Construct: ORF TRCN0000488233
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020054.1_s317c1
- DNA Barcode:
- CTTTCCTGATTTACCATATGGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADGRG6 (57211)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488233
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | NM_001032395.2 | 99.9% | 99.9% | 3666_3667insG |
| 2 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | XM_006715518.2 | 99.8% | 99.7% | (many diffs) |
| 3 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | NM_198569.3 | 97.6% | 97.6% | (many diffs) |
| 4 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | XM_006715516.2 | 97.6% | 97.5% | (many diffs) |
| 5 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | XM_006715517.2 | 97.6% | 97.5% | (many diffs) |
| 6 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | XM_011535964.1 | 97.5% | 97.4% | (many diffs) |
| 7 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | NM_001032394.2 | 95.1% | 95.4% | 3488_3533del;3579_3580ins134 |
| 8 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | NM_020455.5 | 93% | 93.3% | 1138_1221del;3572_3617del;3663_3664ins134 |
| 9 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | XM_005267061.3 | 92.9% | 93.1% | (many diffs) |
| 10 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | XM_017011085.1 | 86.6% | 86.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3741
- ORF length:
- 3669
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgatgttt cgctcagatc gaatgtggag ctgccattgg aaatggaagc 121 ccagtcctct cctgttctta tttgctttat atatcatgtg tgttcctcac tcagtgtggg 181 gatgtgccaa ctgccgagtg gttttgtcca acccttctgg gacctttact tctccatgct 241 accctaacga ctacccaaac agccaggctt gcatgtggac gctccgagcc cccaccggtt 301 atatcattca gataacattt aacgactttg acattgaaga agctcccaat tgcatttatg 361 actcattatc ccttgataat ggagagagcc agactaaatt ttgtggagca actgccaaag 421 gcctatcatt taactcaagt gcgaatgaga tgcatgtgtc cttttcaagt gactttagca 481 tccagaagaa aggtttcaat gccagctaca tcagagttgc cgtgtcctta aggaatcaaa 541 aggtcatttt accccagaca tcagatgctt accaggtatc tgttgcaaaa agcatctcta 601 ttccagagct cagtgctttc acactctgct ttgaagcaac caaagttggc catgaagaca 661 gtgattggac agctttctcc tactcaaatg catccttcac acaattgctc agttttggaa 721 aggccaagag tggctacttt ctatccattt ctgattcaaa atgtttgttg aataatgcat 781 tacctgtcaa agaaaaagaa gacatttttg cagaaagctt tgaacagctc tgccttgttt 841 ggaataattc tttgggctct attggtgtaa atttcaaaag aaactatgaa acagttccat 901 gtgattctac cattagtaaa gttattcctg ggaatgggaa attgttgttg ggctccaatc 961 aaaatgaaat tgtctctcta aaaggggaca tttataactt tcgactttgg aattttacca 1021 tgaatgccaa aatcctctcc aacctcagct gtaatgtgaa agggaatgta gtcgactggc 1081 aaaatgactt ctggaatatc ccaaacctag ctctgaaagc tgaaagcaac ctaagctgtg 1141 gttcctacct gatcccgctc ccagcagcag aactggccag ctgtgcagac ctggggaccc 1201 tctgtcaaga tggaattatc tatagaatat ccgtagtgat tcagaacatc cttcgtcacc 1261 ctgaggtaaa agtacagagc aaggtggcag aatggctcaa ttcaaccttc caaaattgga 1321 actacacggt ttatgtcgtt aatatcagtt ttcacctgag tgctggagag gacaagatta 1381 aagtcaagag aagccttgag gatgagccaa ggttggtgct ttgggccctt ctagtttaca 1441 atgctaccaa caatactaat ttagaaggaa aaatcattca gcagaagctc ctaaaaaata 1501 atgagtcctt ggatgaaggc ttgaggctac atacagtgaa tgtgagacaa ctgggtcatt 1561 gtcttgccat ggaggaaccc aaaggctact actggccatc tatccaacct tctgaatacg 1621 ttcttccttg tccagacaag cctggctttt ctgcttctcg gatatgtttt tacaatgcta 1681 ccaacccatt ggtaacctac tggggacctg ttgatatctc caactgttta aaagaagcaa 1741 atgaagttgc taaccagatt ttaaatttaa ctgctgatgg gcagaactta acctcagcca 1801 atattaccaa cattgtggaa caggtcaaaa gaattgtgaa taaagaagaa aacattgata 1861 taacacttgg ctcaactcta atgaatatat tttctaatat cttaagcagt tcagacagtg 1921 acttgcttga gtcatcttct gaagctttaa aaacaattga tgaattggcc ttcaagatag 1981 acctaaatag cacatcacat gtgaatatta caactcggaa cttggctctc agcgtatcat 2041 ccctgttacc agggacaaat gcaatttcaa attttagcat tggtcttcca agcaataatg 2101 aatcgtattt ccagatggat tttgagagtg gacaagtgga tccactggca tctgtaattt 2161 tgcctccaaa cttacttgag aatttaagtc cagaagattc tgtattagtt agaagagcac 2221 agtttacttt cttcaacaaa actggacttt tccaggatgt aggaccccaa agaaaaactt 2281 tagtgagtta tgtgatggcg tgcagtattg gaaacattac tatccagaat ctgaaggatc 2341 ctgttcaaat aaaaatcaaa catacaagaa ctcaggaagt gcatcatccc atctgtgcct 2401 tctgggatct gaacaaaaac aaaagttttg gaggatggaa cacgtcagga tgtgttgcac 2461 acagagattc agatgcaagt gagacagtct gcctgtgtaa ccacttcaca cactttggag 2521 ttctgatgga ccttccaaga agtgcctcac agttagatgc aagaaacact aaagtcctca 2581 ctttcatcag ctatattggg tgtggaatat ctgctatttt ttcagcagca actctcctga 2641 catatgttgc ttttgagaaa ttgcgaaggg attatccctc caaaatcttg atgaacctga 2701 gcacagccct gctgttcctg aatctcctct tcctcctaga tggctggatc acctccttca 2761 atgtggatgg actttgcatt gctgttgcag tcctgttgca tttcttcctt ctggcaacct 2821 ttacctggat ggggctagaa gcaattcaca tgtacattgc tctagttaaa gtatttaaca 2881 cttacattcg ccgatacatt ctaaaattct gcatcattgg ctggggtttg cctgccttag 2941 tggtgtcagt tgttctagcg agcagaaaca acaatgaagt ctatggaaaa gaaagttatg 3001 ggaaagaaaa aggtgatgaa ttctgttgga ttcaagatcc agtcatattt tatgtgacct 3061 gtgctgggta ttttggagtc atgttttttc tgaacattgc catgttcatt gtggtaatgg 3121 tgcagatctg tgggaggaat ggcaagagaa gcaaccggac cctgagagaa gaagtgttaa 3181 ggaacctgcg cagtgtggtt agcttgacct ttctgttggg catgacatgg ggttttgcat 3241 tctttgcctg gggaccctta aatatcccct tcatgtacct cttctccatc ttcaattcat 3301 tacaaggctt atttatattc atcttccact gtgctatgaa ggagaatgtt cagaaacagt 3361 ggcggcggca tctctgctgt ggtagatttc ggttagcaga taactcagat tGGAGTAAGA 3421 CAGCTACCAA TATCATCAAG AAAAGTTCTG ATAATCTAGG AAAATCTTTG TCTTCAAGCT 3481 CCATTGGTTC CAACTCAACC TATCTTACAT CCAAATCTAA ATCCAGCTCT ACCACCTATT 3541 TCAAAAGGAA TAGCCACACA GACAGTGCTT CCATGGACAA GTCCTTGTCA AAACTGGCCC 3601 ATGCTGATGG AGATCAAACA TCAATCATCC CTGTCCATCA GGTCATTGAT AAGGTCAAGG 3661 GTTATTGCAA TGCTCATTCA GACAACTTCT ATAAAAATAT TATCATGTCA GACACCTTCA 3721 GCCACAGCAC AAAGTTTGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 3781 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 3841 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTTTCCT GATTTACCAT 3901 ATGGCAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt