Construct: ORF TRCN0000488014
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019434.1_s317c1
- DNA Barcode:
- ACAACGACTACCCACGCTATACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MOXD1 (26002)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488014
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26002 | MOXD1 | monooxygenase DBH like 1 | XM_017010714.2 | 91.3% | 89.7% | (many diffs) |
| 2 | human | 26002 | MOXD1 | monooxygenase DBH like 1 | NM_015529.4 | 87% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1704
- ORF length:
- 1638
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat 61 tgaccatgaa ttcttttctc tctgtattta ggtatcctga tttttccttt ccatattttc 121 cacaggatta ttttacaaat gcaaatagag agttgaaaaa agatgcccag caagattacc 181 atctagaata tgccatggaa aatagcacac acacaataat tgaatttacc agagagctgc 241 atacatgtga cataaatgac aagagtataa cggatagcac tgtgagagtg atctgggcct 301 accaccatga agatgcagga gaagctggtc ccaagtacca tgactccaat aggggcacca 361 agagtttgcg gttattgaat cctgagaaaa ctagtgtgct atctacagcc ttaccatact 421 ttgatctggt aaatcaggac gtccccatcc caaacaaaga tacaacatat tggtgccaaa 481 tgtttaagat tcctgtgttc caagaaaagc atcatgtaat aaaggttgag ccagtgatac 541 agagaggcca tgagagtctg gtgcaccaca tcctgctcta tcagtgcagc aacaacttta 601 acgacagcgt tctggagtcc ggccacgagt gctatcaccc caacatgccc gatgcattcc 661 tcacctgtga aactgtgatt tttgcctggg ctattggtgg agagggcttt tcttatccac 721 ctcatgttgg attatccctt ggcactccat tagatccgca ttatgtgctc ctagaagtcc 781 attatgataa tcccacttat gaggaaggct taatagataa ttctggactg aggttatttt 841 acacaatgga tataaggaaa tatgatgctg gggtgattga ggctggcctc tgggtgagcc 901 tcttccatac catccctcca gggatgcctg agttccagtc tgagggtcac tgcactttgg 961 agtgcctgga agaggctctg gaagccgaaa agccaagtgg aattcatgtg tttgctgttc 1021 ttctccatgc tcacctggct ggcagaggca tcaggctgcg tcattttcga aaagggaagg 1081 aaatgaaatt acttgcctat gatgatgatt ttgacttcaa tttccaggag tttcagtatc 1141 taaaggaaga acaaacaatc ttaccaggag ataacctaat tactgagtgt cgctacaaca 1201 cgaaagatag agctgagatg acttggggag gactaagcac caggagtgaa atgtgtctct 1261 cataccttct ttattaccca agaattaatc ttactcgatg tgcaagtatt ccagacatta 1321 tggaacaact tcagttcatt ggggttaagg agatctacag accagtcacg acctggcctt 1381 tcattatcaa aagtcccaag caatataaaa aCCTTTCTTT CATGGATGCT ATGAATAAGT 1441 TTAAATGGAC TAAAAAGGAA GGTCTCTCCT TCAACAAGCT GGTCCTCAGC CTGCCAGTGA 1501 ATGTGAGATG TTCCAAGACA GACAATGCTG AGTGGTCGAT TCAAGGAATG ACAGCATTAC 1561 CTCCAGATAT AGAAAGACCC TATAAAGCAG AACCTTTGGT GTGTGGCACG TCTTCTTCCT 1621 CTTCCCTGCA CAGAGATTTC TCCATCAACT TGCTTGTTTG CCTTCTGCTA CTCAGCTGCA 1681 CGCTGAGCAC CAAGAGCTTG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1741 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1801 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACAA CGACTACCCA 1861 CGCTATACGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt