Construct: ORF TRCN0000488159

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021021.1_s317c1
DNA Barcode:
AAAGAGACGACCAAAAGTACATGC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
ADGRE3 (84658)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488159
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84658 ADGRE3 adhesion G protein-coupled ... NM_032571.5 99.9% 99.8% 1154G>A
2 human 84658 ADGRE3 adhesion G protein-coupled ... XM_011528374.2 97.3% 97% 25_26ins51;1103G>A
3 human 84658 ADGRE3 adhesion G protein-coupled ... NM_001289158.1 91.9% 91.8% 198_199ins156;998G>A
4 human 84658 ADGRE3 adhesion G protein-coupled ... XR_001753772.1 85% 1_79del;1233G>A;2036_2300del
5 human 84658 ADGRE3 adhesion G protein-coupled ... NM_001289159.2 80.6% 80.5% 198_199ins378;776G>A
6 human 84658 ADGRE3 adhesion G protein-coupled ... XM_017027383.1 66.9% 66.8% 0_1ins645;509G>A
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2028
ORF length:
1956
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgcaggga ccattgcttc ttccaggcct ctgctttctg ctgagcctct
121ttggagctgt gactcagaaa accaaaactt cctgtgctaa gtgcccccca aatgcttcct
181gtgtcaataa cactcactgc acctgcaacc atggatatac ttctggatct gggcagaaac
241tattcacatt ccccttggag acatgtaacg acattaatga atgtacacca ccctatagtg
301tatattgtgg atttaacgct gtgtgttaca atgtcgaagg aagtttctac tgtcaatgtg
361tcccaggata tagactgcat tctgggaatg aacaattcag taattccaat gagaacacct
421gtcaggacac cacctcctca aagacaaccg agggcaggaa agagctgcaa aagattgtgg
481acaaatttga gtcacttctc accaatcaga ctttatggag aacagaaggg agacaagaaa
541tctcatccac agctaccact attctccggg atgtggaatc gaaagttcta gaaactgcct
601tgaaagatcc agaacaaaaa gtcctgaaaa tccaaaacga tagtgtagct attgaaactc
661aagcgattac agacaattgc tctgaagaaa gaaagacatt caacttgaac gtccaaatga
721actcaatgga catccgttgc agtgacatca tccagggaga cacacaaggt cccagtgcca
781ttgcctttat ctcatattct tctcttggaa acatcataaa tgcaactttt tttgaagaga
841tggataagaa agatcaagtg tatctgaact ctcaggttgt gagtgctgct attggaccca
901aaaggaacgt gtctctctcc aagtctgtga cgctgacttt ccagcacgtg aagatgaccc
961ccagtaccaa aaaggtcttc tgtgtctact ggaagagcac agggcagggc agccagtggt
1021ccagggatgg ctgcttcctg atacacgtga acaagagtca caccatgtgt aattgcagtc
1081acctgtccag cttcgctgtc ctgatggccc tgaccagcca ggaggaggat cccgtgctga
1141ctgtcatcac ctacgtgggg ctgagcgtct ctctgctgtg cctcctcctg gcggccctca
1201cttttctcct gtgtaaagcc atccagaaca ccagcacctc actgcatctg cagctctcgc
1261tctgcctctt cctggcccac ctcctcttcc tcgtggggat tgatcgaact gaacccaagg
1321tgctgtgctc catcatcgcc ggtgctttgc actatctcta cctggccgcc ttcacctgga
1381tgctgctgga gggtgtgcac ctcttcctca ctgcacggaa cctgacagtg gtcaactact
1441caagcatcaa tagactcatg aagtggatca tgttcccagt cggctatggc gttcccgctg
1501tgactgtggc catttctgca gcctcctggc ctcaccttta tggaactgct gatcgatgct
1561ggctccacct ggaccaggga ttcatgtgga gtttccttgg cccagtctgt gccattttct
1621ctgcgaattt agtattgttt atcttggtct tttggatttt gaaaagaaaa ctttccTCCC
1681TCAATAGTGA AGTGTCAACC ATCCAGAACA CAAGGATGCT GGCTTTCAAA GCAACAGCTC
1741AGCTCTTCAT CCTGGGCTGC ACATGGTGTC TGGGCTTGCT ACAGGTGGGT CCAGCTGCCC
1801AGGTCATGGC CTACCTCTTC ACCATCATCA ACAGCCTCCA AGGCTTCTTC ATCTTCTTGG
1861TCTACTGCCT CCTCAGCCAG CAGGTCCAGA AACAATATCA AAAGTGGTTT AGAGAGATCG
1921TAAAATCAAA ATCTGAGTCT GAGACATACA CACTTTCCAG CAAGATGGGT CCTGACTCAA
1981AACCCAGTGA GGGGGATGTT TTTCCAGGAC AAGTGAAGAG AAAATATTAG AACCCAGCTT
2041TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
2101CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
2161CTTGTGGAAA GGACGAAAAG AGACGACCAA AAGTACATGC ACGCGTTAAG TCgacaatca
2221acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)