Construct: ORF TRCN0000487910

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021539.1_s317c1
DNA Barcode:
TGCAACTAGCCACCCCACGCAAGG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
ATP6V1C1 (528)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487910
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 528 ATP6V1C1 ATPase H+ transporting V1 s... NM_001695.5 100% 100%
2 mouse 66335 Atp6v1c1 ATPase, H+ transporting, ly... NM_025494.3 89.4% 98.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1218
ORF length:
1146
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgactgag ttctggctta tatctgctcc tggggagaaa acctgtcagc
121aaacatggga gaaattgcat gcggcaactt caaagaacaa taatcttgct gtcacttcca
181agttcaatat tcctgactta aaggttggca cgttggatgt cttggttggc ttgtcagatg
241aactggctaa actggatgca tttgtagaag gagtggttaa gaaagtagct caatacatgg
301ctgatgtatt ggaagatagc aaagacaaag ttcaagagaa tctgttggct aatggagtgg
361acttggttac ttatataaca aggttccagt gggacatggc caaatatcca atcaagcagt
421ccctgaaaaa tatttctgaa ataattgcca agggagtaac tcagattgat aatgacctga
481aatctcgagc atctgcatac aataacctga aaggaaatct tcagaatttg gaacgaaaga
541atgcaggaag tttgctaact agaagtctag cagaaattgt gaagaaggat gactttgttc
601ttgattcaga gtatctcgtc acattactgg tagtagttcc caagttaaac cacaacgact
661ggattaagca gtatgaaaca ctagccgaaa tggtagttcc aaggtctagc aatgttcttt
721cagaggacca agacagttac ctgtgtaatg tcaccttgtt taggaaggca gttgatgact
781tcagacacaa agccagagaa aacaaattca ttgttcgtga cttccagtat aatgaagagg
841agatgaaagc agataaagaa gaaatgaaca ggctttctac tgataagaaa aaacaatttg
901gaccacttgt acggtgGCTG AAAGTGAATT TTAGTGAAGC ATTTATTGCA TGGATTCACG
961TGAAAGCATT ACGGGTTTTC GTTGAGTCTG TTTTAAGGTA TGGCTTGCCA GTGAACTTCC
1021AAGCAATGCT ACTTCAGCCC AATAAGAAAA CTTTGAAGAA ACTGAGAGAA GTATTACATG
1081AATTGTATAA ACATCTAGAC AGCAGTGCAG CAGCTATTAT TGATGCTCCT ATGGATATTC
1141CAGGTTTAAA CCTGAGTCAA CAAGAATACT ACCCCTATGT GTACTACAAG ATTGATTGCA
1201ACTTGCTGGA ATTCAAGTAG AACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1261CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1321AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGCA ACTAGCCACC
1381CCACGCAAGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)