Construct: ORF TRCN0000487999
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020846.1_s317c1
- DNA Barcode:
- CTTTTAAACGAATCGTATGACAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADORA2A (135)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487999
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 135 | ADORA2A | adenosine A2a receptor | NM_000675.6 | 99.9% | 100% | 1083T>C |
| 2 | human | 135 | ADORA2A | adenosine A2a receptor | NM_001278497.1 | 99.9% | 100% | 1083T>C |
| 3 | human | 135 | ADORA2A | adenosine A2a receptor | NM_001278498.1 | 99.9% | 100% | 1083T>C |
| 4 | human | 135 | ADORA2A | adenosine A2a receptor | NM_001278499.2 | 99.9% | 100% | 1083T>C |
| 5 | human | 135 | ADORA2A | adenosine A2a receptor | NM_001278500.1 | 99.9% | 100% | 1083T>C |
| 6 | human | 135 | ADORA2A | adenosine A2a receptor | NR_103543.2 | 47.5% | (many diffs) | |
| 7 | human | 135 | ADORA2A | adenosine A2a receptor | NR_103544.2 | 47.4% | (many diffs) | |
| 8 | human | 101730217 | SPECC1L-ADORA2A | SPECC1L-ADORA2A readthrough... | NR_103546.1 | 19.6% | 1_4179del;5262T>C;5416_6289del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1308
- ORF length:
- 1236
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcccatc atgggctcct cggtgtacat cacggtggag ctggccattg 121 ctgtgctggc catcctgggc aatgtgctgg tgtgctgggc cgtgtggctc aacagcaacc 181 tgcagaacgt caccaactac tttgtggtgt cactggcggc ggccgacatc gcagtgggtg 241 tgctcgccat cccctttgcc atcaccatca gcaccgggtt ctgcgctgcc tgccacggct 301 gcctcttcat tgcctgcttc gtcctggtcc tcacgcagag ctccatcttc agtctcctgg 361 ccatcgccat tgaccgctac attgccatcc gcatcccgct ccggtacaat ggcttggtga 421 ccggcacgag ggctaagggc atcattgcca tctgctgggt gctgtcgttt gccatcggcc 481 tgactcccat gctaggttgg aacaactgcg gtcagccaaa ggagggcaag aaccactccc 541 agggctgcgg ggagggccaa gtggcctgtc tctttgagga tgtggtcccc atgaactaca 601 tggtgtactt caacttcttt gcctgtgtgc tggtgcccct gctgctcatg ctgggtgtct 661 atttgcggat cttcctggcg gcgcgacgac agctgaagca gatggagagc cagcctctgc 721 cgggggagcg ggcacggtcc acactgcaga aggaggtcca tgctgccaag tcactggcca 781 tcattgtggg gctctttgcc ctctgctggc tgcccctaca catcatcaac tgcttcactt 841 tcttctgccc cgactgcagc cacgcccctc tctggctcat gtacctggcc atcgtcctct 901 cccacaccaa ttcggttgtg aatcccttca tctacgccta ccgtatccgc gagttccgcc 961 agaccttccg caagatcatt cgcagccacg tcctgaggca gcaagaaCCT TTCAAGGCAG 1021 CTGGCACCAG TGCCCGGGTC TTGGCAGCTC ATGGCAGTGA CGGAGAGCAG GTCAGCCTCC 1081 GTCTCAACGG CCACCCGCCA GGAGTGTGGG CCAACGGCAG TGCTCCCCAC CCTGAGCGGA 1141 GGCCCAATGG CTACGCCCTG GGGCTGGTGA GTGGAGGGAG TGCCCAAGAG TCCCAGGGGA 1201 ACACGGGCCT CCCAGACGTG GAGCTCCTTA GCCATGAGCT CAAGGGAGTG TGCCCAGAGC 1261 CCCCTGGCCT AGATGACCCC CTGGCCCAGG ATGGAGCAGG AGTGTCCTAG GACCCAGCTT 1321 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1381 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1441 CTTGTGGAAA GGACGACTTT TAAACGAATC GTATGACAGA ACGCGTTAAG TCgacaatca 1501 acctctggat tacaaaattt gtgaaagatt