Construct: ORF TRCN0000488094
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020462.1_s317c1
- DNA Barcode:
- ACGAATCAGCTTAATCGTCTTCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K5 (4217)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488094
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4217 | MAP3K5 | mitogen-activated protein k... | NM_005923.4 | 99.9% | 100% | 1557A>G |
| 2 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010873.1 | 92.6% | 92.6% | 1_327del;1884A>G |
| 3 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010872.1 | 92.5% | 92.5% | 1_327del;1884A>G;3205_3207delGCA |
| 4 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010871.1 | 91.2% | 90.6% | (many diffs) |
| 5 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010870.1 | 91.1% | 90.5% | (many diffs) |
| 6 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010875.1 | 90.2% | 90.2% | 1_327del;1884A>G;2007_2008ins108 |
| 7 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010874.1 | 88.7% | 88.1% | (many diffs) |
| 8 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010877.1 | 85.7% | 85.6% | (many diffs) |
| 9 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010876.1 | 84.3% | 83.5% | (many diffs) |
| 10 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_011535839.3 | 79.1% | 79% | (many diffs) |
| 11 | mouse | 26408 | Map3k5 | mitogen-activated protein k... | NM_008580.4 | 86.4% | 94.2% | (many diffs) |
| 12 | mouse | 26408 | Map3k5 | mitogen-activated protein k... | XM_006512739.3 | 65% | 65.9% | (many diffs) |
| 13 | mouse | 26408 | Map3k5 | mitogen-activated protein k... | XR_001779529.1 | 46.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 4194
- ORF length:
- 4122
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagcacg gaggcggacg agggcatcac tttctctgtg ccacccttcg 121 ccccctcggg cttctgcacc atccccgagg gcggcatctg caggagggga ggagcggcgg 181 cggtgggcga gggcgaggag caccagctgc caccgccgcc gccgggcagc ttctggaacg 241 tggagagcgc cgctgcccct ggcatcggtt gtccggcggc cacctcctcg agcagtgcca 301 cccgaggccg gggcagctct gttggcgggg gcagccgacg gaccacggtg gcatatgtga 361 tcaacgaagc gagccaaggg caactggtgg tggccgagag cgaggccctg cagagcttgc 421 gggaggcgtg cgagacagtg ggcgccaccc tggaaaccct gcattttggg aaactcgact 481 ttggagaaac caccgtgctg gaccgctttt acaatgcaga tattgcggtg gtggagatga 541 gcgatgcctt ccggcagccg tccttgtttt accaccttgg ggtgagagaa agtttcagca 601 tggccaacaa catcatcctc tactgtgata ctaactcgga ctctctgcag tcactgaagg 661 aaataatttg ccagaagaat actatgtgca ctgggaacta cacctttgtt ccttacatga 721 taactccaca taacaaagtc tactgctgtg acagcagctt catgaagggg ttgacagagc 781 tcatgcaacc gaacttcgag ctgcttcttg gacccatctg cttacctctt gtggatcgtt 841 ttattcaact tttgaaggtg gcacaagcaa gttctagcca gtacttccgg gaatctatac 901 tcaatgacat caggaaagct cgtaatttat acactggtaa agaattggca gctgagttgg 961 caagaattcg gcagcgagta gataatatcg aagtcttgac agcagatatt gtcataaatc 1021 tgttactttc ctacagagat atccaggact atgattctat tgtgaagctg gtagagactt 1081 tagaaaaact gccaaccttt gatttggcct cccatcacca tgtgaagttt cattatgcat 1141 ttgcactgaa taggagaaat ctccctggtg acagagcaaa agctcttgat attatgattc 1201 ccatggtgca aagcgaagga caagttgctt cagatatgta ttgcctagtt ggtcgaatct 1261 acaaagatat gtttttggac tctaatttca cggacactga aagcagagac catggagctt 1321 cttggttcaa aaaggcattt gaatctgagc caacactaca gtcaggaatt aattatgcgg 1381 tcctcctcct ggcagctgga caccagtttg aatcttcctt tgagctccgg aaagttgggg 1441 tgaagctaag tagtcttctt ggtaaaaagg gaaacttgga aaaactccag