Construct: ORF TRCN0000487980
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020938.1_s317c1
- DNA Barcode:
- GTCACACCAAAATTATTAAGGAAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SMO (6608)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487980
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6608 | SMO | smoothened, frizzled class ... | NM_005631.5 | 99.9% | 100% | 1164G>C |
| 2 | human | 6608 | SMO | smoothened, frizzled class ... | XM_024446891.1 | 83.4% | 83.4% | 0_1ins390;774G>C |
| 3 | mouse | 319757 | Smo | smoothened, frizzled class ... | NM_176996.4 | 89.4% | 92.8% | (many diffs) |
| 4 | mouse | 319757 | Smo | smoothened, frizzled class ... | XM_006505106.4 | 75% | 79% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2433
- ORF length:
- 2361
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccgct gcccgcccag cgcgggggcc ggagctcccg ctcctggggc 121 tgctgctgct gctgctgctg ggggacccgg gccggggggc ggcctcgagc gggaacgcga 181 ccgggcctgg gcctcggagc gcgggcggga gcgcgaggag gagcgcggcg gtgactggcc 241 ctccgccgcc gctgagccac tgcggccggg ctgccccctg cgagccgctg cgctacaacg 301 tgtgcctggg ctcggtgctg ccctacgggg ccacctccac actgctggcc ggagactcgg 361 actcccagga ggaagcgcac ggcaagctcg tgctctggtc gggcctccgg aatgcccccc 421 gctgctgggc agtgatccag cccctgctgt gtgccgtata catgcccaag tgtgagaatg 481 accgggtgga gctgcccagc cgtaccctct gccaggccac ccgaggcccc tgtgccatcg 541 tggagaggga gcggggctgg cctgacttcc tgcgctgcac tcctgaccgc ttccctgaag 601 gctgcacgaa tgaggtgcag aacatcaagt tcaacagttc aggccagtgc gaagtgccct 661 tggttcggac agacaacccc aagagctggt acgaggacgt ggagggctgc ggcatccagt 721 gccagaaccc gctcttcaca gaggctgagc accaggacat gcacagctac atcgcggcct 781 tcggggccgt cacgggcctc tgcacgctct tcaccctggc cacattcgtg gctgactggc 841 ggaactcgaa tcgctaccct gctgttattc tcttctacgt caatgcgtgc ttctttgtgg 901 gcagcattgg ctggctggcc cagttcatgg atggtgcccg ccgagagatc gtctgccgtg 961 cagatggcac catgaggctt ggggagccca cctccaatga gactctgtcc tgcgtcatca 1021 tctttgtcat cgtgtactac gccctgatgg ctggtgtggt ttggtttgtg gtcctcacct 1081 atgcctggca cacttccttc aaagccctgg gcaccaccta ccagcctctc tcgggcaaga 1141 cctcctactt ccacctgctc acctggtcac tcccctttgt cctcactgtg gcaatccttg 1201 ctgtggcgca ggtggatggg gactctgtga gtggcatttg ttttgtgggc tacaagaact 1261 accgataccg tgcgggcttc gtgctggccc caatcggcct ggtgctcatc gtgggaggct 1321 acttcctcat ccgaggagtc atgactctgt tctccatcaa gagcaaccac cccgggctgc 1381 tgagtgagaa ggctgccagc aagatcaacg agaccatgct gcgcctgggc atttttggct 1441 tcctggcctt tggctttgtg ctcattacct tcagctgcca cttctacgac ttcttcaacc 1501 aggctgagtg ggagcgcagc ttccgggact atgtgctatg tcaggccaat gtgaccatcg 1561 ggctgcccac caagcagccc atccctgact gtgagatcaa gaatcgcccg agccttctgg 1621 tggagaagat caacctgttt gccatgtttg gaactggcat cgccatgagc acctgggtct 1681 ggaccaaggc cacgctgctc atctggaggc gtacctggtg caggttgact gggcagagtg 1741 acgatgagcc aaagcggatc aagaagagca agatgattgc caaggccttc tctaagcggc 1801 acgagctcct gcagaaccca ggccaggagc tgtccttcag catgcacact gtgtcccacg 1861 acgggcccgt ggcgggcttg gcctttgacc tcaatgagcc ctcagctgat gtctcctctg 1921 cctgggccca gcatgtcacc aagatggtgg ctcggagagg agccatactg ccccaggata 1981 tttctgtcac ccctgtggca actccagtgc ccccagagga acaagccaac ctgtggctgg 2041 ttgaggcaga gatctcccca gagctgcaga agcgcctggg ccggaagaag aagaggagga 2101 agaggaagaa ggaggtgtgc ccgctggcgc cgccccctga gcttcacccc cctgcccctg 2161 cccccagtac cattcctcga ctgccTCAGC TGCCCCGGCA GAAATGCCTG GTGGCTGCAG 2221 GTGCCTGGGG AGCTGGGGAC TCTTGCCGAC AGGGAGCGTG GACCCTGGTC TCCAACCCAT 2281 TCTGCCCAGA GCCCAGTCCC CCTCAGGATC CATTTCTGCC CAGTGCACCG GCCCCCGTGG 2341 CATGGGCTCA TGGCCGCCGA CAGGGCCTGG GGCCTATTCA CTCCCGCACC AACCTGATGG 2401 ACACAGAACT CATGGATGCA GACTCGGACT TCTAGGACCC AGCTTTCTTG TACAAAGTGG 2461 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2521 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2581 AGTCACACCA AAATTATTAA GGAAAACGCG TTAAGTCgac aatcaacctc tggattacaa 2641 aatttgtgaa agatt