Construct: ORF TRCN0000488078
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020432.1_s317c1
- DNA Barcode:
- GCATTTTTACTGCACTATGGCCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SRPK3 (26576)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488078
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26576 | SRPK3 | SRSF protein kinase 3 | NM_001170760.2 | 100% | 100% | |
| 2 | human | 26576 | SRPK3 | SRSF protein kinase 3 | NM_014370.4 | 99.8% | 99.8% | 774_776delGCA |
| 3 | human | 26576 | SRPK3 | SRSF protein kinase 3 | NM_001170761.1 | 94.1% | 94.1% | 948_949ins99 |
| 4 | mouse | 56504 | Srpk3 | serine/arginine-rich protei... | NM_019684.2 | 85.8% | 94.3% | (many diffs) |
| 5 | mouse | 56504 | Srpk3 | serine/arginine-rich protei... | XM_006528172.3 | 82.1% | 90.3% | (many diffs) |
| 6 | mouse | 56504 | Srpk3 | serine/arginine-rich protei... | XM_017318579.1 | 38% | 40.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1770
- ORF length:
- 1698
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagcgcc agcacgggcg gtggtgggga cagcggcggc agcggcggca 121 gtagcagcag ctcacaggcc tcctgcgggc ccgagtcctc gggctccgaa ctagccctgg 181 ccacaccggt gcctcagatg ctgcagggcc ttctgggctc cgacgacgag gaacaggaag 241 accccaaaga ctactgcaag ggcggctacc accctgtgaa gatcggcgac gtgttcaatg 301 ggcggtacca cgtggtgcgc aaactgggct ggggccactt ctccaccgtc tggctctgct 361 gggacatcca gcgcaagcgc tttgtggccc tcaaagtggt gaagagtgcg gggcattaca 421 cggagacagc tgtggatgag atcaagctcc tgaaatgtgt ccgggacagc gaccccagtg 481 accccaaaag agagaccatt gtccagctca ttgatgactt caggatctca ggagtcaatg 541 gagtccatgt gtgcatggtg ctggaggtgc tgggccacca gctcctcaaa tggatcatca 601 agtccaacta ccagggcctg cccgtgccct gcgtgaagag catcgtgagg caggtgctgc 661 acggcctgga ctacctccac accaagtgca agatcatcca cacggacatc aagcccgaga 721 acatcttgct gtgtgtgggg gacgcttaca tcaggcgcct ggctgccgag gccacggagt 781 ggcaacaggc aggggcgccg cccccctccc gctccatagt cagcactgcc ccccaggagg 841 tcttgaccgg taagctgtcc aaaaacaaga ggaagaagat gaggcgcaaa cggaaacagc 901 agaagcggct gctggaggag cggctgcggg acctgcagag gctggaggcc atggaggctg 961 ccacccaggc tgaggactct ggcttgagac tagacggggg cagcggctcc acatcctctt 1021 caggctgtca ccccgggggc gccagagcag gtccctcccc agcctcttcc tcccccgccc 1081 cagggggcgg ccgtagcctc agcgcgggct cacagacctc aggcttctcc ggctccctct 1141 tctctcctgc ctcctgctcc atcctctccg gctcgtccaa tcagcgagag accgggggcc 1201 tcctgtcgcc tagcacacca ttcggtgcct cgaacctcct ggtgaacccc ctggagcccc 1261 aaaatgcaga taagatcaag atcaagatcg cagacctggg caacgcctgc tgggtgcaca 1321 agcacttcac ggaagacatc cagactcggc agtaccgggc cgtcgaggtg ctgatcggcg 1381 ccgaatacgg ccccccggca gacatctgga gcacagcctg catggccttc gagctggcca 1441 ctggtgacta cctgttcgag ccgcattctg gagaagacta cagtcgtgat gaggaccaca 1501 tcgctcacat agtggagctt ctgggggaca tccccccagc cTTCGCCCTC TCAGGCCGCT 1561 ATTCCCGGGA GTTCTTCAAC CGGAGAGGAG AGCTGCGGCA CATCCACAAT CTCAAGCACT 1621 GGGGCCTGTA CGAGGTACTC ATGGAAAAGT ACGAGTGGCC CCTAGAGCAG GCCACACAGT 1681 TCAGCGCCTT TCTGCTGCCC ATGATGGAGT ACATCCCCGA AAAGCGGGCC AGTGCCGCTG 1741 ACTGCCTCCA GCACCCCTGG CTCAACCCCT AGAAGCTTGC CCAGCTTTCT TGTACAAAGT 1801 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 1861 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 1921 CGAGCATTTT TACTGCACTA TGGCCTTACG CGTTAAGTCg acaatcaacc tctggattac 1981 aaaatttgtg aaagatt