agctactggg 1501 aagttggatt ttttctgggg gccagcgtcc tagccaatga ccacatgaga gtcattcaag 1561 catctgaaaa gctttttaaa ctgaagacac cagcatggta cctcaagtct attgtagaga 1621 caattttgat atataagcat tttgtgaaac tgaccacaga acagcctgtg gccaagcaag 1681 aacttgtgga cttttggatg gatttcctgg tcgaggccac aaagacagat gttactgtgg 1741 ttaggtttcc agtattaata ttagaaccaa ccaaaatcta tcaaccttct tatttgtcta 1801 tcaacaatga agttgaggaa aagacaatct ctatttggca cgtgcttcct gatgacaaga 1861 aaggtataca tgagtggaat tttagtgcct cttctgtcag gggagtgagt atttctaaat 1921 ttgaagaaag atgctgcttt ctttatgtgc ttcacaattc tgatgatttc caaatctatt 1981 tctgtacaga acttcattgt aaaaagtttt ttgagatggt gaacaccatt accgaagaga 2041 aggggagaag cacagaggaa ggagactgtg aaagtgactt gctggagtat gactatgaat 2101 atgatgaaaa tggtgacaga gtcgttttag gaaaaggcac ttatgggata gtctacgcag 2161 gtcgggactt gagcaaccaa gtcagaattg ctattaagga aatcccagag agagacagca 2221 gatactctca gcccctgcat gaagaaatag cattgcataa acacctgaag cacaaaaata 2281 ttgtccagta tctgggctct ttcagtgaga atggtttcat taaaatcttc atggagcagg 2341 tccctggagg aagtctttct gctctccttc gttccaaatg gggtccatta aaagacaatg 2401 agcaaacaat tggcttttat acaaagcaaa tactggaagg attaaaatat ctccatgaca 2461 atcagatagt tcaccgggac ataaagggtg acaatgtgtt gattaatacc tacagtggtg 2521 ttctcaagat ctctgacttc ggaacatcaa agaggcttgc tggcataaac ccctgtactg 2581 aaacttttac tggtaccctc cagtatatgg caccagaaat aatagataaa ggaccaagag 2641 gctacggaaa agcagcagac atctggtctc tgggctgtac aatcattgaa atggccacag 2701 gaaaaccccc attttatgaa ctgggagaac cacaagcagc tatgttcaag gtgggaatgt 2761 ttaaagtcca ccctgagatc ccagagtcca tgtctgcaga ggccaaggca ttcatactga 2821 aatgttttga accagatcct gacaagagag cctgtgctaa cgacttgctt gttgatgagt 2881 ttttaaaagt ttcaagcaaa aagaaaaaga cacaacctaa gctttcagct ctttcagctg 2941 gatcaaatga atatctcagg agtatatcct tgccggtacc tgtgctggtg gaggacacca 3001 gcagcagcag tgagtacggc tcagtttcac ccgacacgga gttgaaagtg gaccccttct 3061 ctttcaaaac aagagccaag tcctgcggag aaagagatgt caagggaatt cggacactct 3121 ttttgggcat tccagatgag aattttgaag atcacagtgc tcctccttcc cctgaagaaa 3181 aagattctgg attcttcatg ctgaggaagg acagtgagag gcgagctacc cttcacagga 3241 tcctgacgga agaccaagac aaaattgtga gaaacctaat ggaatcttta gctcaggggg 3301 ctgaagaacc gaaactaaaa tgggaacaca tcacaaccct cattgcaagc ctcagagaat 3361 ttgtgagatc cactgaccga aaaatcatag ccaccacact gtcaaagctg aaactggagc 3421 tggacttcga cagccatggc attagccaag tccaggtggt actctttggt tttcaagatg 3481 ctgtcaataa agttcttcgg aatcataaca tcaagccgca ctggatgttt gccttagaca 3541 gtatcattcg gaaggcggta cagacagcca ttaccatcct ggttccagaa ctaaggccac 3601 atttcagcct tgcatctgag agtgatactg ctgatcaaga agacttggat gtagaagatg 3661 accatgagga acagccttca aatcaaactg tccgaagacc tcaggctgtc attgaagatg 3721 ctgtggctac ctcaggcgtg agcacgctca gttctactgt gtctcatgat tcccagagtg 3781 ctcaccggtc actgaatgta cagcttggaa ggatgaaaat agaaaccaat agattactgg 3841 aagaattggt tcggaaagag aaagaattac aagcactcct tcatcgagct attGAAGAAA 3901 AAGACCAAGA AATTAAACAC CTGAAGCTTA AGTCCCAACC CATAGAAATT CCTGAATTGC 3961 CTGTATTTCA TCTAAATTCT TCTGGCACAA ATACTGAAGA TTCTGAACTT ACCGACTGGC 4021 TGAGAGTGAA TGGAGCTGAT GAAGACACTA TAAGCCGGTT TTTGGCTGAA GATTATACAC 4081 TATTGGATGT TCTCTACTAT GTTACACGTG ATGACTTAAA ATGCTTGAGA CTAAGGGGAG 4141 GGATGCTGTG CACACTGTGG AAGGCTATCA TTGACTTTCG AAACAAACAG ACTTAGGACC 4201 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 4261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 4321 ATATATCTTG TGGAAAGGAC GAACGAATCA GCTTAATCGT CTTCTCACGC GTTAAGTCga 4381 caatcaacct ctggattaca aaatttgtga aagatt