Construct: ORF TRCN0000487996
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021331.1_s317c1
- DNA Barcode:
- GCGGGCGTCCTAGTAGTAAACTCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NUP37 (79023)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487996
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79023 | NUP37 | nucleoporin 37 | NM_024057.4 | 69% | 69% | 1_303del |
| 2 | human | 79023 | NUP37 | nucleoporin 37 | XR_944712.2 | 40.2% | 1_450del;688_845del;1284_1678del | |
| 3 | mouse | 69736 | Nup37 | nucleoporin 37 | NM_027191.2 | 60.1% | 62.5% | (many diffs) |
| 4 | mouse | 69736 | Nup37 | nucleoporin 37 | NM_028334.4 | 60.1% | 62.5% | (many diffs) |
| 5 | mouse | 69736 | Nup37 | nucleoporin 37 | XM_006514057.2 | 44.2% | 43.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 747
- ORF length:
- 675
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaaaatt agattattta cttcagatct tcaggataaa aatgaatata 121 aggttttaga gggccatacc gatttcatta atggtttggt gtttgatccc aaagaaggcc 181 aagaaattgc aagtgtgagt gacgatcaca cctgcaggat ttggaacttg gaaggagtgc 241 aaacagctca ttttgttctt cattctcctg gcatgagtgt gtgctggcat cctgaggaga 301 cttttaagct aatggttgca gagaagaatg gaacaatccg gttttatgat cttttggccc 361 aacaggctat tttatctctt gaatcagaac aagtgccatt aatgtcagca cactggtgct 421 taaaaaacac cttcaaagtt gGAGCCGTTG CAGGAAATGA TTGGTTAATT TGGGATATTA 481 CTCGGTCCAG TTATCCTCAA AATAAGAGAC CTGTTCACAT GGATCGAGCC TGCTTATTCA 541 GGTGGTCCAC AATTAGTGAA AATCTGTTTG CAACCACTGG TTATCCTGGC AAAATGGCAA 601 GCCAGTTTCA AATTCATCAT TTAGGACACC CTCAGCCCAT CCTCATGGGT TCTGTAGCCG 661 TTGGATCTGG ACTGTCCTGG CATCGAACTC TCCCTCTGTG TGTAATTGGA GGAGACCACA 721 AGCTGTTGTT TTGGGTGACT GAAGTATAGA ACCCAGCTTT CTTGTACAAA GTGGTTGATA 781 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 841 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATACT TGTGGAAAGG ACGAGCGGGC 901 GTCCTAGTAG TAAACTCTAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 961 gaaagatt