Construct: ORF TRCN0000487949
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020146.1_s317c1
- DNA Barcode:
- TACCATGCTTCGTGATAAGCGCGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K3 (5606)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487949
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5606 | MAP2K3 | mitogen-activated protein k... | NM_001316332.2 | 99.7% | 99.3% | 879C>A;934G>A |
| 2 | human | 5606 | MAP2K3 | mitogen-activated protein k... | NM_002756.4 | 99.7% | 99.3% | 879C>A;934G>A |
| 3 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_005256723.2 | 99.7% | 99.3% | 879C>A;934G>A |
| 4 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_011523959.2 | 99.7% | 99.3% | 879C>A;934G>A |
| 5 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_017024858.1 | 99.7% | 99.3% | 879C>A;934G>A |
| 6 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_017024859.1 | 99.7% | 99.3% | 879C>A;934G>A |
| 7 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_017024857.2 | 92.5% | 92.1% | 1_75del;954C>A;1009G>A |
| 8 | human | 5606 | MAP2K3 | mitogen-activated protein k... | NM_145109.3 | 91.4% | 91% | 1_87del;966C>A;1021G>A |
| 9 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_011523958.2 | 78.5% | 71.7% | (many diffs) |
| 10 | mouse | 26397 | Map2k3 | mitogen-activated protein k... | NM_008928.4 | 83.1% | 88.7% | (many diffs) |
| 11 | mouse | 26397 | Map2k3 | mitogen-activated protein k... | XM_006533368.3 | 73.5% | 78.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1026
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtccaag ccacccgcac ccaaccccac acccccccgg aacctggact 121 cccggacctt catcaccatt ggagacagaa actttgaggt ggaggctgat gacttggtga 181 ccatctcaga actgggccgt ggagcctatg gggtggtaga gaaggtgcgg cacgcccaga 241 gcggcaccat catggccgtg aagcggatcc gggccaccgt gaactcacag gagcagaagc 301 ggctgctcat ggacctggac atcaacatgc gcacggtcga ctgtttctac actgtcacct 361 tctacggggc actattcaga gagggagacg tgtggatctg catggagctc atggacacat 421 ccttggacaa gttctaccgg aaggtgctgg ataaaaacat gacaattcca gaggacatcc 481 ttggggagat tgctgtgtct atcgtgcggg ccctggagca tctgcacagc aagctgtcgg 541 tgatccacag agatgtgaag ccctccaatg tccttatcaa caaggagggc catgtgaaga 601 tgtgtgactt tggcatcagt ggctacttgg tggactctgt ggccaagacg atggatgccg 661 gctgcaagcc ctacatggcc cctgagagga tcaacccaga gctgaaccag aagggctaca 721 atgtcaagtc cgacgtctgg agcctgggca tcaccatgat tgagatggcc atccTGCGGT 781 TCCCTTACGA GTCCTGGGGG ACCCCGTTCC AGCAGCTGAA GCAGGTGGTG GAGGAGCCGT 841 CCCCCCAGCT CCCAGCCGAC CGTTTCTCCC CCGAGTTTGT GGACTTCACT GCTCAGTGCC 901 TGAGGAAGAA CCCCGCAGAG CGTATGAGCT ACCTGGAGCT GATGGAGCAA CCCTTCTTCA 961 CCTTGCACAA AACCAAGAAG ACGGACATTG CTGCCTTCGT GAAGAAGATC CTGGGAGAAG 1021 ACTCATGAGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATACCAT GCTTCGTGAT AAGCGCGTAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt