Construct: ORF TRCN0000487958
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020650.1_s317c1
- DNA Barcode:
- CAGGCTACCAGGCTAAGTTACTGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- INSR (3643)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487958
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3643 | INSR | insulin receptor | NM_001079817.3 | 99.9% | 99.9% | 5C>G;3219C>T |
| 2 | human | 3643 | INSR | insulin receptor | XM_011527989.3 | 99.8% | 99.7% | 5C>G;2806_2807insTAG;3216C>T |
| 3 | human | 3643 | INSR | insulin receptor | NM_000208.4 | 99% | 99% | 5C>G;2230_2265del;3255C>T |
| 4 | human | 3643 | INSR | insulin receptor | XM_011527988.2 | 99% | 98.9% | (many diffs) |
| 5 | mouse | 16337 | Insr | insulin receptor | NM_010568.3 | 87% | 95.2% | (many diffs) |
| 6 | mouse | 16337 | Insr | insulin receptor | XM_006508701.1 | 86.9% | 95.1% | (many diffs) |
| 7 | mouse | 16337 | Insr | insulin receptor | NM_001330056.1 | 86.2% | 94.4% | (many diffs) |
| 8 | mouse | 16337 | Insr | insulin receptor | XM_006508700.1 | 86.1% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 4194
- ORF length:
- 4110
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatgggca ccgggggccg gcggggggcg gcggccgcgc 121 cgctgctggt ggcggtggcc gcgctgctac tgggcgccgc gggccacctg taccccggag 181 aggtgtgtcc cggcatggat atccggaaca acctcactag gttgcatgag ctggagaatt 241 gctctgtcat cgaaggacac ttgcagatac tcttgatgtt caaaacgagg cccgaagatt 301 tccgagacct cagtttcccc aaactcatca tgatcactga ttacttgctg ctcttccggg 361 tctatgggct cgagagcctg aaggacctgt tccccaacct cacggtcatc cggggatcac 421 gactgttctt taactacgcg ctggtcatct tcgagatggt tcacctcaag gaactcggcc 481 tctacaacct gatgaacatc acccggggtt ctgtccgcat cgagaagaac aatgagctct 541 gttacttggc cactatcgac tggtcccgta tcctggattc cgtggaggat aattacatcg 601 tgttgaacaa agatgacaac gaggagtgtg gagacatctg tccgggtacc gcgaagggca 661 agaccaactg ccccgccacc gtcatcaacg ggcagtttgt cgaacgatgt tggactcata 721 gtcactgcca gaaagtttgc ccgaccatct gtaagtcaca cggctgcacc gccgaaggcc 781 tctgttgcca cagcgagtgc ctgggcaact gttctcagcc cgacgacccc accaagtgcg 841 tggcctgccg caacttctac ctggacggca ggtgtgtgga gacctgcccg cccccgtact 901 accacttcca ggactggcgc tgtgtgaact tcagcttctg ccaggacctg caccacaaat 961 gcaagaactc gcggaggcag ggctgccacc agtacgtcat tcacaacaac aagtgcatcc 1021 ctgagtgtcc ctccgggtac acgatgaatt ccagcaactt gctgtgcacc ccatgcctgg 1081 gtccctgtcc caaggtgtgc cacctcctag aaggcgagaa gaccatcgac tcggtgacgt 1141 ctgcccagga gctccgagga tgcaccgtca tcaacgggag tctgatcatc aacattcgag 1201 gaggcaacaa tctggcagct gagctagaag ccaacctcgg cctcattgaa gaaatttcag 1261 ggtatctaaa aatccgccga tcctacgctc tggtgtcact ttccttcttc cggaagttac 1321 gtctgattcg aggagagacc ttggaaattg ggaactactc cttctatgcc ttggacaacc 1381 agaacctaag gcagctctgg gactggagca aacacaacct caccatcact caggggaaac 1441 tcttcttcca ctataacccc aaactctgct tgtcagaaat ccacaagatg gaagaagttt 1501 caggaaccaa ggggcgccag gagagaaacg acattgccct gaagaccaat ggggaccagg 1561 catcctgtga aaatgagtta cttaaatttt cttacattcg gacatctttt gacaagatct 1621 tgctgagatg ggagccgtac tggccccccg acttccgaga cctcttgggg ttcatgctgt 1681 tctacaaaga ggccccttat cagaatgtga cggagttcga cgggcaggat gcgtgtggtt 1741 ccaacagttg gacggtggta