Construct: ORF TRCN0000488160
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020667.1_s317c1
- DNA Barcode:
- AGCACATACATTTTTCCTTCAGTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EPHA3 (2042)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488160
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2042 | EPHA3 | EPH receptor A3 | NM_005233.6 | 100% | 100% | |
| 2 | human | 2042 | EPHA3 | EPH receptor A3 | XM_005264715.3 | 99.8% | 99.8% | 1429_1430insAGC |
| 3 | human | 2042 | EPHA3 | EPH receptor A3 | XM_005264716.3 | 93.3% | 93.1% | 2748_2749insG;2754_2754delAins195 |
| 4 | human | 2042 | EPHA3 | EPH receptor A3 | NM_182644.3 | 54.6% | 53.9% | (many diffs) |
| 5 | mouse | 13837 | Epha3 | Eph receptor A3 | NM_010140.3 | 88.7% | 96.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3021
- ORF length:
- 2949
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggattgt cagctctcca tcctcctcct tctcagctgc tctgttctcg 121 acagcttcgg ggaactgatt ccgcagcctt ccaatgaagt caatctactg gattcaaaaa 181 caattcaagg ggagctgggc tggatctctt atccatcaca tgggtgggaa gagatcagtg 241 gtgtggatga acattacaca cccatcagga cttaccaggt gtgcaatgtc atggaccaca 301 gtcaaaacaa ttggctgaga acaaactggg tccccaggaa ctcagctcag aagatttatg 361 tggagctcaa gttcactcta cgagactgca atagcattcc attggtttta ggaacttgca 421 aggagacatt caacctgtac tacatggagt ctgatgatga tcatggggtg aaatttcgag 481 agcatcagtt tacaaagatt gacaccattg cagctgatga aagtttcact caaatggatc 541 ttggggaccg tattctgaag ctcaacactg agattagaga agtaggtcct gtcaacaaga 601 agggatttta tttggcattt caagatgttg gtgcttgtgt tgccttggtg tctgtgagag 661 tatacttcaa aaagtgccca tttacagtga agaatctggc tatgtttcca gacacggtac 721 ccatggactc ccagtccctg gtggaggtta gagggtcttg tgtcaacaat tctaaggagg 781 aagatcctcc aaggatgtac tgcagtacag aaggcgaatg gcttgtaccc attggcaagt 841 gttcctgcaa tgctggctat gaagaaagag gttttatgtg ccaagcttgt cgaccaggtt 901 tctacaaggc attggatggt aatatgaagt gtgctaagtg cccgcctcac agttctactc 961 aggaagatgg ttcaatgaac tgcaggtgtg agaataatta cttccgggca gacaaagacc 1021 ctccatccat ggcttgtacc cgacctccat cttcaccaag aaatgttatc tctaatataa 1081 acgagacctc agttatcctg gactggagtt ggcccctgga cacaggaggc cggaaagatg 1141 ttaccttcaa catcatatgt aaaaaatgtg ggtggaatat aaaacagtgt gagccatgca 1201 gcccaaatgt ccgcttcctc cctcgacagt ttggactcac caacaccacg gtgacagtga 1261 cagaccttct ggcacatact aactacacct ttgagattga tgccgttaat ggggtgtcag 1321 agctgagctc cccaccaaga cagtttgctg cggtcagcat cacaactaat caggctgctc 1381 catcacctgt cctgacgatt aagaaagatc ggacctccag aaatagcatc tctttgtcct 1441 ggcaagaacc tgaacatcct aatgggatca tattggacta cgaggtcaaa tactatgaaa 1501 agcaggaaca agaaacaagt tataccattc tgagggcaag aggcacaaat gttaccatca 1561 gtagcctcaa gcctgacact atatacgtat tccaaatccg agcccgaaca gccgctggat 1621 atgggacgaa cagccgcaag tttgagtttg aaactagtcc agactctttc tccatctctg 1681 gtgaaagtag ccaagtggtc atgatcgcca tttcagcggc agtagcaatt attctcctca 1741 ctgttgtcat ctatgttttg attgggaggt tctgtggcta taagtcaaaa catggggcag 1801 atgaaaaaag acttcatttt ggcaatgggc atttaaaact tccaggtctc aggacttatg 1861 ttgacccaca tacatatgaa gaccctaccc aagctgttca tgagtttgcc aaggaattgg 1921 atgccaccaa catatccatt gataaagttg ttggagcagg tgaatttgga gaggtgtgca 1981 gtggtcgctt aaaacttcct tcaaaaaaag agatttcagt ggccattaag accctgaaag 2041 ttggctacac agaaaagcag aggagagact tcctgggaga agcaagcatt atgggacagt 2101 ttgaccaccc caatatcatt cgactggaag gagttgttac caaaagtaag ccagttatga 2161 ttgtcacaga atacatggag aatggttcct tggatagttt cctacgtaaa cacgatgccc 2221 agtttactgt cattcagcta gtggggatgc ttcgagggat agcatctggc atgaagtacc 2281 tgtcagacat gggctatgtt caccgagacc tcgctgctcg gaacatcttg atcaacagta 2341 acttggtgtg taaggtttct gatttcggac tttcgcgtgt cctggaggat gacccagaag 2401 ctgcttatac aacaagagga gggaagatcc caatcaggtg gacatcacca gaagctatag 2461 cctaccgcaa gttcacgtca gccagcgatg tatggagtta tgggattgtt ctctgggagg 2521 tgatgtctta tggagagaga ccatactggg agatgtccaa tcaggatgta attaaagctg 2581 tagatgaggg ctatcgactg ccacccccca tggactgccc agctgccttg tatcagctga 2641 tgctggactg ctggcagaaa gacaggaaca acagacccaa gtttgagcag attgttagta 2701 ttctggacaa gcttatccgg aatcccggca gcctgaagat catcaccagt gcagccgcaa 2761 ggccatcaaa ccttcttctg gaccaaagca atgtggatat cactaCCTTC CGCACAACAG 2821 GTGACTGGCT TAATGGTGTC TGGACAGCAC ACTGCAAGGA AATCTTCACG GGTGTGGAGT 2881 ACAGTTCTTG TGACACAATA GCCAAGATTT CCACAGATGA CATGAAAAAG GTTGGTGTCA 2941 CCGTGGTTGG GCCACAGAAG AAGATCATCA GTAGCATTAA AGCTCTAGAA ACGCAATCAA 3001 AGAATGGCCC AGTTCCCGTG TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 3061 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 3121 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GCACATACAT 3181 TTTTCCTTCA GTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 3241 att