Construct: ORF TRCN0000488203
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020234.1_s317c1
- DNA Barcode:
- GGCTCTATTTCGACCACATATCCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PHKG2 (5261)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488203
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5261 | PHKG2 | phosphorylase kinase cataly... | NM_000294.3 | 100% | 100% | |
| 2 | human | 5261 | PHKG2 | phosphorylase kinase cataly... | NM_001172432.1 | 90.5% | 88.7% | (many diffs) |
| 3 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | NM_026888.3 | 89.5% | 93.5% | (many diffs) |
| 4 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508167.3 | 89.5% | 93.5% | (many diffs) |
| 5 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_011241891.1 | 89.5% | 93.5% | (many diffs) |
| 6 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508168.2 | 67.5% | 69.4% | (many diffs) |
| 7 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508169.3 | 67.5% | 69.4% | (many diffs) |
| 8 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508170.2 | 66.6% | 69.9% | (many diffs) |
| 9 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508171.2 | 66.6% | 69.9% | (many diffs) |
| 10 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508172.3 | 64.9% | 68.2% | (many diffs) |
| 11 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_017312279.1 | 64.9% | 68.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1290
- ORF length:
- 1218
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgacgctg gacgtggggc cggaggatga gctgcccgac tgggccgccg 121 ccaaagagtt ttaccagaag tacgacccta aggacgtcat cggcagagga gtgagctctg 181 tggtccgccg ttgtgttcat cgagctactg gccacgagtt tgcggtgaag attatggaag 241 tgacagctga gcggctgagt cctgagcagc tggaggaggt gcgggaagcc acacggcgag 301 agacacacat ccttcgccag gtcgccggcc acccccacat catcaccctc atcgattcct 361 acgagtcttc tagcttcatg ttcctggtgt ttgacctgat gcggaaggga gagctgtttg 421 actatctcac agagaaggtg gccctctctg aaaaggaaac caggtccatc atgcggtctc 481 tgctggaagc agtgagcttt ctccatgcca acaacattgt gcatcgagat ctgaagcccg 541 agaatattct cctagatgac aatatgcaga tccgactttc agatttcggg ttctcctgcc 601 acttggaacc tggcgagaag cttcgagagt tgtgtgggac cccagggtat ctagcgccag 661 agatccttaa atgctccatg gatgaaaccc acccaggcta tggcaaggag gtcgacctct 721 gggcctgtgg ggtgatcttg ttcacactcc tggctggctc gccacccttc tggcaccggc 781 ggcagatcct gatgttacgc atgatcatgg agggccagta ccagttcagt tcccccgagt 841 gggatgaccg ttccagcact gtcaaagacc tgatctccag gctgctgcag gtggatcctg 901 aggcacgcct gacagctgag caggccctac agcacccctt ctttGAGCGT TGTGAAGGCA 961 GCCAACCCTG GAACCTCACC CCCCGCCAGC GGTTCCGGGT GGCAGTGTGG ACAGTGCTGG 1021 CTGCTGGACG AGTGGCCCTA AGCACCCATC GTGTACGGCC ACTGACCAAG AATGCACTGT 1081 TGAGGGACCC TTATGCGCTG CGGTCAGTGC GGCACCTCAT CGACAACTGT GCCTTCCGGC 1141 TCTACGGGCA CTGGGTAAAG AAAGGGGAGC AGCAGAACCG GGCGGCTCTC TTTCAGCACC 1201 GGCCCCCTGG GCCTTTTCCC ATCATGGGCC CTGAAGAGGA GGGAGACTCT GCTGCTATAA 1261 CTGAGGATGA GGCCGTGCTT GTGCTGGGCT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG 1321 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1381 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGG 1441 CTCTATTTCG ACCACATATC CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1501 ttgtgaaaga tt