Construct: ORF TRCN0000487978
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021237.1_s317c1
- DNA Barcode:
- ACGATCAAACATCGATTTGACGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SBNO1 (55206)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487978
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55206 | SBNO1 | strawberry notch homolog 1 | NM_018183.5 | 20.1% | 20.1% | 1_3333del;3345T>C |
| 2 | human | 55206 | SBNO1 | strawberry notch homolog 1 | NM_001167856.3 | 20.1% | 20.1% | 1_3336del;3348T>C |
| 3 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530322.3 | 18.2% | 20.5% | (many diffs) |
| 4 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530323.3 | 18.2% | 20.5% | (many diffs) |
| 5 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530320.3 | 18.2% | 20.5% | (many diffs) |
| 6 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530321.3 | 18.2% | 20.5% | (many diffs) |
| 7 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530319.3 | 17.7% | 20% | (many diffs) |
| 8 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | NM_001081203.1 | 17.7% | 19.9% | (many diffs) |
| 9 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530317.3 | 17.7% | 19.9% | (many diffs) |
| 10 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530318.3 | 17.7% | 19.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 59
- ORF end:
- 902
- ORF length:
- 843
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaccat 61 ggaggtgcac cagcagaatg cgttatttca gtattttgcg gacacactta ctgcagttgt 121 tcaaaatgcc aaaaaaaatg gaagatatga tatgggaatc ttagatcttg gttctggaga 181 tgaaaaagtg cggaaaagtg atgttaaaaa gtttctgact ccaggatatt caacctctgg 241 ccacgtagaa ttatacacaa ttagtgtaga gaggggaatg tcatgggagg aagctaccaa 301 gatttgggct gagctgacag gaccagacga tggcttttac ttgtcattgc aaataaggaa 361 caacaagaaa actgccatct tagttaaaga agtgaatcct aaaaagaaac ttttcttagt 421 ttatcgacca aatactggga agcagctcaa attagaaatt tatgctgatc taaaaaagaa 481 atataagaag gtcgtctcag atgatgccct gatgcactgg ttagatcagt ataattcatc 541 tgcagatact tgtactcatg cttattggcg cggcaattGC AAAAAAGCAA GCTTGGGGCT 601 AGTTTGTGAA ATAGGTCTTC GTTGCCGTAC ATATTATGTA TTATGTGGTT CAGTGCTGAG 661 TGTCTGGACA AAAGTTGAGG GTGTTCTAGC ATCTGTCAGT GGCACAAACG TGAAGATGCA 721 GATCGTGCGG CTAAGAACGG AAGATGGGCA ACGGATTGTA GGTTTGATCA TTCCGGCAAA 781 TTGTGTGTCT CCTCTTGTAA ATCTCCTATC AACTTCAGAC CAGTCTCAAC AGCTTGCGGT 841 CCAACAGAAA CAGCTATGGC AACAGCATCA CCCTCAGAGC ATCACCAACT TGAGCAACGC 901 ATGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT 961 CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC 1021 TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACGATCAAAC ATCGATTTGA CGCCACGCGT 1081 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt