Construct: ORF TRCN0000488071
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021365.1_s317c1
- DNA Barcode:
- TACATCTGCGGGACACGCTTTAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADRB2 (154)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488071
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 154 | ADRB2 | adrenoceptor beta 2 | NM_000024.5 | 99.9% | 99.7% | 647T>C |
| 2 | mouse | 11555 | Adrb2 | adrenergic receptor, beta 2 | NM_007420.3 | 84.5% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1314
- ORF length:
- 1239
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgggg caacccggga acggcagcgc cttcttgctg gcacccaata 121 gaagccatgc gccggaccac gacgtcacgc agcaaaggga cgaggtgtgg gtggtgggca 181 tgggcatcgt catgtctctc atcgtcctgg ccatcgtgtt tggcaatgtg ctggtcatca 241 cagccattgc caagttcgag cgtctgcaga cggtcaccaa ctacttcatc acttcactgg 301 cctgtgctga tctggtcatg ggcctggcag tggtgccctt tggggccgcc catattctta 361 tgaaaatgtg gacttttggc aacttctggt gcgagttttg gacttccatt gatgtgctgt 421 gcgtcacggc cagcattgag accctgtgcg tgatcgcagt ggatcgctac tttgccatta 481 cttcaccttt caagtaccag agcctgctga ccaagaataa ggcccgggtg atcattctga 541 tggtgtggat tgtgtcaggc cttacctcct tcttgcccat tcagatgcac tggtaccggg 601 ccacccacca ggaagccatc aactgctatg ccaatgagac ctgctgtgac ttcttcacga 661 accaagccta tgccattgcc tcttccatcg tgtccttcta cgttcccctg gtgatcatgg 721 ccttcgtcta ctccagggtc tttcaggagg ccaaaaggca gctccagaag attgacaaat 781 ctgagggccg cttccatgtc cagaacctta gccaggtgga gcaggatggg cggacggggc 841 atggactccg cagatcttcc aagttctgct tgaaggagca caaagccctc aagacgttag 901 gcatcatcat gggcactttc accctctgct ggctgccctt cttcatcgtt aacattgtgc 961 atgtgatcca ggataacctc atccgtaagg aagtttacat cctcctaaat tGGATAGGCT 1021 ATGTCAATTC TGGTTTCAAT CCCCTTATCT ACTGCCGGAG CCCAGATTTC AGGATTGCCT 1081 TCCAGGAGCT TCTGTGCCTG CGCAGGTCTT CTTTGAAGGC CTATGGGAAT GGCTACTCCA 1141 GCAACGGCAA CACAGGGGAG CAGAGTGGAT ATCACGTGGA ACAGGAGAAA GAAAATAAAC 1201 TGCTGTGTGA AGACCTCCCA GGCACGGAAG ACTTTGTGGG CCATCAAGGT ACTGTGCCTA 1261 GCGATAACAT TGATTCACAA GGGAGGAATT GTAGTACAAA TGACTCACTG CTGTAGGACC 1321 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GATACATCTG CGGGACACGC TTTAGCACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt