Transcript: Human XM_011527989.3

PREDICTED: Homo sapiens insulin receptor (INSR), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
INSR (3643)
Length:
9423
CDS:
515..4624

Additional Resources:

NCBI RefSeq record:
XM_011527989.3
NBCI Gene record:
INSR (3643)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149243 AGTGAGTATGAGGATTCGGC pXPR_003 CGG 2114 51% 10 0.5658 INSR INSR 75508
2 BRDN0001145804 TTATCGGCGATATGGTGATG pXPR_003 AGG 2641 64% 12 0.3431 INSR INSR 75506
3 BRDN0001148089 TGTTGTGAATGACGTACTGG pXPR_003 TGG 908 22% 3 -0.1802 INSR INSR 75507
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011527989.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000121282 CTCGTTTGGTTACCAATTTAA pLKO.1 4777 3UTR 100% 15.000 21.000 N INSR n/a
2 TRCN0000121219 GAAGTGAGTTATCGGCGATAT pLKO.1 3131 CDS 100% 10.800 15.120 N INSR n/a
3 TRCN0000121124 CCACCATTCGAGTCTGAAGAT pLKO.1 2573 CDS 100% 4.950 6.930 N INSR n/a
4 TRCN0000121123 CGTGTTGAACAAAGATGACAA pLKO.1 1030 CDS 100% 4.950 6.930 N INSR n/a
5 TRCN0000121221 AGAGACATCTATGAAACGGAT pLKO.1 4019 CDS 100% 2.640 2.112 N INSR n/a
6 TRCN0000039700 GCCAAGAGTGACATCATTTAT pLKO.1 2336 CDS 100% 15.000 10.500 N INSR n/a
7 TRCN0000196786 GAAACTCTTCTTCCACTATAA pLKO.1 1867 CDS 100% 13.200 9.240 N INSR n/a
8 TRCN0000000380 GTGCTGTATGAAGTGAGTTAT pLKO.1 3122 CDS 100% 13.200 9.240 N INSR n/a
9 TRCN0000000382 CAGTGTTGTGATTGGAAGTAT pLKO.1 3379 CDS 100% 5.625 3.938 N INSR n/a
10 TRCN0000039701 CCTACCCTTCAAGAGATGATT pLKO.1 3866 CDS 100% 5.625 3.938 N INSR n/a
11 TRCN0000199622 GCGCATGTGCTGGCAATTCAA pLKO.1 4282 CDS 100% 5.625 3.938 N INSR n/a
12 TRCN0000121125 CAGATTATTCTGAAGTGGAAA pLKO.1 2423 CDS 100% 4.950 3.465 N INSR n/a
13 TRCN0000194847 CCTATACATTTCTGTTCATCT pLKO.1 4747 3UTR 100% 4.950 3.465 N INSR n/a
14 TRCN0000195635 CGAAGGACACTTGCAGATACT pLKO.1 682 CDS 100% 4.950 3.465 N INSR n/a
15 TRCN0000121220 GAAATCCACAAGATGGAAGAA pLKO.1 1907 CDS 100% 4.950 3.465 N INSR n/a
16 TRCN0000121218 GAGCTGGATTATTGCCTCAAA pLKO.1 2522 CDS 100% 4.950 3.465 N INSR n/a
17 TRCN0000009843 GAGTTCAGAGATCGTTCCTAT pLKO.1 4731 3UTR 100% 4.950 3.465 N IRS1 n/a
18 TRCN0000000379 GCTCTGTTACTTGGCCACTAT pLKO.1 967 CDS 100% 4.950 3.465 N INSR n/a
19 TRCN0000039699 GCTGGAGAATTGCTCTGTCAT pLKO.1 661 CDS 100% 4.950 3.465 N INSR n/a
20 TRCN0000039698 ACCTATTTCTACGTGACAGAC pLKO.1 3296 CDS 100% 4.050 2.835 N INSR n/a
21 TRCN0000000381 CACTGATTACTTGCTGCTCTT pLKO.1 766 CDS 100% 4.050 2.835 N INSR n/a
22 TRCN0000121286 GTGTTGAAATTTGTCATGGAT pLKO.1 4208 CDS 100% 3.000 2.100 N INSR n/a
23 TRCN0000018332 CATGGATATCCGGAACAACCT pLKO.1 625 CDS 100% 2.640 1.848 N INSR n/a
24 TRCN0000121126 GAGCGGATTGAGTTCCTCAAT pLKO.1 3674 CDS 100% 0.495 0.347 N INSR n/a
25 TRCN0000039702 CCTGTCTAATGAACAGGTGTT pLKO.1 4192 CDS 100% 0.405 0.284 N INSR n/a
26 TRCN0000195618 CCTTACCAAGGCCTGTCTAAT pLKO.1 4181 CDS 100% 13.200 7.920 N INSR n/a
27 TRCN0000010523 CTCAGGATTCTCACGACTCTA pLKO.1 4698 3UTR 100% 4.950 2.970 N INSR n/a
28 TRCN0000121283 CCTTTGGGAAATCACCAGCTT pLKO.1 4150 CDS 100% 2.640 1.584 N INSR n/a
29 TRCN0000121217 CTGGTTTGAAAGCCTCTGGAA pLKO.1 4675 3UTR 100% 2.640 1.584 N INSR n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011527989.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489414 ACATAGAATGGAGATTGAGGCCAA pLX_317 8.8% 99.8% 99.7% V5 5C>G;2806_2807insTAG;3216C>T n/a
2 TRCN0000487958 CAGGCTACCAGGCTAAGTTACTGC pLX_317 7.4% 99.8% 99.7% V5 (not translated due to prior stop codon) 5C>G;2806_2807insTAG;3216C>T n/a
3 TRCN0000489232 CCATAATTGTCAGCCTTGTTAGTT pLX_317 1.3% 99% 98.9% V5 5C>G;2229_2230ins36;2806_2807insTAG n/a
4 TRCN0000491410 ACTATATTCATACGTACTTCCCGA pLX_317 8.7% 99% 98.9% V5 (not translated due to prior stop codon) 5C>G;2229_2230ins36;2806_2807insTAG n/a
5 TRCN0000487917 TTCGCGCTATCTACGTCATTTCTA pLX_317 26.8% 27.6% 1% V5 (not translated due to prior stop codon) 1_2971del n/a
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