Transcript: Human NM_144773.3

Homo sapiens prokineticin receptor 2 (PROKR2), mRNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
PROKR2 (128674)
Length:
2242
CDS:
248..1402

Additional Resources:

NCBI RefSeq record:
NM_144773.3
NBCI Gene record:
PROKR2 (128674)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_144773.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000367766 TTGAAACCACGGATGAATTAT pLKO_005 728 CDS 100% 15.000 21.000 N PROKR2 n/a
2 TRCN0000008915 GCAACAGAAACGGTCCTCTTT pLKO.1 821 CDS 100% 4.950 6.930 N PROKR2 n/a
3 TRCN0000357268 ACTTCAGTTATGGTGATTATG pLKO_005 327 CDS 100% 13.200 9.240 N PROKR2 n/a
4 TRCN0000357267 CCATCCCATCGGCTTACTTTG pLKO_005 801 CDS 100% 10.800 7.560 N PROKR2 n/a
5 TRCN0000008912 CCATTCTCATTGCCATCCCAT pLKO.1 789 CDS 100% 2.640 1.848 N PROKR2 n/a
6 TRCN0000008911 GCCAAGATCGTCATTGGCATT pLKO.1 401 CDS 100% 0.405 0.284 N PROKR2 n/a
7 TRCN0000008913 CCTTGAAACCACGGATGAATT pLKO.1 726 CDS 100% 0.000 0.000 N PROKR2 n/a
8 TRCN0000008914 CCTCTCCTTTAACTTCAGTTA pLKO.1 316 CDS 100% 4.950 2.970 N PROKR2 n/a
9 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 2008 3UTR 100% 4.950 2.475 Y ORAI2 n/a
10 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 2005 3UTR 100% 4.950 2.475 Y LOC339059 n/a
11 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 1932 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_144773.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488075 AAGTAATATTACAGGTATATAGTC pLX_317 25% 100% 100% V5 (not translated due to prior stop codon) n/a
2 TRCN0000488657 ACATACCACTAATGTCTGGCCCCT pLX_317 24.8% 99.9% 99.7% V5 1152_1153insG n/a
3 ccsbBroadEn_09515 pDONR223 100% 99.9% 99.7% None 978C>A n/a
4 ccsbBroad304_09515 pLX_304 0% 99.9% 99.7% V5 978C>A n/a
5 TRCN0000477022 TTCCGGGCGCACGTCCCTTTAAGT pLX_317 24.6% 99.9% 99.7% V5 978C>A n/a
6 ccsbBroadEn_02548 pDONR223 100% 82.5% 84.9% None (many diffs) n/a
7 ccsbBroad304_02548 pLX_304 0% 82.5% 84.9% V5 (many diffs) n/a
8 TRCN0000478700 CATAAGCCTGCGCGCTGCCTCGCC pLX_317 26.1% 82.5% 84.9% V5 (many diffs) n/a
9 TRCN0000487968 TTCCTCTGCTACCCATACGGTGAC pLX_317 24.5% 82.4% 84.9% V5 (not translated due to prior stop codon) (many diffs) n/a
10 TRCN0000488184 ATTCCCAAAGGTGCGGGATCGAAG pLX_317 26% 82.3% 84.7% V5 (many diffs) n/a
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