Construct: ORF TRCN0000488140
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021121.1_s317c1
- DNA Barcode:
- TTTCTCTGCTGCAAACACCTTCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LTB4R2 (56413)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488140
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56413 | LTB4R2 | leukotriene B4 receptor 2 | NM_001164692.2 | 92% | 92% | 0_1ins93 |
| 2 | human | 56413 | LTB4R2 | leukotriene B4 receptor 2 | NM_019839.5 | 92% | 92% | 0_1ins93 |
| 3 | mouse | 57260 | Ltb4r2 | leukotriene B4 receptor 2 | XM_006519323.3 | 80.7% | 85.4% | (many diffs) |
| 4 | mouse | 57260 | Ltb4r2 | leukotriene B4 receptor 2 | XM_006519325.3 | 80.7% | 85.4% | (many diffs) |
| 5 | mouse | 57260 | Ltb4r2 | leukotriene B4 receptor 2 | XM_017316100.1 | 80.7% | 85.4% | (many diffs) |
| 6 | mouse | 57260 | Ltb4r2 | leukotriene B4 receptor 2 | NM_020490.2 | 78.4% | 84.4% | (many diffs) |
| 7 | mouse | 57260 | Ltb4r2 | leukotriene B4 receptor 2 | XM_006519326.3 | 78.4% | 84.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1239
- ORF length:
- 1167
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcacct tctcatcggg catcacaggt ggggttttgc cccacccctg 121 aacgccctct gtggcgcctt ccacccacct gtaggcccag aaggatgtcg gtctgctacc 181 gtcccccagg gaacgagaca ctgctgagct ggaagacttc gcgggccaca ggcacagcct 241 tcctgctgct ggcggcgctg ctggggctgc ctggcaacgg cttcgtggtg tggagcttgg 301 cgggctggcg gcctgcacgg gggcgaccgc tggcggccac gcttgtgctg cacctggcgc 361 tggccgacgg cgcggtgctg ctgctcacgc cgctctttgt ggccttcctg acccggcagg 421 cctggccgct gggccaggcg ggctgcaagg cggtgtacta cgtgtgcgcg ctcagcatgt 481 acgccagcgt gctgctcacc ggcctgctca gcctgcagcg ctgcctcgca gtcacccgcc 541 ccttcctggc gcctcggctg cgcagcccgg ccctggcccg ccgcctgctg ctggcggtct 601 ggctggccgc cctgttgctc gccgtcccgg ccgccgtcta ccgccacctg tggagggacc 661 gcgtatgcca gctgtgccac ccgtcgccgg tccacgccgc cgcccacctg agcctggaga 721 ctctgaccgc tttcgtgctt cctttcgggc tgatgctcgg ctgctacagc gtgacgctgg 781 cacggctgcg gggcgcccgc tggggctccg ggcggcacgg ggcgcgggtg ggccggctgg 841 tgagcgccat cgtgcttgcc ttcggcttgc tctgggcccc ctaccacgca gtcaaccttc 901 tgcaggcggt cgcagcgctg gctccaccgg aaggggcctt ggcgaagctg ggcggagccg 961 gccaggcggc gcgagcggga actacggcct tggccTTCTT CAGTTCTAGC GTCAACCCGG 1021 TGCTCTACGT CTTCACCGCT GGAGATCTGC TGCCCCGGGC AGGTCCCCGT TTCCTCACGC 1081 GGCTCTTCGA AGGCTCTGGG GAGGCCCGAG GGGGCGGCCG CTCTAGGGAA GGGACCATGG 1141 AGCTCCGAAC TACCCCTCAG CTGAAAGTGG TGGGGCAGGG CCGCGGCAAT GGAGACCCGG 1201 GGGGTGGGAT GGAGAAGGAC GGTCCGGAAT GGGACCTTTG AGACCCAGCT TTCTTGTACA 1261 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1321 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1381 AGGACGATTT CTCTGCTGCA AACACCTTCA CACGCGTTAA GTCgacaatc aacctctgga 1441 ttacaaaatt tgtgaaagat t