Construct: ORF TRCN0000487902
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020117.1_s317c1
- DNA Barcode:
- TCATCCTTGACTCCCCAGTACCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP4K2 (5871)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487902
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5871 | MAP4K2 | mitogen-activated protein k... | NM_004579.5 | 99.9% | 99.7% | 134A>T;2460_2461insG |
| 2 | human | 5871 | MAP4K2 | mitogen-activated protein k... | NM_001307990.2 | 98.9% | 98.7% | 134A>T;1273_1274ins24;2436_2437insG |
| 3 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_024448629.1 | 95.2% | 95.1% | 134A>T;1167_1286del;2580_2581insG |
| 4 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_011545204.3 | 94.5% | 94.3% | 134A>T;529_530ins132;2328_2329insG |
| 5 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_017018093.2 | 93.5% | 93.4% | (many diffs) |
| 6 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_024448630.1 | 92.5% | 92.3% | (many diffs) |
| 7 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_024448631.1 | 90.1% | 90% | (many diffs) |
| 8 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_017018095.2 | 75.5% | 75.3% | 0_1ins531;1561_1562ins69;1860_1861insG |
| 9 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_024448633.1 | 74.7% | 74.6% | 0_1ins531;636_755del;2049_2050insG |
| 10 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XM_024448634.1 | 67.8% | 67.7% | 134A>T;1167_1286del;1872_1872delAins710 |
| 11 | human | 5871 | MAP4K2 | mitogen-activated protein k... | XR_002957155.1 | 46.2% | (many diffs) | |
| 12 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | NM_009006.3 | 87.7% | 93.5% | (many diffs) |
| 13 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_006531755.2 | 78.1% | 82.6% | (many diffs) |
| 14 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_006531756.2 | 78.1% | 82.6% | (many diffs) |
| 15 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_006531757.2 | 78.1% | 82.6% | (many diffs) |
| 16 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_017318211.1 | 78% | 83.4% | (many diffs) |
| 17 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_017318212.1 | 78% | 83.4% | (many diffs) |
| 18 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | NM_001291787.1 | 77.6% | 82.8% | (many diffs) |
| 19 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_006531760.3 | 77.6% | 82.8% | (many diffs) |
| 20 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XM_006531758.3 | 76.8% | 82.4% | (many diffs) |
| 21 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | XR_001782591.1 | 46% | (many diffs) | |
| 22 | mouse | 26412 | Map4k2 | mitogen-activated protein k... | NR_117093.1 | 45.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2532
- ORF length:
- 2463
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcgctgctg cgggatgtgt cgctgcagga cccgcgggac cgcttcgagc 121 tgctgcagcg cgtgggggcc gggacctatg gcgacgtcta caaggcccgc gacacggtca 181 cgtccgaact ggccgccgtg atgatagtca agctagaccc aggggacgac atcagctccc 241 tccagcagga aatcaccatc ctgcgtgagt gccgccaccc caatgtggtg gcctacattg 301 gcagctacct caggaatgac cgcttgtgga tctgcatgga gttctgcgga gggggctccc 361 tgcaggagat ttaccatgcc actgggcccc tggaggagcg gcagattgcc tacgtctgcc 421 gagaggcact gaaggggctc caccacctgc attctcaggg gaagatccac agagacatca 481 agggagccaa ccttctcctc actctccagg gagatgtcaa actggctgac tttggggtgt 541 caggcgagct gacagcgtct gtggccaaga ggaggtcttt cattgggact ccctactgga 601 tggctcccga ggtggctgct gtggagcgca aaggtggcta caatgagcta tgtgacgtct 661 gggccctggg catcactgcc attgagctgg gcgagctgca gccccctctg ttccacctgc 721 accccatgag ggccctgatg ctcatgtcga agagcagctt ccagccgccc aaactgagag 781 ataagactcg ctggacccag aatttccacc actttctcaa actggccctg accaagaatc 841 ctaagaagag gccgacagca gagaagctcc tgcagcaccc gttcacgact cagcagctcc 901 ctcgggccct cctcacacag ctgctggaca aagccagtga ccctcatctg gggaccccct 961 cccctgagga ctgtgagctg gagacctatg acatgtttcc agacaccatt cactcccggg 1021 ggcagcacgg cccagccgag aggaccccct cggagatcca gtttcaccag gtgaaatttg 1081 gcgccccacg caggaaggaa actgacccac tgaatgagcc gtgggaggaa gagtggacac 1141 tactgggaaa ggaagagttg agtgggagcc tgctgcagtc ggtccaggag gccctggagg 1201 aaaggagtct gactattcgg tcagcctcag aattccagga gctggactcc ccagacgata 1261 ccatgggaac catcaagcgg gccccgttcc tagggccact ccccactgac cctccagcag 1321 aggagcctct gtccagtccc ccaggaaccc tgcccccacc tccttcaggc cccaacagct 1381 ccccactgct gcccacggcc tgggccacca tgaagcagcg ggaggatcct gagaggtcat 1441 cctgccacgg gctcccccca actcccaagg tgcatatggg cgcctgcttc tccaaggtct 1501 tcaatggctg ccccctgcgg atccacgctg ctgtcacctg gattcaccct gttactcggg 1561 accagttcct ggtggtaggg gccgaggaag gcatctacac actcaacctg catgaactgc 1621 atgaggatac gctggagaag ctgatttcac atcgctgctc ctggctctac tgcgtgaaca 1681 acgtgctgct gtcactctca gggaaatcca cgcacatctg ggcccatgac ctcccaggcc 1741 tgtttgagca gcggaggcta cagcaacagg ttcccctctc catccccacc aaccgcctca 1801 cccagcgcat catccccagg cgctttgctc tgtccaccaa gattcctgac accaaaggct 1861 gcttgcagtg tcgtgtggtg cggaacccct acacgggtgc caccttcctg ctggccgccc 1921 tgcccaccag cctgctcctg ctgcagtggt atgagccgct gcagaagttt ctgctgctga 1981 agaacttctc cagccctctg cccagcccag ctgggatgct ggagccgctg gtgctggatg 2041 ggaaggagct gccgcaggtg tgtgttgggg ccgaggggcc tgaggggccc ggctgccgcg 2101 tcctgttcca tgtcctgccc ctggaggctg gcctgacgcc cgacatcctc atcccacctg 2161 aggggatccc aggctcggcc cagcaggtga tccaggtgga cagggacaca atcctagtca 2221 gctttgaacg ctgtgtgagg attgtcaaca tgcagggcga gcccacggcc acactggcac 2281 ctgagctgac ctttgatttc cccatcgaga ctgtggtgtg ccTGCAGGAC AGTGTGCTGG 2341 CCTTCTGGAG CCATGGGATG CAAGGCCGAA GCCTGGATAC CAATGAGGTG ACCCAGGAGA 2401 TCACAGATGA AACAAGGATC TTCCGAGTGC TTGGGGCCCA CAGAGACATC ATCCTGGAGA 2461 GCATTCCCAC TGACAACCCA GAGGCGCACA GCAACCTCTA CATCCTCACG GGCCACCAGA 2521 GCACCTACGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2581 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2641 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCATCC TTGACTCCCC AGTACCCGAC 2701 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt