Construct: ORF TRCN0000487905
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020765.1_s317c1
- DNA Barcode:
- GGGGTTCAGCCTTTAGCCCAACGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GPR183 (1880)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487905
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1880 | GPR183 | G protein-coupled receptor 183 | NM_004951.5 | 99.9% | 100% | 222G>T |
| 2 | human | 1880 | GPR183 | G protein-coupled receptor 183 | XM_017020405.2 | 99.9% | 100% | 222G>T |
| 3 | mouse | 321019 | Gpr183 | G protein-coupled receptor 183 | NM_183031.2 | 81.1% | 86.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1155
- ORF length:
- 1083
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatata caaatggcaa acaattttac tccgccctct gcaactcctc 121 agggaaatga ctgtgacctc tatgcacatc acagcacggc caggatagta atgcctctgc 181 attacagcct cgtcttcatc attgggctcg tgggaaactt actagccttg gtcgtcattg 241 ttcaaaacag gaaaaaaatc aactctacca ccctctattc aacaaatttg gttatttctg 301 atatactttt taccaccgct ttgcctacac gaatagccta ctatgcaatg ggctttgact 361 ggagaatcgg agatgccttg tgtaggataa ctgcgctagt gttttacatc aacacatatg 421 caggtgtgaa ctttatgacc tgcctgagta ttgaccgctt cattgctgtg gtgcaccctc 481 tacgctacaa caagataaaa aggattgaac atgcaaaagg cgtgtgcata tttgtctgga 541 ttctagtatt tgctcagaca ctcccactcc tcatcaaccc tatgtcaaag caggaggctg 601 aaaggattac atgcatggag tatccaaact ttgaagaaac taaatctctt ccctggattc 661 tgcttggggc atgtttcata ggatatgtac ttccacttat aatcattctc atctgctatt 721 ctcagatctg ctgcaaactc ttcagaactg ccaaacaaaa cccactcact gagaaatctg 781 gtgtaaacaa aaaggctctc aacacaatta ttcttattat tgttgtgttt gttctctgtt 841 tcacaCCTTA CCATGTTGCA ATTATTCAAC ATATGATTAA GAAGCTTCGT TTCTCTAATT 901 TCCTGGAATG TAGCCAAAGA CATTCGTTCC AGATTTCTCT GCACTTTACA GTATGCCTGA 961 TGAACTTCAA TTGCTGCATG GACCCTTTTA TCTACTTCTT TGCATGTAAA GGGTATAAGA 1021 GAAAGGTTAT GAGGATGCTG AAACGGCAAG TCAGTGTATC GATTTCTAGT GCTGTGAAGT 1081 CAGCCCCTGA AGAAAATTCA CGTGAAATGA CAGAAACGCA GATGATGATA CATTCCAAGT 1141 CTTCAAATGG AAAGTGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1201 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1261 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGGGTTC AGCCTTTAGC 1321 CCAACGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt