Construct: ORF TRCN0000487975
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021937.1_s317c1
- DNA Barcode:
- AATAATATCATATCAACGCCATTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TFE3 (7030)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487975
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7030 | TFE3 | transcription factor bindin... | NM_006521.6 | 99.9% | 100% | 1323A>G |
| 2 | human | 7030 | TFE3 | transcription factor bindin... | NM_001282142.2 | 81.6% | 81.7% | 0_1ins315;1008A>G |
| 3 | human | 7030 | TFE3 | transcription factor bindin... | XM_024452432.1 | 77.1% | 69.4% | 1137_1182del;1369A>G;1413_1414ins358 |
| 4 | mouse | 209446 | Tfe3 | transcription factor E3 | NM_172472.3 | 89.6% | 95.8% | (many diffs) |
| 5 | mouse | 209446 | Tfe3 | transcription factor E3 | NM_001105196.1 | 84% | 89.9% | (many diffs) |
| 6 | mouse | 209446 | Tfe3 | transcription factor E3 | NM_001105197.1 | 72.9% | 78.4% | (many diffs) |
| 7 | mouse | 209446 | Tfe3 | transcription factor E3 | NM_001271489.1 | 72.9% | 78.4% | (many diffs) |
| 8 | mouse | 209446 | Tfe3 | transcription factor E3 | NM_001271490.1 | 72.9% | 78.4% | (many diffs) |
| 9 | mouse | 209446 | Tfe3 | transcription factor E3 | XM_006527578.2 | 72.9% | 78.4% | (many diffs) |
| 10 | mouse | 209446 | Tfe3 | transcription factor E3 | XM_017318453.1 | 72.9% | 78.4% | (many diffs) |
| 11 | mouse | 209446 | Tfe3 | transcription factor E3 | NM_001271491.1 | 67.2% | 72.5% | (many diffs) |
| 12 | mouse | 209446 | Tfe3 | transcription factor E3 | XM_017318454.1 | 67.2% | 72.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1794
- ORF length:
- 1725
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtctcatgcg gccgaaccag ctcgggatgg cgtagaggcc agcgcggagg 121 gccctcgagc cgtgttcgtg ctgttggagg agcgcaggcc ggccgactcg gctcagctgc 181 tcagcctgaa ctctttgctt ccggaatccg ggattgttgc tgacatagaa ttagaaaacg 241 tccttgatcc tgacagcttc tacgagctca aaagccaacc cttacccctt cgctcaagcc 301 tcccaatatc actgcaggcc acaccagcca ccccagctac actctctgca tcgtcttctg 361 cagggggctc caggacccct gccatgtcgt catcttcttc atcgagggtc ttgctgcggc 421 agcagctaat gcgggcccag gcgcaggagc aggagaggcg tgagcgtcgg gaacaggccg 481 ccgcggctcc cttccccagt cctgcacctg cctctcctgc catctctgtg gttggcgtct 541 ctgctggggg ccacacattg agccgtccac cccctgctca ggtgcccagg gaggtgctca 601 aggtgcagac ccatctggag aacccaacgc gctaccacct gcagcaggcg cgccggcagc 661 aggtgaaaca gtacctgtcc accacactcg ggcccaagct ggcttcccag gccctcaccc 721 caccgccggg gcccgcaagt gcccagccac tgcctgcccc tgaggctgcc cacactaccg 781 gccccacagg cagtgcgccc aacagcccca tggcgctgct caccatcggg tccagctcag 841 agaaggagat tgatgatgtc attgatgaga tcatcagcct ggagtccagt tacaatgatg 901 aaatgctcag ctatctgccc ggaggcacca caggactgca gctccccagc acgctgcctg 961 tgtcagggaa tctgcttgat gtgtacagta gtcaaggcgt ggccacacca gccatcactg 1021 tcagcaactc ctgcccagct gagctgccca acatcaaacg ggagatctct gagaccgagg 1081 caaaggccct tttgaaggaa cggcagaaga aagacaatca caacctaatt gagcgtcgca 1141 ggcgattcaa cattaacgac aggatcaagg aactgggcac tctcatccct aagtccagtg 1201 acccggagat gcgctggaac aagggcacca tcctgaaggc ctctgtggat tatatccgca 1261 agctgcagaa ggagcagcag cgctccaaag acctggagag ccggcagcga tccctggagc 1321 aggccaaccg cagcctgcag ctccgaattc aggaactaga actgcaggcc cagatccatg 1381 gcctgccagt gcctcccact ccagggctgc tttccttggc cacgacttcg gcttctgaca 1441 gcctcaagcc agagcagctg gacattgagg aggagggcag gccaggcgca gcaacgttcc 1501 atgtaggggg gggacctgcc cagaatgctc cccatcagca gccccctgca ccgccctcag 1561 atgcccTTCT GGACCTGCAC TTTCCCAGCG ACCACCTGGG GGACCTGGGA GACCCCTTCC 1621 ACCTGGGGCT GGAGGACATT CTGATGGAGG AGGAGGAGGG GGTGGTGGGA GGACTGTCGG 1681 GGGGTGCCCT GTCCCCACTG CGGGCTGCCT CCGATCCCCT GCTCTCTTCA GTGTCCCCTG 1741 CTGTCTCCAA GGCCAGCAGC CGCCGCAGCA GCTTCAGCAT GGAAGAGGAG TCCTAGAACC 1801 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1861 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1921 ATATATCTTG TGGAAAGGAC GAAATAATAT CATATCAACG CCATTCACGC GTTAAGTCga 1981 caatcaacct ctggattaca aaatttgtga aagatt