Construct: ORF TRCN0000487994
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021898.1_s317c1
- DNA Barcode:
- CTGAAGCCCTCGAGTCATAATCTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HAS1 (3036)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487994
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3036 | HAS1 | hyaluronan synthase 1 | NM_001297436.2 | 99.8% | 99.6% | 37T>C;606C>T;685G>A |
| 2 | human | 3036 | HAS1 | hyaluronan synthase 1 | NM_001523.4 | 99.6% | 99.1% | (many diffs) |
| 3 | human | 3036 | HAS1 | hyaluronan synthase 1 | XM_011526884.2 | 56.4% | 51.1% | (many diffs) |
| 4 | mouse | 15116 | Has1 | hyaluronan synthase 1 | NM_008215.2 | 84.1% | 95.3% | (many diffs) |
| 5 | mouse | 15116 | Has1 | hyaluronan synthase 1 | XM_011246309.2 | 83.2% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1803
- ORF length:
- 1731
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagacag gacgcgccca agcccactcc tgcagcccgc cgctgctccg 121 gcctggcccg gagggtgctg accatcgcct tcgccctgct catcctgggc ctcatgacct 181 gggcctacgc cgccggggtg ccgctggcct ccgatcgcta cggcctcctg gccttcggcc 241 tctacggggc cttcctttca gcgcacctgg tggcgcagag cctcttcgcg tacctggagc 301 accggcgggt ggcggcggcg gcgcgggggc cgctggatgc agccaccgcg cgcagtgtgg 361 cgctgaccat ctccgcctac caggaggacc ccgcgtacct gcgccagtgc ctggcgtccg 421 cccgcgccct gctgtacccg cgcgcgcggc tgcgcgtcct catggtggtg gatggcaacc 481 gcgccgagga cctctacatg gtcgacatgt tccgcgaggt cttcgctgac gaggaccccg 541 ccacgtacgt gtgggacggc aactaccacc agccctggga acccgcggcg gcgggcgcgg 601 tgggcgccgg agcctatcgg gaggtggagg cggaggatcc tgggcggctg gcagtggagg 661 cgctggtgag gactcgtagg tgcgtgtgcg tggcgcagcg ctggggcggc aagcgcgagg 721 tcatgtacac agccttcaag gcgctcggag attcgatgga ctacgtgcag gtctgtgact 781 cggacacaag gttggacccc atggcactgc tggagctcgt gcgggtactg gacgaggacc 841 cccgggtagg ggctgttggt ggggacgtgc ggatccttaa ccctctggac tcctgggtca 901 gcttcctaag cagcctgcga tactgggtag ccttcaatgt ggagcgggct tgtcagagct 961 acttccactg tgtatcctgc atcagcggtc ctctaggcct atataggaat aacctcttgc 1021 agcagtttct tgaggcctgg tacaaccaga agttcctggg tacccactgt acttttgggg 1081 atgaccggca cctcaccaac cgcatgctca gcatgggtta tgctaccaag tacacctcca 1141 ggtcccgctg ctactcagag acgccctcgt ccttcctgcg gtggctgagc cagcagacac 1201 gctggtccaa gtcgtacttc cgtgagtggc tgtacaacgc gctctggtgg caccggcacc 1261 atgcgtggat gacctacgag gcggtggtct ccggcctgtt ccccttcttc gtggcggcca 1321 ctgtgctgcg tctgttctac gcgggccgcc cttgggcgct gctgtgggtg ctgctgtgcg 1381 tgcagggcgt ggcactggcc aaggcggcct tcgcggcctg gctgcggggc tgcctgcgca 1441 tggtgcttct gtcgctctac gcgcccctct acatgtgtgg ccTCCTGCCT GCCAAGTTCC 1501 TGGCGCTAGT CACCATGAAC CAGAGTGGCT GGGGCACCTC GGGCCGGCGG AAGCTGGCCG 1561 CTAACTACGT CCCTCTGCTG CCCCTGGCGC TCTGGGCGCT GCTGCTGCTT GGGGGCCTGG 1621 TCCGCAGCGT AGCACACGAG GCCAGGGCCG ACTGGAGCGG CCCTTCCCGC GCAGCCGAGG 1681 CCTACCACTT GGCCGCGGGG GCCGGCGCCT ACGTGGGCTA CTGGGTGGCC ATGTTGACGC 1741 TGTACTGGGT GGGCGTGCGG AGGCTTTGCC GGCGGCGGAC CGGGGGCTAC CGCGTCCAGG 1801 TGTGAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1861 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1921 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTGAAGCCC TCGAGTCATA ATCTAACGCG 1981 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt