Construct: ORF TRCN0000488180
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020005.1_s317c1
- DNA Barcode:
- CATTGTGGTCATTCTCCTCATTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDGFRL (5157)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488180
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5157 | PDGFRL | platelet derived growth fac... | NM_006207.2 | 99.7% | 99.7% | 999C>T;1041T>C;1125_1126insG |
| 2 | human | 5157 | PDGFRL | platelet derived growth fac... | NM_001372073.1 | 99.6% | 99.7% | (many diffs) |
| 3 | mouse | 68797 | Pdgfrl | platelet-derived growth fac... | NM_026840.3 | 83.9% | 89% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1200
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaaggtc tggctgctgc ttggtcttct gctggtgcac gaagcgctgg 121 aggatgttac tggccaacac cttcccaaga acaagcgtcc aaaagaacca ggagagaata 181 gaatcaaacc taccaacaag aaggtgaagc ccaaaattcc taaaatgaag gacagggact 241 cagccaattc agcaccaaag acgcagtcta tcatgatgca agtgctggat aaaggtcgct 301 tccagaaacc cgccgctacc ctgagtctgc tggcggggca aactgtagag cttcgatgta 361 aagggagtag aattgggtgg agctaccctg cgtatctgga cacctttaag gattctcgcc 421 tcagcgtcaa gcagaatgag cgctacggcc agttgactct ggtcaactcc acctcggcag 481 acacaggtga attcagctgc tgggtgcagc tctgcagcgg ctacatctgc aggaaggacg 541 aggccaaaac gggctccacc tacatctttt ttacagagaa aggagaactc tttgtacctt 601 ctcccagcta cttcgatgtt gtctacttga acccggacag acaggctgtg gttccttgtc 661 gggtgaccgt gctgtcggcc aaagtcacgc tccacaggga attcccagcc aaggagatcc 721 cagccaatgg aacggacatt gtttatgaca tgaagcgggg ctttgtgtat ctgcaacctc 781 attccgagca ccagggtgtg gtttactgca gggcggaggc cgggggcaga tctcagatct 841 ccgtcaagta ccagctgctc tacgtggcgg ttcccagtgg ccctccctca acaaccatct 901 tGGCTTCTTC AAACAAAGTG AAAAGTGGGG ACGACATCAG TGTGCTCTGC ACTGTCCTGG 961 GGGAGCCCGA TGTGGAGGTG GAGTTCACCT GGATCTTCCC AGGGCAGAAG GATGAAAGGC 1021 CTGTGACGAT CCAAGACACT TGGAGGTTGA TCCACAGAGG ACTGGGACAT ACCACGAGAA 1081 TCTCCCAGAG TGTCATTACA GTGGAAGACT TCGAGACGAT TGATGCAGGA TATTACATTT 1141 GCACTGCTCA GAATCTTCAA GGACAGACCA CAGTAGCTAC CACTGTTGAG TTTTCCGACC 1201 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GACATTGTGG TCATTCTCCT CATTTAACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt