Construct: ORF TRCN0000488075
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020905.1_s317c1
- DNA Barcode:
- AAGTAATATTACAGGTATATAGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PROKR2 (128674)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488075
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 128674 | PROKR2 | prokineticin receptor 2 | NM_144773.3 | 100% | 100% | |
| 2 | human | 128674 | PROKR2 | prokineticin receptor 2 | XM_017027646.1 | 100% | 100% | |
| 3 | human | 10887 | PROKR1 | prokineticin receptor 1 | NM_138964.3 | 82.5% | 84.9% | (many diffs) |
| 4 | mouse | 246313 | Prokr2 | prokineticin receptor 2 | NM_144944.3 | 85.6% | 89.5% | (many diffs) |
| 5 | mouse | 246313 | Prokr2 | prokineticin receptor 2 | XM_011239555.2 | 85.6% | 89.5% | (many diffs) |
| 6 | mouse | 246313 | Prokr2 | prokineticin receptor 2 | XM_011239558.2 | 49% | 52% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1224
- ORF length:
- 1152
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcagcc cagaatggaa acaccagttt cacacccaac tttaatccac 121 cccaagacca tgcctcctcc ctctccttta acttcagtta tggtgattat gacctcccta 181 tggatgagga tgaggacatg accaagaccc ggaccttctt cgcagccaag atcgtcattg 241 gcattgcact ggcaggcatc atgctggtct gcggcatcgg taactttgtc tttatcgctg 301 ccctcacccg ctataagaag ttgcgcaacc tcaccaatct gctcattgcc aacctggcca 361 tctccgactt cctggtggcc atcatctgct gccccttcga gatggactac tacgtggtac 421 ggcagctctc ctgggagcat ggccacgtgc tctgtgcctc cgtcaactac ctgcgcaccg 481 tctccctcta cgtctccacc aatgccttgc tggccattgc cattgacaga tatctcgcca 541 tcgttcaccc cttgaaacca cggatgaatt atcaaacggc ctccttcctg atcgccttgg 601 tctggatggt gtccattctc attgccatcc catcggctta ctttgcaaca gaaacggtcc 661 tctttattgt caagagccag gagaagatct tctgtggcca gatctggcct gtggatcagc 721 agctctacta caagtcctac ttcctcttca tctttggtgt cgagttcgtg ggccctgtgg 781 tcaccatgac cctgtgctat gccaggatct cccgggagct ctggttcaag gcagtccctg 841 ggttccagac ggagcagatt cgcaagcggc tgcgctgccg caggaagacg gtcctggtgc 901 tcatgtgcat tctcacggcc tatgtgctgt gctgggcacc cTTCTACGGT TTCACCATCG 961 TTCGTGACTT CTTCCCCACT GTGTTCGTGA AGGAAAAGCA CTACCTCACT GCCTTCTACG 1021 TGGTCGAGTG CATCGCCATG AGCAACAGCA TGATCAACAC CGTGTGCTTC GTGACGGTCA 1081 AGAACAACAC CATGAAGTAC TTCAAGAAGA TGATGCTGCT GCACTGGCGT CCCTCCCAGC 1141 GGGGGAGCAA GTCCAGTGCT GACCTTGACC TCAGAACCAA CGGGGTGCCC ACCACAGAAG 1201 AGGTGGACTG TATCAGGCTG AAGTAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1261 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1321 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAGTAATA 1381 TTACAGGTAT ATAGTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1441 aagatt