Construct: ORF TRCN0000487976

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021637.1_s317c1
DNA Barcode:
ACCACTAAGGGACAGCAAACCTTC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
HNF4G (3174)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487976
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3174 HNF4G hepatocyte nuclear factor 4... NM_001330561.2 99.9% 99.7% 570G>A
2 human 3174 HNF4G hepatocyte nuclear factor 4... XM_017013373.1 99.9% 99.7% 570G>A
3 human 3174 HNF4G hepatocyte nuclear factor 4... XM_017013374.1 99.9% 99.7% 570G>A
4 human 3174 HNF4G hepatocyte nuclear factor 4... XM_017013375.1 99.9% 99.7% 570G>A
5 human 3174 HNF4G hepatocyte nuclear factor 4... XM_017013376.2 99.9% 99.7% 570G>A
6 human 3174 HNF4G hepatocyte nuclear factor 4... NM_004133.5 89.5% 89.4% 1_141del;711G>A
7 mouse 30942 Hnf4g hepatocyte nuclear factor 4... NM_013920.2 84.7% 93.3% (many diffs)
8 mouse 30942 Hnf4g hepatocyte nuclear factor 4... XM_006530072.3 76.7% 84.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1296
ORF length:
1224
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgaatacc acagacaacg gtgtcaactg tctgtgtgct atctgtgggg
121acagagcaac aggaaaacac tatggggcat ccagctgtga tgggtgcaag ggtttcttca
181gacgcagcat tcgtaagagt cacgtttatt cttgcaggtt cagtcggcaa tgtgttgttg
241acaaggacaa aaggaatcaa tgtagatatt gtcgattaag aaagtgtttt agagcgggaa
301tgaaaaaaga agctgtacaa aatgaacgtg acagaataag caccagaaga agcacatttg
361atggcagcaa catcccctcc attaacacac tggcacaagc tgaagttcgg tctcgccaga
421tctcagtctc aagccctggg tcaagcactg acataaacgt taagaaaatt gcaagtattg
481gtgatgtctg tgaatctatg aaacagcagc tcttagtctt ggtggaatgg gctaaatata
541ttcctgcctt ctgtgaatta ccattggatg atcaggtggc actgttgaga gctcacgcag
601gggagcactt actgcttgga gctacaaaga gatccatgat atataaagat attttgcttt
661tgggaaacaa ctatgttatt caccgcaaca gctgtgaagt tgagattagc cgtgtggcca
721atcgtgttct agatgagctg gttagaccat ttcaagaaat ccagattgat gacaatgagt
781atgcttgttt aaaggcaatt gtattttttg atccagatgc aaaagggcta agcgatccag
841taaaaattaa gaacatgagg ttccaagtgc agatcggttt ggaggactac atcaatgatc
901ggcagtatga ctcccggggg aggtttggag agttgcttct gctcctgccc acactgcaga
961gcatcacgtg gcaaatgatt GAGCAAATAC AGTTTGTTAA ACTTTTTGGG ATGGTTAAAA
1021TTGACAATCT ACTTCAGGAA ATGCTATTAG GTGGGGCTTC CAATGATGGC AGTCATCTCC
1081ATCATCCAAT GCATCCACAT TTGTCTCAAG ACCCATTAAC TGGACAAACT ATACTTTTAG
1141GTCCCATGTC AACACTGGTT CATGCAGACC AGATCTCAAC TCCTGAAACC CCACTCCCTT
1201CCCCACCACA AGGCTCTGGG CAAGAACAGT ACAAAATAGC TGCAAACCAA GCATCAGTCA
1261TTTCACACCA GCATCTCTCC AAACAAAAGC AATTGTGAGA CCCAGCTTTC TTGTACAAAG
1321TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1381GAACTAGTCC GTAACCTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG
1441GACGAACCAC TAAGGGACAG CAAACCTTCA CGCGTTAAGT Cgacaatcaa cctctggatt
1501acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)