Construct: ORF TRCN0000487951
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019886.1_s317c1
- DNA Barcode:
- CCATATCACGCACGTGGACTCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BMPR2 (659)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487951
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 659 | BMPR2 | bone morphogenetic protein ... | NM_001204.7 | 99.9% | 99.7% | 67A>G;1590C>A;3114_3115insG |
| 2 | human | 659 | BMPR2 | bone morphogenetic protein ... | XM_011511687.1 | 99.8% | 99.5% | (many diffs) |
| 3 | mouse | 12168 | Bmpr2 | bone morphogenetic protein ... | NM_007561.4 | 91.5% | 96.3% | (many diffs) |
| 4 | mouse | 12168 | Bmpr2 | bone morphogenetic protein ... | XM_006495633.1 | 91.4% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3189
- ORF length:
- 3117
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgacttcc tcgctgcagc ggccctggcg ggtgccctgg ctaccatgga 121 ccatcctgct ggtcagcgct gcggctgctt cgcagaatca agaacggcta tgtgcgttta 181 aagatccgta tcagcaagac cttgggatag gtgagagtag aatctctcat gaaaatggga 241 caatattatg ctcgaaaggt agcacctgct atggcctttg ggagaaatca aaaggggaca 301 taaatcttgt aaaacaagga tgttggtctc acattggaga tccccaagag tgtcactatg 361 aagaatgtgt agtaactacc actcctccct caattcagaa tggaacatac cgtttctgct 421 gttgtagcac agatttatgt aatgtcaact ttactgagaa ttttccacct cctgacacaa 481 caccactcag tccacctcat tcatttaacc gagatgagac aataatcatt gctttggcat 541 cagtctctgt attagctgtt ttgatagttg ccttatgctt tggatacaga atgttgacag 601 gagaccgtaa acaaggtctt cacagtatga acatgatgga ggcagcagca tccgaaccct 661 ctcttgatct agataatctg aaactgttgg agctgattgg ccgaggtcga tatggagcag 721 tatataaagg ctccttggat gagcgtccag ttgctgtaaa agtgttttcc tttgcaaacc 781 gtcagaattt tatcaacgaa aagaacattt acagagtgcc tttgatggaa catgacaaca 841 ttgcccgctt tatagttgga gatgagagag tcactgcaga tggacgcatg gaatatttgc 901 ttgtgatgga gtactatccc aatggatctt tatgcaagta tttaagtctc cacacaagtg 961 actgggtaag ctcttgccgt cttgctcatt ctgttactag aggactggct tatcttcaca 1021 cagaattacc acgaggagat cattataaac ctgcaatttc ccatcgagat ttaaacagca 1081 gaaatgtcct agtgaaaaat gatggaacct gtgttattag tgactttgga ctgtccatga 1141 ggctgactgg aaatagactg gtgcgcccag gggaggaaga taatgcagcc ataagcgagg 1201 ttggcactat cagatatatg gcaccagaag tgctagaagg agctgtgaac ttgagggact 1261 gtgaatcagc tttgaaacaa gtagacatgt atgctcttgg actaatctat tgggagatat 1321 ttatgagatg tacagacctc ttcccagggg aatccgtacc agagtaccag atggcttttc 1381 agacagaggt tggaaaccat cccacttttg aggatatgca ggttctcgtg tctagggaaa 1441 aacagagacc caagttccca gaagcctgga aagaaaatag cctggcagtg aggtcactca 1501 aggagacaat cgaagactgt tgggaccagg atgcagaggc tcggcttact gcacagtgtg 1561 ctgaggaaag gatggctgaa cttatgatga tttgggaaag aaacaaatct gtgagcccaa 1621 cagtcaatcc aatgtctact gctatgcaga atgaacgcaa actgtcacat aataggcgtg 1681 tgccaaaaat tggtccttat ccagattatt cttcctcctc atacattgaa gactctatcc 1741 atcatactga cagcatcgtg aagaatattt cctctgagca ttctatgtcc agcacacctt 1801 tgactatagg ggaaaaaaac cgaaattcaa ttaactatga acgacagcaa gcacaagctc 1861 gaatccccag ccctgaaaca agtgtcacca gcctctccac caacacaaca accacaaaca 1921 ccacaggact cacgccaagt actggcatga ctactatatc tgagatgcca tacccagatg 1981 aaacaaatct gcataccaca aatgttgcac agtcaattgg gccaacccct gtctgcttac 2041 agctgacaga agaagacttg gaaaccaaca agctagaccc aaaagaagtt gataagaacc 2101 tcaaggaaag ctctgatgag aatctcatgg agcactctct taaacagttc agtggcccag 2161 acccactgag cagtactagt tctagcttgc tttacccact cataaaactt gcagtagaag 2221 caactggaca gcaggacttc acacagactg caaatggcca agcatgtttg attcctgatg 2281 ttctgcctac tcagatctat cctctcccca agcagcagaa ccttcccaag agacctacta 2341 gtttgccttt gaacaccaaa aattcaacaa aagagccccg gctaaaattt ggcagcaagc 2401 acaaatcaaa cttgaaacaa gtcgaaactg gagttgccaa gatgaataca atcaatgcag 2461 cagaacctca tgtggtgaca gtcaccatga atggtgtggc aggtagaaac cacagtgtta 2521 actcccatgc tgccacaacc caatatgcca atgggacagt actatctggc caaacaacca 2581 acatagtgac acatagggcc caagaaatgt tgcagaatca gtttattggt gaggacaccc 2641 ggctgaatat taattccagt cctgatgagc atgagccttt actgagacga gagcaacaag 2701 ctggccatga tgaaggtgtt ctggatcgtc ttgtggacag gagggaacgg ccactagaag 2761 gtggccgaac taattccaat aacaacaaca gcaatccatg ttcagaacaa gatgttcttg 2821 cacagggtgt tccaagcaca gcagcagatc ctgggccatc aaagcccaga agagcacaga 2881 ggcctaattc tctggatctt tcagccacaa atgtcctgga tggcagcagt atacagatag 2941 gtgagtcaac acaagatggc aaatcaggat CAGGTGAAAA GATCAAGAAA CGTGTGAAAA 3001 CTCCCTATTC TCTTAAGCGG TGGCGCCCCT CCACCTGGGT CATCTCCACT GAATCGCTGG 3061 ACTGTGAAGT CAACAATAAT GGCAGTAACA GGGCAGTTCA TTCCAAATCC AGCACTGCTG 3121 TTTACCTTGC AGAAGGAGGC ACTGCTACAA CCATGGTGTC TAAAGATATA GGAATGAACT 3181 GTCTGGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 3241 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 3301 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCATATCAC GCACGTGGAC TCCCTACGCG 3361 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt