Construct: ORF TRCN0000487981
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020901.1_s317c1
- DNA Barcode:
- GCCCATGCGTTCTCACGCCCGATT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- OPN3 (23596)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487981
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23596 | OPN3 | opsin 3 | NM_014322.3 | 100% | 100% | |
| 2 | mouse | 13603 | Opn3 | opsin 3 | NM_010098.3 | 85% | 87% | (many diffs) |
| 3 | mouse | 13603 | Opn3 | opsin 3 | XM_006496642.2 | 59.7% | 61.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1278
- ORF length:
- 1206
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtactcg gggaaccgca gcggcggcca cggctactgg gacggcggcg 121 gggccgcggg cgctgagggg ccggcgccgg cggggacact gagccccgcg cccctcttca 181 gccccggcac ctacgagcgc ctggcgctgc tgctgggctc cattgggctg ctgggcgtcg 241 gcaacaacct gctggtgctc gtcctctact acaagttcca gcggctccgc actcccactc 301 acctcctcct ggtcaacatc agcctcagcg acctgctggt gtccctcttc ggggtcacct 361 ttaccttcgt gtcctgcctg aggaacggct gggtgtggga caccgtgggc tgcgtgtggg 421 acgggtttag cggcagcctc ttcgggattg tttccattgc caccctaacc gtgctggcct 481 atgaacgtta cattcgcgtg gtccatgcca gagtgatcaa tttttcctgg gcctggaggg 541 ccattaccta catctggctc tactcactgg cgtgggcagg agcacctctc ctgggatgga 601 acaggtacat cctggacgta cacggactag gctgcactgt ggactggaaa tccaaggatg 661 ccaacgattc ctcctttgtg cttttcttat ttcttggctg cctggtggtg cccctgggtg 721 tcatagccca ttgctatggc catattctat attccattcg aatgcttcgt tgtgtggaag 781 atcttcagac aattcaagtg atcaagattt taaaatatga aaagaaactg gccaaaatgt 841 gctttttaat gatattcacc ttcctggtct gttggatgcc ttatatcgtg atctgcttct 901 tggtggttaa tggtcatggt cacctggtca ctccaacaat atctattgtt tcgtacctct 961 tTGCTAAATC GAACACTGTA TACAATCCAG TGATTTATGT CTTCATGATC AGAAAGTTTC 1021 GAAGATCCCT TTTGCAGCTT CTGTGCCTCC GACTGCTGAG GTGCCAGAGG CCTGCTAAAG 1081 ACCTACCAGC AGCTGGAAGT GAAATGCAGA TCAGACCCAT TGTGATGTCA CAGAAAGATG 1141 GGGACAGGCC AAAGAAAAAA GTGACTTTCA ACTCTTCTTC CATCATTTTT ATCATCACCA 1201 GTGATGAATC ACTGTCAGTT GACGACAGCG ACAAAACCAA TGGGTCCAAA GTTGATGTAA 1261 TCCAAGTTCG TCCTTTGTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1321 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1381 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCCC ATGCGTTCTC 1441 ACGCCCGATT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt