Construct: ORF TRCN0000488119
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019452.1_s317c1
- DNA Barcode:
- TAGTATTCGCCAATATCTCACGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MOXD1 (26002)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488119
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 26002 | MOXD1 | monooxygenase DBH like 1 | XM_017010714.2 | 93.3% | 89.9% | (many diffs) |
2 | human | 26002 | MOXD1 | monooxygenase DBH like 1 | NM_015529.4 | 88.6% | 88.5% | 1_207del;1839_1840insG |
3 | mouse | 59012 | Moxd1 | monooxygenase, DBH-like 1 | NM_021509.5 | 74.8% | 76.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 65
- ORF end:
- 1700
- ORF length:
- 1635
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacatt 61 gaccatggcg tccgccgaca tcgtcgtggg cggggtggcc cacgggcggc cctacctcca 121 ggattatttt acaaatgcaa atagagagtt gaaaaaagat gctcagcaag attaccatct 181 agaatatgcc atggaaaata gcacacacac aataattgaa tttaccagag agctgcatac 241 atgtgacata aatgacaaga gtataacgga tagcactgtg agagtgatct gggcctacca 301 ccatgaagat gcaggagaag ctggtcccaa gtaccatgac tccaataggg gcaccaagag 361 tttgcggtta ttgaatcctg agaaaactag tgtgctatct acagccttac catactttga 421 tctggtaaat caggacgtcc ccatcccaaa caaagataca acatattggt gccaaatgtt 481 taagattcct gtgttccaag aaaagcatca tgtaataaag gttgagccag tgatacagag 541 aggccatgag agtctggtgc accacatcct gctctatcag tgcagcaaca actttaacga 601 cagcgttctg gagtccggcc acgagtgcta tcaccccaac atgcccgatg cattcctcac 661 ctgtgaaact gtgatttttg cctgggctat tggtggagag ggcttttctt atccacctca 721 tgttggatta tcccttggca ctccattaga tccgcattat gtgctcctag aagtccatta 781 tgataatccc acttatgagg aaggcttaat agataattct ggactgaggt tattttacac 841 aatggatata aggaaatatg atgctggggt gattgaggct ggcctctggg tgagcctctt 901 ccataccatc cctccaggga tgcctgagtt ccagtctgag ggtcactgca ctttggagtg 961 cctggaagag gctctggaag ccgaaaagcc aagtggaatt catgtgtttg ctgttcttct 1021 ccatgctcac ctggctggca gaggcatcag gctgcgtcat tttcgaaaag ggaaggaaat 1081 gaaattactt gcctatgatg atgattttga cttcaatttc caggagtttc agtatctaaa 1141 ggaagaacaa acaatcttac caggagataa cctaattact gagtgtcgct acaacacgaa 1201 agatagagct gagatgactt ggggaggact aagcaccagg agtgaaatgt gtctctcata 1261 ccttctttat tacccaagaa ttaatcttac tcgatgtgca agtattccag acattatgga 1321 acaacttcag ttcattgggg ttaaggagat ctacagacca gtcacgacct ggcctttcat 1381 tatcaaaagt cccaagcaat ataaaaaCCT TTCTTTCATG GATGCTATGA ATAAGTTTAA 1441 ATGGACTAAA AAGGAAGGTC TCTCCTTCAA CAAGCTGGTC CTCAGCCTGC CAGTGAATGT 1501 GAGATGTTCC AAGACAGACA ATGCTGAGTG GTCGATTCAA GGAATGACAG CATTACCTCC 1561 AGATATAGAA AGACCCTATA AAGCAGAACC TTTGGTGTGT GGCACGTCTT CTTCCTCTTC 1621 CCTGCACAGA GATTTCTCCA TCAACTTGCT TGTTTGCCTT CTGCTACTCA GCTGCACGCT 1681 GAGCACCAAG AGCTTGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 1741 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 1801 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATAGTATTC GCCAATATCT 1861 CACGGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt