatctgct gcggagcctg ctgcaggtgg 901 acctcaccaa gcgcttcgga aacctcagga acggggttgg cgacatCAAG AACCACAAGT 961 GGTTCGCCAC AACCAGCTGG ATCGCCATCT ATGAGAAGAA GGTGGAAGCT CCCTTCATCC 1021 CGAAGTACAC AGGCCCTGGG GATGCCAGTA ACTTTGACGA CTACGAGGAG GAAGAGCTCC 1081 GGATCTCCAT CAATGAGAAG TGTGCCAAGG AGTTTTCTGA GTTTTAGAAC CCAGCTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGACGAACGA GACCGACATC TCTTTTTACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt