Construct: ORF TRCN0000487988

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019925.1_s317c1
DNA Barcode:
TCACGCTACTACGAAGTGGAATCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PCSK9 (255738)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487988
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 255738 PCSK9 proprotein convertase subti... NM_174936.4 44.1% 40.5% (many diffs)
2 human 255738 PCSK9 proprotein convertase subti... NR_110451.1 28.6% (many diffs)
3 mouse 100102 Pcsk9 proprotein convertase subti... NM_153565.2 38.1% 36% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1017
ORF length:
948
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gtcgccttgg aaagacggag gcagcctggt ggaggtgtat ctcctagaca
121ccagcataca gagtgaccac cgggaaatcg agggcagggt catggtcacc gacttcgaga
181atgtgcccga ggaggacggg acccgcttcc acagacaggc cagcaagtgt gacagtcatg
241gcacccacct ggcaggagtg gtcagcggcc gggatgccgg cgtggccaag ggtgccagca
301tgcgcagcct gcgcgtgctc aactgccaag ggaagggcac ggttagcggc accctcatag
361gcctggagtt tattcggaaa agccagctgg tccagcctgt ggggccactg gtggtgctgc
421tgcccctggc gggtgggtac agccgcgtcc tcaacgccgc ctgccagcgc ctggcgaggg
481ctggggtcgt gctggtcacc gctgccggca acttccggga cgatgcctgc ctctactccc
541cagcctcagc tcccgaggtc atcacagttg gggccaccaa tgcccaggac cagccggtga
601ccctggggac tttggggacc aactttggcc gctgtgtgga cctctttgcc ccaggggagg
661acatcattgg tgcctccagc gactgcagca ccTGCTTTGT GTCACAGAGT GGGACATCAC
721AGGCTGCTGC CCACGTGGCT GGCATTGCAG CCATGATGCT GTCTGCCGAG CCGGAGCTCA
781CCCTGGCCGA GTTGAGGCAG AGACTGATCC ACTTCTCTGC CAAAGATGTC ATCAATGAGG
841CCTGGTTCCC TGAGGACCAG CGGGTACTGA CCCCCAACCT GGTGGCCGCC CTGCCCCCCA
901GCACCCATGG GGCAGGCCCT TTTTGCAGGT TGGCAGCTGT TTTGCAGGAC TGTGTGGTCA
961GCACACTCGG GGCCTACACG GATGGCCACA GCCATCGCCC GCTGCGCCCC AGAGACCCAG
1021CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1081ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1141TATCTTGTGG AAAGGACGAT CACGCTACTA CGAAGTGGAA TCAACGCGTT AAGTCgacaa
1201tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)