Construct: ORF TRCN0000487959
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020525.1_s317c1
- DNA Barcode:
- CGACCGATTATTCACTTTGCAAAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PANK1 (53354)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487959
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_017016338.1 | 100% | 100% | |
| 2 | human | 53354 | PANK1 | pantothenate kinase 1 | NM_138316.4 | 42.1% | 33.3% | (many diffs) |
| 3 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_017016337.1 | 38.2% | 38.2% | 1_663del |
| 4 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_017016336.1 | 37.1% | 37.1% | 1_696del |
| 5 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_017016335.1 | 36.9% | 36.9% | 1_702del |
| 6 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_024448040.1 | 36.9% | 36.9% | 1_702del |
| 7 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_024448041.1 | 36.9% | 36.9% | 1_702del |
| 8 | human | 53354 | PANK1 | pantothenate kinase 1 | NM_148978.3 | 36.7% | 36.7% | 1_708del |
| 9 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_017016334.1 | 35.3% | 35.3% | 1_753del |
| 10 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_017016333.2 | 34.2% | 34.2% | 1_789del |
| 11 | human | 53354 | PANK1 | pantothenate kinase 1 | NM_148977.2 | 22.9% | 22.9% | 1_1383del |
| 12 | mouse | 75735 | Pank1 | pantothenate kinase 1 | NM_023792.2 | 34.4% | 35.9% | (many diffs) |
| 13 | mouse | 75735 | Pank1 | pantothenate kinase 1 | XM_006527427.3 | 25.4% | 21% | (many diffs) |
| 14 | mouse | 75735 | Pank1 | pantothenate kinase 1 | NM_001114339.2 | 23.4% | 24.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 483
- ORF length:
- 411
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcagct aaaggcgaca gcaccaatgt tgataaactg gtgaaggaca 121 tttacggagg agactatgaa cgatttggcc ttcaaggatc tgctgtagca tcaagctttG 181 GCAACATGAT GAGTAAAGAA AAGCGAGATT CCATCAGCAA GGAAGACCTC GCCCGGGCCA 241 CATTGGTCAC CATCACCAAC AACATTGGCT CCATTGCTCG GATGTGCGCG TTGAATGAGA 301 ACATAGACAG AGTTGTGTTT GTTGGAAATT TTCTCAGAAT CAATATGGTC TCCATGAAGC 361 TGCTGGCATA TGCCATGGAT TTTTGGTCCA AAGGACAACT GAAAGCTCTG TTTTTGGAAC 421 ATGAGGGTTA TTTTGGAGCC GTTGGGGCAC TGTTGGAACT GTTCAAAATG ACTGATGACA 481 AGTAGAACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 541 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 601 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACGACCGATT ATTCACTTTG CAAAAACGCG 661 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt