Construct: ORF TRCN0000488044
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019600.1_s317c1
- DNA Barcode:
- ACCAAGTACCCACTTCCCCATGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LMLN (89782)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488044
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89782 | LMLN | leishmanolysin like peptidase | NM_033029.3 | 99.9% | 99.8% | 1965_1966insG |
| 2 | human | 89782 | LMLN | leishmanolysin like peptidase | NM_001136049.2 | 94.6% | 94.5% | 1233_1343del;2076_2077insG |
| 3 | human | 89782 | LMLN | leishmanolysin like peptidase | NR_026787.2 | 26.2% | 1_5delACTCC;102_103ins121;1850_6905delinsG | |
| 4 | human | 89782 | LMLN | leishmanolysin like peptidase | NR_026786.2 | 25.8% | (many diffs) | |
| 5 | mouse | 239833 | Lmln | leishmanolysin-like (metall... | NM_172823.2 | 78.3% | 83.9% | (many diffs) |
| 6 | mouse | 239833 | Lmln | leishmanolysin-like (metall... | XM_006522123.1 | 49.1% | 54.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2034
- ORF length:
- 1968
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat 61 tgaccatggt aacgacgctc ggcccgaaga tggcggccga atggggcgga ggagtgggtt 121 actcgggctc aggcccgggc cggagccggt ggcgctggag cgggtctgtg tgggtccgaa 181 gcgttttact cctgttgggc gggctccggg ccagcgccac atctactccc gtctccttgg 241 gcagttcccc tccctgccgg caccacgtcc cctctgacac tgaggtcata aataaagttc 301 atcttaaggc aaatcatgtg gtcaagagag atgttgatga gcatttaaga atcaagactg 361 tctatgataa aagtgttgaa gagttgctcc ctgagaaaaa gaatcttgta aagaacaagc 421 ttttcccaca agcgatttct tatttagaga agacttttca ggtccgtcga cctgcgggca 481 ctatcttact tagcagacaa tgtgcaacaa accaatacct ccggaaggaa aacgatcctc 541 acaggtactg caccggggag tgtgccgcac acacaaagtg cggccccgtt attgttcctg 601 aggaacatct ccagcaatgc cgggtctacc gtgggggtaa gtggcctcat ggagcagtgg 661 gtgtgccaga ccaagaaggc atctcagatg cagactttgt tctttacgtt ggtgctctgg 721 ccaccgagag atgcagccat gaaaacatca tctcttatgc agcctattgt cagcaggaag 781 caaacatgga caggccaata gcaggatatg ctaacctgtg tccaaatatg atctctaccc 841 agcctcagga gtttgttggg atgctgtcca cagtgaaaca tgaggttatt catgccctgg 901 gtttctctgc tgggctgttt gcattctacc atgataaaga tggaaatcct ctcacttcaa 961 gatttgcaga tggcctccca ccttttaatt atagtctggg attatatcaa tggagtgata 1021 aagtagttcg aaaagtggag agattatggg atgttcgaga taataagata gttcgtcaca 1081 ctgtgtatct cctggtaacg cctcgtgttg ttgaggaagc acgaaaacat tttgattgtc 1141 cagttctaga gggaatggaa cttgaaaatc aaggtggtgt gggcactgag ctcaaccatt 1201 gggaaaaaag gttattagag aatgaagcga tgactggttc tcacactcag aatcgagtac 1261 tctctcgaat cactctggca ttaatggagg acactgggag acagatgctg agcccttact 1321 gtgacacgct cagaagtaac ccactgcagc taacttgcag acaggaccag agagcagttg 1381 ccgtgtgtaa tttgcagaag ttccctaagc ctttaccaca ggaataccag tactttgatg 1441 aactcagtgg aatacctgca gaagatttgc cttattatgg tggctccgtg gaaattgctg 1501 actactgccc tttcagtcag gaattcagtt ggcatttaag tggtgaatat cagcgcagct 1561 cagattgtag aatattggaa aatcaaccag aaatttttaa gaactatggc gctgaaaagt 1621 atggacctca ttccgtttgt ctaattcaga aatcagcatt cgttatggag aagtgtgaga 1681 ggaagctgag ttacccagac tggGGAAGCG GATGCTATCA GGTTTCTTGT TCTCCTCAAG 1741 GTCTGAAAGT TTGGGTCCAA GATACTTCAT ATTTGTGTAG TCGGGCTGGG CAGGTCCTCC 1801 CTGTCAGTAT CCAGATGAAT GGCTGGATTC ACGATGGAAA CCTGCTCTGC CCATCATGTT 1861 GGGACTTCTG TGAGCTCTGT CCTCCAGAAA CAGATCCTCC AGCCACTAAC CTGACCCGAG 1921 CTCTGCCACT TGATCTTTGT TCCTGTTCCT CGAGCCTGGT GGTCACCCTC TGGCTTCTGC 1981 TAGGCAATCT GTTTCCTCTG CTGGCTGGAT TTCTTCTGTG TATATGGCAC GACCCAGCTT 2041 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2101 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2161 CTTGTGGAAA GGACGAACCA AGTACCCACT TCCCCATGTA ACGCGTTAAG TCgacaatca 2221 acctctggat tacaaaattt gtgaaagatt