Construct: ORF TRCN0000487926
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021875.1_s317c1
- DNA Barcode:
- GCTTCGGCTGTTCTTATACGTTGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SYT17 (51760)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487926
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51760 | SYT17 | synaptotagmin 17 | NM_016524.4 | 99.9% | 100% | 612C>T |
| 2 | human | 51760 | SYT17 | synaptotagmin 17 | NM_001308157.2 | 99% | 98.9% | 0_1insATGGCGTAC;2_3insCCA;600C>T |
| 3 | human | 51760 | SYT17 | synaptotagmin 17 | XM_011545868.1 | 98.6% | 98.7% | 13_14insAGTTGGAACCATTAAACG;594C>T |
| 4 | human | 51760 | SYT17 | synaptotagmin 17 | XM_011545866.3 | 97.4% | 97.7% | 1_28del;33_33delGinsCGTACATC;633C>T |
| 5 | human | 51760 | SYT17 | synaptotagmin 17 | NM_001330509.1 | 87% | 87.1% | 0_1ins183;429C>T |
| 6 | human | 51760 | SYT17 | synaptotagmin 17 | XM_005255362.3 | 87% | 87.1% | 0_1ins183;429C>T |
| 7 | human | 51760 | SYT17 | synaptotagmin 17 | XM_011545869.2 | 87% | 87.1% | 0_1ins183;429C>T |
| 8 | human | 51760 | SYT17 | synaptotagmin 17 | XM_011545871.2 | 87% | 87.1% | 0_1ins183;429C>T |
| 9 | human | 51760 | SYT17 | synaptotagmin 17 | XM_011545872.2 | 87% | 87.1% | 0_1ins183;429C>T |
| 10 | human | 51760 | SYT17 | synaptotagmin 17 | XM_017023281.1 | 87% | 87.1% | 0_1ins183;429C>T |
| 11 | human | 51760 | SYT17 | synaptotagmin 17 | XM_024450297.1 | 87% | 87.1% | 0_1ins183;429C>T |
| 12 | human | 51760 | SYT17 | synaptotagmin 17 | XM_017023280.1 | 86.8% | 87% | (many diffs) |
| 13 | mouse | 110058 | Syt17 | synaptotagmin XVII | XM_006507204.3 | 88.3% | 96.2% | (many diffs) |
| 14 | mouse | 110058 | Syt17 | synaptotagmin XVII | NM_138649.1 | 87.4% | 95.1% | (many diffs) |
| 15 | mouse | 110058 | Syt17 | synaptotagmin XVII | XM_006507203.2 | 85.6% | 93.5% | (many diffs) |
| 16 | mouse | 110058 | Syt17 | synaptotagmin XVII | XM_006507205.3 | 77.2% | 84.5% | (many diffs) |
| 17 | mouse | 110058 | Syt17 | synaptotagmin XVII | XM_006507206.3 | 77.2% | 84.5% | (many diffs) |
| 18 | mouse | 110058 | Syt17 | synaptotagmin XVII | XM_006507202.2 | 71.7% | 77.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1491
- ORF length:
- 1422
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcgtacatc cagttggaac cattaaacga gggttttctt tctagaatct 121 ctggtctgct gctgtgcaga tggacctgcc ggcactgctg tcagaagtgc tacgagtcca 181 gctgttgcca gtcaagtgag gatgaagttg aaattctggg acctttccct gctcagaccc 241 ctccctggct gatggccagc cggagcagtg acaaggatgg tgactctgtc cacacggcca 301 gcgaagtccc gctgacccca cggaccaatt ccccggatgg aagacgctcg tcctcagaca 361 catccaagtc tacatacagc ctgacgcgga ggatttcgag tcttgagtca agacgtccca 421 gctctccact catcgatatt aaacccatcg agtttggcgt tctcagcgcc aagaaggagc 481 ccatccaacc ttcggtgctc agacggacct ataaccccga cgactatttc aggaagttcg 541 aaccccacct gtactccctc gactccaaca gcgacgatgt ggactctctg acagacgagg 601 agatcctgtc caagtaccag ctgggcatgc tgcacttcag cactcagtac gacctgctgc 661 acaaccacct caccgtgcgt gtgatcgagg ccagggacct gccacctccc atctcccacg 721 atggctcgcg ccaggacatg gcgcactcca acccctacgt caagatctgt ctcctgccag 781 accagaagaa ctcaaagcag accggggtca aacgcaagac ccagaagccc gtgtttgagg 841 agcgctacac cttcgagatc cccttcctgg aggcccagag gaggaccctg ctcctgaccg 901 tggtggattt tgataagttc tcccgccact gtgtcattgg gaaagtttct gtgcctttgt 961 gtgaagttga cctggtcaag ggcgggcact ggtggaaggc gctgattccc agttctcaga 1021 atgaagtgga gctgggggag ctgcttctgt cactgaatta tctcccaagt gctggcagac 1081 tgaatgttga tgtcattcga gccaagcaac ttcttcagac agatgtgagc caaggttcag 1141 acccctttgt gaaaatccag ctggtgcatg gactcaaact tgtgaaaacc aagaagacgt 1201 ccttcttaag gggcacaatt gatcctttct acaatgaaTC CTTCAGCTTC AAAGTTCCCC 1261 AAGAAGAACT GGAAAATGCC AGCCTAGTGT TTACAGTTTT CGGCCACAAC ATGAAGAGCA 1321 GCAATGACTT CATCGGGAGG ATCGTCATTG GCCAGTACTC TTCAGGCCCC TCTGAGACCA 1381 ACCACTGGAG GCGCATGCTC AACACGCACC GCACAGCCGT GGAGCAGTGG CATAGCCTGA 1441 GGTCCCGAGC TGAGTGTGAC CGCGTGTCTC CTGCCTCCCT GGAGGTGACC TAGAACCCAG 1501 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1561 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1621 TATCTTGTGG AAAGGACGAG CTTCGGCTGT TCTTATACGT TGGACGCGTT AAGTCgacaa 1681 tcaacctctg gattacaaaa tttgtgaaag att