Construct: ORF TRCN0000488167
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019366.1_s317c1
- DNA Barcode:
- CAGGTCATATCCGCTCTCATTATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SBNO1 (55206)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488167
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55206 | SBNO1 | strawberry notch homolog 1 | NM_018183.5 | 20.1% | 20.1% | 1_3333del;3345T>C;4176_4177insG |
| 2 | human | 55206 | SBNO1 | strawberry notch homolog 1 | NM_001167856.3 | 20.1% | 20.1% | 1_3336del;3348T>C;4179_4180insG |
| 3 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530322.3 | 18.2% | 20.5% | (many diffs) |
| 4 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530323.3 | 18.2% | 20.5% | (many diffs) |
| 5 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530320.3 | 18.2% | 20.4% | (many diffs) |
| 6 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530321.3 | 18.2% | 20.4% | (many diffs) |
| 7 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530319.3 | 17.7% | 19.9% | (many diffs) |
| 8 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | NM_001081203.1 | 17.7% | 19.9% | (many diffs) |
| 9 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530317.3 | 17.7% | 19.9% | (many diffs) |
| 10 | mouse | 243272 | Sbno1 | strawberry notch homolog 1 ... | XM_006530318.3 | 17.7% | 19.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 918
- ORF length:
- 846
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaggtg caccagcaga atgcgttatt tcagtatttt gcggacacac 121 ttactgcagt tgttcaaaat gccaaaaaaa atggaagata tgatatggga atcttagatc 181 ttggttctgg agatgaaaaa gtgcggaaaa gtgatgttaa aaagtttctg actccaggat 241 attcaacctc tggccacgta gaattataca caattagtgt agagagggga atgtcatggg 301 aggaagctac caagatttgg gctgagctga caggaccaga cgatggcttt tacttgtcat 361 tgcaaataag gaacaacaag aaaactgcca tcttagttaa agaagtgaat cctaaaaaga 421 aacttttctt agtttatcga ccaaatactg ggaagcagct caaattagaa atttatgctg 481 atctaaaaaa gaaatataag aaggtcgtct cagatgatgc cctgatgcac tggttagatc 541 agtataattc atctgcagat acttgtactc atgcttattg gcgcggcaat tgcaaaaaag 601 caagcttGGG GCTAGTTTGT GAAATAGGTC TTCGTTGCCG TACATATTAT GTATTATGTG 661 GTTCAGTGCT GAGTGTCTGG ACAAAAGTTG AGGGTGTTCT AGCATCTGTC AGTGGCACAA 721 ACGTGAAGAT GCAGATCGTG CGGCTAAGAA CGGAAGATGG GCAACGGATT GTAGGTTTGA 781 TCATTCCGGC AAATTGTGTG TCTCCTCTTG TAAATCTCCT ATCAACTTCA GACCAGTCTC 841 AACAGCTTGC GGTCCAACAG AAACAGCTAT GGCAACAGCA TCACCCTCAG AGCATCACCA 901 ACTTGAGCAA CGCAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 961 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1021 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CAGGTCATAT CCGCTCTCAT 1081 TATGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt