Construct: ORF TRCN0000487961
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020027.1_s317c1
- DNA Barcode:
- ATCGGCGAGAATGGCTCGTAACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAMK2N2 (94032)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487961
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 94032 | CAMK2N2 | calcium/calmodulin dependen... | NM_033259.3 | 99.5% | 98.7% | 237_238insG |
| 2 | mouse | 73047 | Camk2n2 | calcium/calmodulin-dependen... | NM_028420.2 | 94.9% | 97.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 309
- ORF length:
- 240
- Sequence:
-
1 tcttccattT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 CAGGCACCAT GTCCGAGATC CTGCCCTACA GCGAAGACAA GATGGGCCGC TTCGGCGCAG 121 ACCCCGAGGG CTCCGACCTC TCCTTCAGCT GCCGCCTGCA GGACACCAAC TCCTTCTTCG 181 CGGGCAACCA GGCCAAGCGA CCCCCCAAGC TGGGCCAGAT CGGCCGAGCC AAGCGAGTGG 241 TGATCGAGGA TGACCGGATA GACGACGTGC TGAAGGGGAT GGGGGAGAAG CCGCCGTCCG 301 GAGTGGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 361 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 421 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATCGGCGAG AATGGCTCGT AACCGACGCG 481 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt