Construct: ORF TRCN0000487916
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019590.1_s317c1
- DNA Barcode:
- ATAACTACTAGACCTGACTGATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALNT1 (2589)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487916
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2589 | GALNT1 | polypeptide N-acetylgalacto... | NM_020474.3 | 99.9% | 99.8% | 1677_1678insG |
| 2 | mouse | 14423 | Galnt1 | UDP-N-acetyl-alpha-D-galact... | NM_001160404.1 | 93.5% | 98.2% | (many diffs) |
| 3 | mouse | 14423 | Galnt1 | UDP-N-acetyl-alpha-D-galact... | NM_013814.3 | 93.5% | 98.2% | (many diffs) |
| 4 | mouse | 14423 | Galnt1 | UDP-N-acetyl-alpha-D-galact... | XM_006525650.2 | 93.5% | 98.2% | (many diffs) |
| 5 | mouse | 14423 | Galnt1 | UDP-N-acetyl-alpha-D-galact... | XM_017317833.1 | 62.8% | 66.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1746
- ORF length:
- 1680
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat 61 tgaccatgag aaaatttgca tactgcaagg tggtcctagc cacctccttg atttgggtac 121 tcttggatat gttcctgctg ctttacttca gtgaatgcaa caaatgtgat gaaaaaaagg 181 agagaggact tcctgctgga gatgttctag agccagtaca aaagcctcat gaaggtcctg 241 gagaaatggg gaaaccagtc gtcattccta aagaggatca agaaaagatg aaagagatgt 301 ttaaaatcaa tcagttcaat ttaatggcaa gtgagatgat tgcactcaac agatctttac 361 cagatgttag gttagaaggg tgtaaaacaa aggtgtatcc agataatctt cctacaacaa 421 gtgtggtgat tgttttccac aatgaggctt ggagcacact tctgcgaact gtccatagtg 481 tcattaatcg ctcaccaaga cacatgatag aagaaattgt tctagtagat gatgccagtg 541 aaagagactt tttgaaaagg cctttagaga gttatgtgaa aaaactaaaa gtaccagttc 601 atgtaattcg aatggaacaa cgttctggat tgatcagagc tagattaaaa ggagctgctg 661 tgtctaaagg ccaagtgatc accttcctgg atgcccattg tgagtgtaca gtgggatggc 721 tggagcctct cttggccagg atcaaacatg acaggagaac agtggtgtgt cccatcatcg 781 atgtgatcag tgatgatact tttgagtaca tggcaggctc tgatatgacc tatggtgggt 841 tcaactggaa gctcaatttt cgctggtatc ctgttcccca aagagaaatg gacagaagga 901 aaggtgatcg gactcttcct gtcaggacac ctaccatggc aggaggcctt ttttcaatag 961 acagagatta ctttcaggaa attggaacat atgatgctgg aatggatatt tggggaggag 1021 aaaacctaga aatttccttt aggatttggc agtgtggagg aactttggaa attgttacat 1081 gctcacatgt tggacatgtg tttcggaaag ctacacctta cacgtttcca ggaggcacag 1141 ggcagattat caataaaaat aacagacgac ttgcagaagt gtggatggat gaattcaaga 1201 atttcttcta tataatttct ccaggtgtta caaaggtaga ttatggagat atatcgtcaa 1261 gagttggtct aagacacaaa ctacaatgca aacctttttc ctggtaccta gagaatatat 1321 atcctgattc tcaaattcca cgtcactatt tctcattggg agagatacga aatgtggaaa 1381 cgaatcagtg tctagataac atggctagaa aagagaatga aaaagttgga attttTAATT 1441 GCCATGGTAT GGGGGGTAAT CAGGTTTTCT CTTATACTGC CAACAAAGAA ATTAGAACAG 1501 ATGACCTTTG CTTGGATGTT TCCAAACTTA ATGGCCCAGT TACAATGCTC AAATGCCACC 1561 ACCTAAAAGG CAACCAACTC TGGGAGTATG ACCCAGTGAA ATTAACCCTG CAGCATGTGA 1621 ACAGTAATCA GTGCCTGGAT AAAGCCACAG AAGAGGATAG CCAGGTGCCC AGCATTAGAG 1681 ACTGCAATGG AAGTCGGTCC CAGCAGTGGC TTCTTCGAAA CGTCACCCTG CCAGAAATAT 1741 TCGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1801 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1861 GGCTTTATAT ATCTTGTGGA AAGGACGAAT AACTACTAGA CCTGACTGAT GAACGCGTTA 1921 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt