Construct: ORF TRCN0000488196
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021068.1_s317c1
- DNA Barcode:
- GAAGCCAATACTTTCTTAAATTAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GRM4 (2914)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488196
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2914 | GRM4 | glutamate metabotropic rece... | NM_000841.4 | 99.9% | 100% | 660T>C;1458T>C |
| 2 | human | 2914 | GRM4 | glutamate metabotropic rece... | NM_001256811.2 | 94.7% | 94.7% | 660T>C;1027_1028ins141;1317T>C |
| 3 | human | 2914 | GRM4 | glutamate metabotropic rece... | NM_001256813.3 | 84.3% | 81.1% | (many diffs) |
| 4 | human | 2914 | GRM4 | glutamate metabotropic rece... | NM_001256809.2 | 82.8% | 78.4% | (many diffs) |
| 5 | human | 2914 | GRM4 | glutamate metabotropic rece... | NM_001256812.2 | 81.2% | 81.1% | (many diffs) |
| 6 | human | 2914 | GRM4 | glutamate metabotropic rece... | NM_001282847.2 | 66.1% | 66.2% | 0_1ins924;534T>C |
| 7 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | NM_001291045.1 | 90% | 97.1% | (many diffs) |
| 8 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | XM_006524317.3 | 90% | 97.1% | (many diffs) |
| 9 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | XM_011246475.2 | 90% | 97.1% | (many diffs) |
| 10 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | NM_001013385.2 | 81.1% | 86.7% | (many diffs) |
| 11 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | XM_006524319.3 | 69.7% | 75.1% | (many diffs) |
| 12 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | XM_006524320.3 | 60.1% | 64.6% | (many diffs) |
| 13 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | XM_017317482.1 | 60.1% | 64.6% | (many diffs) |
| 14 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | XM_006524321.3 | 54% | 54.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2808
- ORF length:
- 2736
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcctggg aagagaggct tgggctggtg gtgggcccgg ctgccccttt 121 gcctgctcct cagcctttac ggcccctgga tgccttcctc cctgggaaag cccaaaggcc 181 accctcacat gaattccatc cgcatagatg gggacatcac actgggaggc ctgttcccgg 241 tgcatggccg gggctcagag ggcaagccct gtggagaact taagaaggaa aagggcatcc 301 accggctgga ggccatgctg ttcgccctgg atcgcatcaa caacgacccg gacctgctgc 361 ctaacatcac gctgggcgcc cgcattctgg acacctgctc cagggacacc catgccctcg 421 agcagtcgct gacctttgtg caggcgctca tcgagaagga tggcacagag gtccgctgtg 481 gcagtggcgg cccacccatc atcaccaagc ctgaacgtgt ggtgggtgtc atcggtgctt 541 cagggagctc ggtctccatc atggtggcca acatccttcg cctcttcaag ataccccaga 601 tcagctacgc ctccacagcg ccagacctga gtgacaacag ccgctacgac ttcttctccc 661 gcgtggtgcc ctcggacacg taccaggccc aggccatggt ggacatcgtc cgtgccctca 721 agtggaacta cgtgtccaca gtggcctcgg agggcagcta tggtgagagc ggtgtggagg 781 ccttcatcca gaagtcccgt gaggacgggg gcgtgtgcat cgcccagtcg gtgaagatac 841 cacgggagcc caaggcaggc gagttcgaca agatcatccg ccgcctcctg gagacttcga 901 acgccagggc agtcatcatc tttgccaacg aggatgacat caggcgtgtg ctggaggcag 961 cacgaagggc caaccagaca ggccatttct tctggatggg ctctgacagc tggggctcca 1021 agattgcacc tgtgctgcac ctggaggagg tggctgaggg tgctgtcacg atcctcccca 1081 agaggatgtc cgtacgaggc ttcgaccgct acttctccag ccgcacgctg gacaacaacc 1141 ggcgcaacat ctggtttgcc gagttctggg aggacaactt ccactgcaag ctgagccgcc 1201 acgccctcaa gaagggcagc cacgtcaaga agtgcaccaa ccgtgagcga attgggcagg 1261 attcagctta tgagcaggag gggaaggtgc agtttgtgat cgatgccgtg tacgccatgg 1321 gccacgcgct gcacgccatg caccgtgacc tgtgtcccgg ccgcgtgggg ctctgcccgc 1381 gcatggaccc tgtagatggc acccagctgc ttaagtacat ccgaaacgtc aacttctcag 1441 gcatcgcagg gaaccctgtg accttcaatg agaatggaga tgcgcctggg cgctatgaca 1501 tctaccaata ccagctgcgc aacgattccg ccgagtacaa ggtcattggc tcctggactg 1561 accacctgca ccttagaata gagcggatgc actggccggg gagcgggcag cagctgcccc 1621 gctccatctg cagcctgccc tgccaaccgg gtgagcggaa gaagacagtg aagggcatgc 1681 cttgctgctg gcactgcgag ccttgcacag ggtaccagta ccaggtggac cgctacacct 1741 gtaagacgtg tccctatgac atgcggccca cagagaaccg cacgggctgc cggcccatcc 1801 ccatcatcaa gcttgagtgg ggctcgccct gggccgtgct gcccctcttc ctggccgtgg 1861 tgggcatcgc tgccacgttg ttcgtggtga tcacctttgt gcgctacaac gacacgccca 1921 tcgtcaaggc ctcgggccgt gaactgagct acgtgctgct ggcaggcatc ttcctgtgct 1981 atgccaccac cttcctcatg atcgctgagc ccgaccttgg cacctgctcg ctgcgccgaa 2041 tcttcctggg actagggatg agcatcagct atgcagccct gctcaccaag accaaccgca 2101 tctaccgcat cttcgagcag ggcaagcgct cggtcagtgc cccacgcttc atcagccccg 2161 cctcacagct ggccatcacc ttcagcctca tctcgctgca gctgctgggc atctgtgtgt 2221 ggtttgtggt ggacccctcc cactcggtgg tggacttcca ggaccagcgg acactcgacc 2281 cccgcttcgc caggggtgtg ctcaagtgtg acatctcgga cctgtcgctc atctgcctgc 2341 tgggctacag catgctgctc atggtcacgt gcaccgtgta tgccatcaag acacgcggcg 2401 tgcccgagac cttcaatgag gccaagccca ttggcttcac catgtacacc acttgcatcg 2461 tctggctggc cttcatcccc atcttctttg gcacctcgca gtcggccgac aagctgtaca 2521 tCCAGACGAC GACGCTGACG GTCTCGGTGA GTCTGAGCGC CTCGGTGTCC CTGGGAATGC 2581 TCTACATGCC CAAAGTCTAC ATCATCCTCT TCCACCCGGA GCAGAACGTG CCCAAGCGCA 2641 AGCGCAGCCT CAAAGCCGTC GTTACGGCGG CCACCATGTC CAACAAGTTC ACGCAGAAGG 2701 GCAACTTCCG GCCCAACGGA GAGGCCAAGT CTGAGCTCTG CGAGAACCTT GAGGCCCCAG 2761 CGCTGGCCAC CAAACAGACT TACGTCACTT ACACCAACCA TGCAATCTAG GACCCAGCTT 2821 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2881 CTACGTAGTA ATGAACTAGC CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2941 CTTGTGGAAA GGACGAGAAG CCAATACTTT CTTAAATTAT ACGCGTTAAG TCgacaatca 3001 acctctggat tacaaaattt gtgaaagatt