Construct: ORF TRCN0000488019
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020753.1_s317c1
- DNA Barcode:
- GACCGTGCGAGCACTCACGAGTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- QRFPR (84109)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488019
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84109 | QRFPR | pyroglutamylated RFamide pe... | NM_198179.3 | 99.9% | 100% | 816C>T |
| 2 | human | 84109 | QRFPR | pyroglutamylated RFamide pe... | XM_017008693.2 | 43.6% | 43.3% | (many diffs) |
| 3 | mouse | 229214 | Qrfpr | pyroglutamylated RFamide pe... | NM_198192.2 | 83.8% | 83.1% | (many diffs) |
| 4 | mouse | 243407 | C130060K24Rik | RIKEN cDNA C130060K24 gene | NM_175524.4 | 81.4% | 78.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1365
- ORF length:
- 1293
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcaggcg cttaacatta ccccggagca gttctctcgg ctgctgcggg 121 accacaacct gacgcgggag cagttcatcg ctctgtaccg gctgcgaccg ctcgtctaca 181 ccccagagct gccgggacgc gccaagctgg ccctcgtgct caccggcgtg ctcatcttcg 241 ccctggcgct ctttggcaat gctctggtgt tctacgtggt gacccgcagc aaggccatgc 301 gcaccgtcac caacatcttt atctgctcct tggcgctcag tgacctgctc atcaccttct 361 tctgcattcc cgtcaccatg ctccagaaca tttccgacaa ctggctgggg ggtgctttca 421 tttgcaagat ggtgccattt gtccagtcta ccgctgttgt gacagaaatc ctcactatga 481 cctgcattgc tgtggaaagg caccagggac ttgtgcatcc ttttaaaatg aagtggcaat 541 acaccaaccg aagggctttc acaatgctag gtgtggtctg gctggtggca gtcatcgtag 601 gatcacccat gtggcacgtg caacaacttg agatcaaata tgacttccta tatgaaaagg 661 aacacatctg ctgcttagaa gagtggacca gccctgtgca ccagaagatc tacaccacct 721 tcatccttgt catcctcttc ctcctgcctc ttatggtgat gcttattctg tacagtaaaa 781 ttggttatga actttggata aagaaaagag ttggggatgg ttcagtgctt cgaactattc 841 atggaaaaga aatgtccaaa atagccagga agaagaaacg agctgttatt atgatggtga 901 cagtggtggc tctctttgct gtgtgctggg caccattcca tgttgtccat atgatgattg 961 aatacagtaa ttttgaaaag gaatatgatg atgtcacaat caagatgatt tttgctatcg 1021 tgcaaattat tggattttcc aactccatct gtaatcccat tGTCTATGCA TTTATGAATG 1081 AAAACTTCAA AAAAAATGTT TTGTCTGCAG TTTGTTATTG CATAGTAAAT AAAACCTTCT 1141 CTCCAGCACA AAGGCATGGA AATTCAGGAA TTACAATGAT GCGGAAGAAA GCAAAGTTTT 1201 CCCTCAGAGA GAATCCAGTG GAGGAAACCA AAGGAGAAGC ATTCAGTGAT GGCAACATTG 1261 AAGTCAAATT GTGTGAACAG ACAGAGGAGA AGAAAAAGCT CAAACGACAT CTTGCTCTCT 1321 TTAGGTCTGA ACTGGCTGAG AATTCTCCTT TAGACAGTGG GCATTAGGAC CCAGCTTTCT 1381 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1441 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1501 GTGGAAAGGA CGAGACCGTG CGAGCACTCA CGAGTTAACG CGTTAAGTCg acaatcaacc 1561 tctggattac aaaatttgtg aaagatt