Construct: ORF TRCN0000488193
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021184.1_s317c1
- DNA Barcode:
- TACACGCAAGGTTGAGCTTGGCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LHCGR (3973)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488193
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3973 | LHCGR | luteinizing hormone/choriog... | NM_000233.4 | 100% | 100% | |
| 2 | human | 3973 | LHCGR | luteinizing hormone/choriog... | XM_017004089.1 | 80.7% | 78.6% | (many diffs) |
| 3 | human | 3973 | LHCGR | luteinizing hormone/choriog... | XM_011532831.1 | 69.6% | 69.6% | 0_1ins636 |
| 4 | human | 3973 | LHCGR | luteinizing hormone/choriog... | XM_017004090.1 | 69.6% | 69.6% | 0_1ins636 |
| 5 | human | 3973 | LHCGR | luteinizing hormone/choriog... | XM_006712015.3 | 55.4% | 54.7% | (many diffs) |
| 6 | human | 3973 | LHCGR | luteinizing hormone/choriog... | XM_005264309.3 | 54.3% | 54.3% | 0_1ins957 |
| 7 | human | 3973 | LHCGR | luteinizing hormone/choriog... | XM_011532834.2 | 54.3% | 54.3% | 0_1ins957 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2169
- ORF length:
- 2097
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaagcag cggttctcgg cgctgcagct gctgaagctg ctgctgctgc 121 tgcagccgcc gctgccacga gcgctgcgcg aggcgctctg ccctgagccc tgcaactgcg 181 tgcccgacgg cgccctgcgc tgccccggcc ccacggccgg tctcactcga ctatcacttg 241 cctacctccc tgtcaaagtg atcccatctc aagctttcag aggacttaat gaggtcataa 301 aaattgaaat ctctcagatt gattccctgg aaaggataga agctaatgcc tttgacaacc 361 tcctcaattt gtctgaaata ctgatccaga acaccaaaaa tctgagatac attgagcccg 421 gagcatttat aaatcttccc cgattaaaat acttgagcat ctgtaacaca ggcatcagaa 481 agtttccaga tgttacgaag gtcttctcct ctgaatcaaa tttcattctg gaaatttgtg 541 ataacttaca cataaccacc ataccaggaa atgcttttca agggatgaat aatgaatctg 601 taacactcaa actatatgga aatggatttg aagaagtaca aagtcatgca ttcaatggga 661 cgacactgac ttcactggag ctaaaggaaa acgtacatct ggagaagatg cacaatggag 721 ccttccgtgg ggccacaggg ccgaaaacct tggatatttc ttccaccaaa ttgcaggccc 781 tgccgagcta tggcctagag tccattcaga ggctaattgc cacgtcatcc tattctctaa 841 aaaaattgcc atcaagagaa acatttgtca atctcctgga ggccacgttg acttacccca 901 gccactgctg tgcttttaga aacttgccaa caaaagaaca gaatttttca cattccattt 961 ctgaaaactt ttccaaacaa tgtgaaagca cagtaaggaa agtgaataac aaaacacttt 1021 attcttccat gcttgctgag agtgaactga gtggctggga ctatgaatat ggtttctgct 1081 tacccaagac accccgatgt gctcctgaac cagatgcttt taatccctgt gaagatatta 1141 tgggctatga cttccttagg gtcctgattt ggctgattaa tattctagcc atcatgggaa 1201 acatgactgt tctttttgtt ctcctgacaa gtcgttacaa acttacagtg cctcgttttc 1261 tcatgtgcaa tctctccttt gcagactttt gcatggggct ctatctgctg ctcatagcct 1321 cagttgattc ccaaaccaag ggccagtact ataaccatgc catagactgg cagacaggga 1381 gtgggtgcag cactgctggc tttttcactg tattcgcaag tgaactttct gtctacaccc 1441 tcaccgtcat cactctagaa agatggcaca ccatcaccta tgctattcac ctggaccaaa 1501 agctgcgatt aagacatgcc attctgatta tgcttggagg atggctcttt tcttctctaa 1561 ttgctatgtt gccccttgtc ggtgtcagca attacatgaa ggtcagtatt tgcttcccca 1621 tggatgtgga aaccactctc tcacaagtct atatattaac catcctgatt ctcaatgtgg 1681 tggccttctt cataatttgt gcttgctaca ttaaaattta ttttgcagtt cgaaacccag 1741 aattaatggc taccaataaa gatacaaaga ttgctaagaa aatggcaatc ctcatcttca 1801 ccgatttcac ctgcatggca cctatctctt tttttgccat cTCAGCTGCC TTCAAAGTAC 1861 CTCTTATCAC AGTAACCAAC TCTAAAGTTT TACTGGTTCT TTTTTATCCC ATCAATTCTT 1921 GTGCCAATCC ATTTCTGTAT GCAATATTCA CTAAGACATT CCAAAGAGAT TTCTTTCTTT 1981 TGCTGAGCAA ATTTGGCTGC TGTAAACGTC GGGCTGAACT TTATAGAAGG AAAGATTTTT 2041 CAGCTTACAC CTCCAACTGC AAAAATGGCT TCACTGGATC AAATAAGCCT TCTCAATCCA 2101 CCTTGAAGTT GTCCACATTG CACTGTCAAG GTACAGCTCT CCTAGACAAG ACTCGCTACA 2161 CAGAGTGTTA AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2221 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2281 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATAC ACGCAAGGTT GAGCTTGGCA 2341 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t