gacattgacc cacccctgag gtccaacgac cccaaatcac 1801 agaaccaccc agggtggctg atgcggggtc tcaagccctg gacccagtat gccatctttg 1861 tgaagaccct ggtcaccttt tcggatgaac gccggaccta tggggccaag agtgacatca 1921 tttatgtcca gacagatgcc accaacccct ctgtgcccct ggatccaatc tcagtgtcta 1981 actcatcatc ccagattatt ctgaagtgga aaccaccctc cgaccccaat ggcaacatca 2041 cccactacct ggttttctgg gagaggcagg cggaagacag tgagctgttc gagctggatt 2101 attgcctcaa agggctgaag ctgccctcga ggacctggtc tccaccattc gagtctgaag 2161 attctcagaa gcacaaccag agtgagtatg aggattcggc cggcgaatgc tgctcctgtc 2221 caaagacaga ctctcagatc ctgaaggagc tggaggagtc ctcgtttagg aagacgtttg 2281 aggattacct gcacaacgtg gttttcgtcc ccaggccatc tcggaaacgc aggtcccttg 2341 gcgatgttgg gaatgtgacg gtggccgtgc ccacggtggc agctttcccc aacacttcct 2401 cgaccagcgt gcccacgagt ccggaggagc acaggccttt tgagaaggtg gtgaacaagg 2461 agtcgctggt catctccggc ttgcgacact tcacgggcta tcgcatcgag ctgcaggctt 2521 gcaaccagga cacccctgag gaacggtgca gtgtggcagc ctacgtcagt gcgaggacca 2581 tgcctgaagc caaggctgat gacattgttg gccctgtgac gcatgaaatc tttgagaaca 2641 acgtcgtcca cttgatgtgg caggagccga aggagcccaa tggtctgatc gtgctgtatg 2701 aagtgagtta tcggcgatat ggtgatgagg agctgcatct ctgcgtctcc cgcaagcact 2761 tcgctctgga acggggctgc aggctgcgtg ggctgtcacc ggggaactac agcgtgcgaa 2821 tccgggccac ctcccttgcg ggcaacggct cttggacgga acccacctat ttctacgtga 2881 cagactattt agacgtcccg tcaaatattg caaaaattat catcggcccc ctcatctttg 2941 tctttctctt cagtgttgtg attggaagta tttatctatt cctgagaaag aggcagccag 3001 atgggccgct gggaccgctt tacgcttctt caaaccctga gtatctcagt gccagtgatg 3061 tgtttccatg ctctgtgtac gtgccggacg agtgggaggt gtctcgagag aagatcaccc 3121 tccttcgaga gctggggcag ggctccttcg gcatggtgta tgagggcaat gccagggaca 3181 tcatcaaggg tgaggcagag acccgcgtgg cggtgaagac ggtcaacgag tcagccagtc 3241 tccgagagcg gattgagttc ctcaatgagg cctcggtcat gaagggcttc acctgccatc 3301 atgtggtgcg cctcctggga gtggtgtcca agggccagcc cacgctggtg gtgatggagc 3361 tgatggctca cggagacctg aagagctacc tccgttctct gcggccagag gctgagaata 3421 atcctggccg ccctccccct acccttcaag agatgattca gatggcggca gagattgctg 3481 acgggatggc ctacctgaac gccaagaagt ttgtgcatcg ggacctggca gcgagaaact 3541 gcatggtcgc ccatgatttt actgtcaaaa ttggagactt tggaatgacc agagacatct 3601 atgaaacgga ttactaccgg aaagggggca agggtctgct ccctgtacgg tggatggcac 3661 cggagtccct gaaggatggg gtcttcacca cttcttctga catgtggtcc tttggcgtgg 3721 tcctttggga aatcaccagc ttggcagaac agccttacca aggcctgtct aatgaacagg 3781 tgttgaaatt tgtcatggat ggagggtatc tggatcaacc cgacaactgt ccagagagag 3841 tcactgacct catgcgcatg tgctggcaat tcaaccccaa gatgaggcca accttcctgg 3901 agattgtcaa ccTGCTCAAG GACGACCTGC ACCCCAGCTT TCCAGAGGTG TCGTTCTTCC 3961 ACAGCGAGGA GAACAAGGCT CCCGAGAGTG AGGAGCTGGA GATGGAGTTT GAGGACATGG 4021 AGAATGTGCC CCTGGACCGT TCCTCGCACT GTCAGAGGGA GGAGGCGGGG GGCCGGGATG 4081 GAGGGTCCTC GCTGGGTTTC AAGCGGAGCT ACGAGGAACA CATCCCTTAC ACACACATGA 4141 ACGGAGGCAA GAAAAACGGG CGGATTCTGA CCTTGCCTCG GTCCAATCCT TCCTAAAAGG 4201 GTGGGCGCGC CGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 4261 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 4321 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAG GCTACCAGGC TAAGTTACTG 4381 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t